About: Impedimetric cell-based biosensor for real-time monitoring of cytopathic effects induced by dengue viruses

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About: Impedimetric cell-based biosensor for real-time monitoring of cytopathic effects induced by dengue viruses Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Impedimetric cell-based biosensor for real-time monitoring of cytopathic effects induced by dengue viruses
Creator	Toh, Chee-Seng Chan, Kwai Chow, Vincent Cheng, Ming Lau, Suk
Source	Elsevier; Medline; PMC
abstract	Abstract We describe an impedimetric cell-based biosensor constructed from poly-l-lysine (PLL)-modified screen-printed carbon electrode for real-time monitoring of dengue virus (DENV) infection of surface-immobilized baby hamster kidney (BHK-21) fibroblast cells. Cytopathic effects (CPE) induced by DENV-2 New Guinea C strain (including degenerative morphological changes, detachment, membrane degradation and death of host cells), were reflected by drastic decrease in impedance signal response detected as early as ~30 hours post-infection (hpi). In contrast, distinct CPE by conventional microscopy was evident only at ~72hpi at the corresponding multiplicity of infection (MOI) of 10. A parameter that describes the kinetics of cytopathogenesis, CIT50, which refers to the time taken for 50% reduction in impedance signal response, revealed an inverse linear relationship with virus titer and MOI. CIT50 values were also delayed by 31.5h for each order of magnitude decrease in MOI. Therefore, based on the analysis of CIT50, the virus titer of a given sample can be determined from the measured impedance signal response. Furthermore, consistent impedance results were also obtained with clinical isolates of the four DENV serotypes verified by RT-PCR and cycle sequencing. This impedimetric cell-based biosensor represents a label-free and continuous approach for the dynamic measurement of cellular responses toward DENV infection, and for detecting the presence of infectious viral particles.
has issue date	2015-08-15(xsd:dateTime)
bibo:doi	10.1016/j.bios.2015.03.018
bibo:pmid	25794961
has license	els-covid
sha1sum (hex)	58eca001fcf7699a968055cf8adbcac6aba11f63
schema:url	https://doi.org/10.1016/j.bios.2015.03.018
resource representing a document's title	Impedimetric cell-based biosensor for real-time monitoring of cytopathic effects induced by dengue viruses
has PubMed Central identifier	PMC7126431
has PubMed identifier	25794961
schema:publication	Biosensors and Bioelectronics
resource representing a document's body	covid:58eca001fcf7699a968055cf8adbcac6aba11f63#body_text
is schema:about of	named entity 'refers' named entity 'CPE' named entity 'detected' named entity 'sequencing' named entity 'dynamic' covid:arg/58eca001fcf7699a968055cf8adbcac6aba11f63 named entity 'infection' named entity 'detecting' named entity 'linear relationship' named entity 'virus' named entity 'modified' named entity 'electrode' named entity 'DENV' named entity 'contrast' named entity 'biosensor' named entity 'dengue virus' named entity 'BHK-21' named entity 'virus' named entity 'DENV' named entity 'hpi' named entity 'MOI' named entity 'host cells' named entity 'infection' named entity 'viral particles' named entity 'titer' named entity 'poly-L-lysine' named entity 'DENV' named entity 'MOI' named entity 'w/v' named entity 'infection' named entity 'endothelial cells' named entity 'PBS' named entity 'inoculation' named entity 'overpotential' named entity 'DENV' named entity 'primers' named entity '37°C' named entity 'incubation' named entity 'annealing' named entity 'electrode' named entity 'carbon' named entity 'morphological' named entity 'TC10' named entity 'infection' named entity 'w/v' named entity 'sulfate' named entity 'cationic polymer' named entity 'PLL' named entity 'MOI' named entity 'sterilization' named entity 'endocytic vesicle' named entity 'plaque-forming unit' named entity 'RNA' named entity 'endothelial cells' named entity 'PLL' named entity 'pipette' named entity 'ebola virus' named entity 'Vero' named entity 'DENV' named entity 'carbon' named entity 'RT-PCR' named entity 'DENV' named entity 'RPMI' named entity 'cDNA' named entity 'complement' named entity 'biosensor' named entity 'DENV' named entity 'potentiostat' named entity 'tight junctions' named entity 'RT-PCR' named entity 'morphological' named entity 'biosensor' named entity 'DENV' named entity 'electrode' named entity 'hpi' named entity 'BHK-21 cells' named entity 'PLL'

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