About: Simultaneous and visual detection of infectious bronchitis virus and Newcastle disease virus by multiple LAMP and lateral flow dipstick

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About: Simultaneous and visual detection of infectious bronchitis virus and Newcastle disease virus by multiple LAMP and lateral flow dipstick Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Simultaneous and visual detection of infectious bronchitis virus and Newcastle disease virus by multiple LAMP and lateral flow dipstick
Creator	Wang, Hongning Xiang, Rong Zhou, Long Kang, Zhuangzhuang Mei, Xueran Wu, Xuan Zhai, Xiwen Zuo, Lei Song, Zengxu
source	Elsevier; Medline; PMC
abstract	ABSTRACT Infectious bronchitis virus (IBV) and Newcastle disease virus (NDV) are both important viruses seriously affecting poultry industry worldwide. In this study, reverse-transcription LAMP (RT-LAMP) was combined with lateral flow dipstick (LFD) forming a novel detection tool which could simultaneously detect IBV and NDV visually. Primers targeted the 5′-untranslated region (5′-UTR) of IBV genome and the conserved region of NDV large polymerase gene (LP). The specificity and sensitivity of this multiple reverse transcription-LAMP-LFD (mRT-LAMP-LFD) assay were compared with those of conventional RT-PCR, nested RT-PCR (nRT-PCR), quantification RT-PCR (qRT-PCR), and RT-LAMP monitored by electrophoresis. No non-specific amplifications were observed when the assays were tested with unrelated viruses. According to the sensitivity study, when detecting IBV or NDV alone, the lowest detection limits of mRT-LAMP-LFD were 100.8 IBV RNA copies/reaction and 100.7 NDV RNA copies/reaction. Furthermore, when detecting IBV and NDV simultaneously, the lowest detection limit was the same as that of the single detection assays. In the clinical sample study, mRT-LAMP-LFD performed the best among these assays. When tested with IBV or NDV single infected samples, the mean detection rates were 98.65% and 97.25%, respectively. In the IBV and NDV co-infected sample study, the mean detection rates of IBV and NDV were both 95%. This study showed that mRT-LAMP-LFD was a promising qualitative detection tool suitable for field single or multiple IBV and NDV detection.
has issue date	2019-11-01(xsd:dateTime)
bibo:doi	10.3382/ps/pez372
bibo:pmid	31265112
has license	els-covid
sha1sum (hex)	55a9ed6ed8fbd77c18d5d42e3dba9121a22e8e07
schema:url	https://doi.org/10.3382/ps/pez372
resource representing a document's title	Simultaneous and visual detection of infectious bronchitis virus and Newcastle disease virus by multiple LAMP and lateral flow dipstick
has PubMed Central identifier	PMC7107193
has PubMed identifier	31265112
schema:publication	Poultry Science
resource representing a document's body	covid:55a9ed6ed8fbd77c18d5d42e3dba9121a22e8e07#body_text
is schema:about of	named entity 'VISUAL' named entity 'CLINICAL' named entity 'THESE' named entity 'NOVEL' named entity 'WORLDWIDE' named entity '0.8' named entity 'GENOME' named entity 'PERFORMED' named entity 'LOWEST' named entity 'SENSITIVITY' named entity 'WAS A' named entity 'FORMING' named entity 'LIMITS' named entity 'DETECTING' named entity 'CONVENTIONAL' named entity 'DETECT' named entity 'COMPARED' named entity 'TESTED' named entity 'sensitivity' named entity 'NDV' named entity 'single' named entity 'NDV' named entity 'genome' named entity 'targeted' named entity 'lateral' named entity 'Simultaneous' named entity 'INFECTIOUS BRONCHITIS VIRUS' named entity 'SIMULTANEOUS' named entity 'RT-PCR' named entity 'QUANTIFICATION' named entity 'POULTRY INDUSTRY' named entity 'TOOL' named entity 'UNRELATED' named entity 'BEST' named entity 'REVERSE TRANSCRIPTION' named entity 'NEWCASTLE DISEASE VIRUS' named entity 'DETECTION' named entity 'LATERAL' named entity 'LAMP' named entity 'MULTIPLE' named entity '0.7' named entity 'VIRUSES' named entity 'OBSERVED' named entity 'DIPSTICK' named entity 'FLOW' named entity 'MEAN' named entity 'NON-SPECIFIC' named entity 'TARGETED' named entity 'NESTED' named entity 'ELECTROPHORESIS' named entity 'SUITABLE' named entity 'FLOW' named entity 'DIPSTICK' named entity 'NEWCASTLE DISEASE VIRUS' named entity 'ASSAY' named entity 'DETECTION' named entity 'LARGE' named entity 'COMBINED' named entity 'IMPORTANT' named entity 'MONITORED' named entity 'SAMPLES' named entity 'POLYMERASE GENE' named entity 'REVERSE-TRANSCRIPTION' named entity 'LAMP' named entity 'IBV' named entity 'RATES' named entity 'RNA' named entity 'ACCORDING' named entity 'QUALITATIVE' named entity 'SAME AS' named entity 'UNTRANSLATED REGION' named entity 'STUDY' named entity 'SPECIFICITY'

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