About: Analysis of virus-specific RNA species and proteins in Freon-113 preparations of the Borna disease virus

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About: Analysis of virus-specific RNA species and proteins in Freon-113 preparations of the Borna disease virus Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Analysis of virus-specific RNA species and proteins in Freon-113 preparations of the Borna disease virus
Creator	Herzog, S Richt, J Becht, H Clements, Pyper, Wahn, K
source	PMC
abstract	Treatment of homogenates from Borna disease virus (BDV)-infected brain tissue or cell cultures with Freon-113 yielded infectious particles with a buoyant density of 1.16–1.22 g/ml. Positive- and negative-stranded BDV-specific RNA species as well as three virus-specific proteins, known to be present in BDV-infected cell extracts, were demonstrated in these Freon-treated fractions. When the Freon-purified virus preparations were treated with RNase A prior to RNA extraction, only negative-stranded, genomic RNA was detected in Northern blot hybridizations using sense and antisense RNA probes. These data substantiate that BDV is a negative-stranded RNA virus.
has issue date	1993-01-01(xsd:dateTime)
bibo:doi	10.1007/bf00579625
bibo:pmid	8283962
has license	no-cc
sha1sum (hex)	55299b59e84aecb71f929e0489c31f762d593e04
schema:url	https://doi.org/10.1007/bf00579625
resource representing a document's title	Analysis of virus-specific RNA species and proteins in Freon-113 preparations of the Borna disease virus
has PubMed Central identifier	PMC7086879
has PubMed identifier	8283962
schema:publication	Med Microbiol Immunol
resource representing a document's body	covid:55299b59e84aecb71f929e0489c31f762d593e04#body_text
is schema:about of	named entity 'RNA' named entity 'Borna' named entity 'species' named entity 'RNASE A' named entity 'buoyant density' named entity 'RNA' named entity 'brain tissue' named entity 'RNA' named entity 'RNA virus' named entity 'BDV' named entity 'BDV' named entity 'RNA extraction' named entity 'cell cultures' named entity 'Freon' named entity 'proteins' named entity 'species' named entity 'RNA' named entity 'BDV' named entity 'positive-stranded' named entity 'tRNA' named entity '1/3' named entity 'positive-stranded' named entity 'antibodies' named entity 'RNAs' named entity 'RNase' named entity 'Freon' named entity 'Infection' named entity 'sperm' named entity 'RNase' named entity 'digestion' named entity 'RNA' named entity 'infectivity' named entity 'RNA' named entity 'virus' named entity '10.0' named entity 'Freon' named entity 'riboprobe' named entity 'Germany' named entity 'solvent' named entity 'Amersham' named entity 'infection' named entity 'electrophoresis' named entity 'lysed' named entity 'BDV' named entity 'formaldehyde' named entity 'infectivity' named entity 'Promega' named entity 'BDV' named entity 'Freon' named entity 'virus-like particles' named entity 'Viral' named entity 'glycine' named entity 'staining' named entity 'electrophoresed' named entity 'RNase A' named entity 'Freon' named entity 'vector' named entity 'BDV' named entity 'RNA' named entity 'RNA' named entity 'mRNA' named entity 'Hannover' named entity 'protein' named entity 'RNase' named entity 'Madin-Darby canine kidney' named entity '8.5' named entity 'DMEM' named entity 'homogenizer' named entity 'RNA probes' named entity 'RNA' named entity 'Germany' named entity 'antibodies' named entity 'specific antigens' named entity 'positive-stranded' named entity 'BDV' named entity 'F r'

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