About: Evaluation of an accelerated hydrogen peroxide disinfectant to inactivate porcine epidemic diarrhea virus in swine feces on aluminum surfaces under freezing conditions

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About: Evaluation of an accelerated hydrogen peroxide disinfectant to inactivate porcine epidemic diarrhea virus in swine feces on aluminum surfaces under freezing conditions Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Evaluation of an accelerated hydrogen peroxide disinfectant to inactivate porcine epidemic diarrhea virus in swine feces on aluminum surfaces under freezing conditions
Creator	Wang, Chong Zhang, Jianqiang Ramirez, Alejandro Baker, Kimberlee Holtkamp, Derald Thomas, Paul Karriker, Locke
Source	Medline; PMC
abstract	BACKGROUND: Since its emergence in 2013, porcine epidemic diarrhea virus (PEDV) spread rapidly throughout the country due, in part, to contaminated livestock trailers. The objective of this study was to test the efficacy of an accelerated hydrogen peroxide (AHP) disinfectant for inactivating PEDV in swine feces on metal surfaces under freezing conditions. One 15.24 X 15.24 X 2.54 cm aluminum coupon, contaminated with swine feces, and randomly matched to one pig was the experimental unit. Eight treatment groups representing two AHP concentrations (1:16 and 1:32) in a 10% propylene glycol solution, two contact times in a -10 °C freezer (40 min and 60 min), and two levels of fecal contamination (5 mL and 10 mL) in addition to negative and positive control groups were evaluated. Forty 3-week-old pigs, intragastrically inoculated with the contents of the coupons after treatment, were used as a bioassay to determine the infectivity of PEDV after treatment. Infectivity was determined by detection of virus with a nucleocapsid (N) gene-based quantitative real-time reverse transcription polymerase chain reaction (RT-qPCR) on rectal swabs collected from the inoculated pigs on days three and seven post-inoculation. RESULTS: All post-treatment swabs from the negative control coupons were negative for PEDV via RT-qPCR. All post-treatment swabs collected from coupons in the AHP disinfectant treatment groups and the positive control group were positive for PEDV via RT-qPCR. For the bioassay, no rectal swabs from pigs in the negative control (0 of 4) or the AHP disinfectant treatment groups (0 of 32) were positive for PEDV. Rectal swabs from all pigs within the positive control group (4 of 4) were positive for PEDV by RT-qPCR. CONCLUSIONS: Under the conditions of this study, 1:16 and 1:32 dilutions of the AHP disinfectant successfully inactivated PEDV in swine feces on metal surfaces when applied at -10 °C with 40 or 60 min of contact time. This study also suggests that a positive RT-qPCR result for PEDV on an environmental sample should be expected when the AHP disinfectant is applied under freezing conditions, but does not necessarily indicate that an infectious dose of PEDV remains after disinfection. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi: 10.1186/s12917-017-1300-4) contains supplementary material, which is available to authorized users.
has issue date	2017-12-01(xsd:dateTime)
bibo:doi	10.1186/s12917-017-1300-4
bibo:pmid	29191202
has license	cc-by
sha1sum (hex)	5b1c9c575d37e7026b0c2c0fdfe309b7a06a528a
schema:url	https://doi.org/10.1186/s12917-017-1300-4
resource representing a document's title	Evaluation of an accelerated hydrogen peroxide disinfectant to inactivate porcine epidemic diarrhea virus in swine feces on aluminum surfaces under freezing conditions
has PubMed Central identifier	PMC5709983
has PubMed identifier	29191202
schema:publication	BMC Vet Res
resource representing a document's body	covid:5b1c9c575d37e7026b0c2c0fdfe309b7a06a528a#body_text
is schema:about of	named entity 'DISINFECTANT' named entity 'CONDITIONS' named entity 'treatment groups' named entity 'feces' named entity 'positive control' named entity 'determine' named entity 'efficacy' named entity 'conditions' named entity 'SURFACES' named entity 'TO TEST' named entity 'SOLUTION' named entity 'QPCR' named entity 'FECES' named entity 'CONTROL GROUPS' named entity 'FREEZER' named entity 'USED' named entity '10%' named entity 'EMERGENCE' named entity 'GROUPS' named entity 'LEVELS' named entity 'PORCINE EPIDEMIC DIARRHEA VIRUS' named entity 'OLD' named entity 'TRAILERS' named entity 'PROPYLENE GLYCOL' named entity 'TREATMENT' named entity 'SPREAD' named entity 'DISINFECTANT' named entity 'OBJECTIVE' named entity 'BACKGROUND' named entity 'SWABS' named entity 'COUNTRY' named entity 'INFECTIVITY' named entity 'DETERMINE' named entity 'CONTENTS' named entity 'WEEK' named entity 'CONCENTRATIONS' named entity 'NEGATIVE' named entity 'ALUMINUM' named entity 'EVALUATED' named entity 'DETECTION OF VIRUS' named entity 'PIGS' named entity 'FREEZING' named entity 'PORCINE EPIDEMIC DIARRHEA VIRUS' named entity 'EVALUATION' named entity 'MATCHED' named entity 'NUCLEOCAPSID' named entity 'EXPERIMENTAL UNIT' named entity 'DAYS' named entity 'REAL-TIME REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION' named entity 'BIOASSAY' named entity 'DETERMINED BY' named entity 'FECAL' named entity 'POSITIVE CONTROL' named entity 'LIVESTOCK' named entity 'ADDITION' named entity 'EFFICACY' named entity 'FREEZING' named entity 'ACCELERATED' named entity 'PIG' named entity 'ALUMINUM' named entity 'SWINE' named entity 'STUDY' named entity 'POST' named entity 'CONTAMINATION' named entity 'QUANTITATIVE' named entity 'METAL' named entity 'MIN' named entity 'CONTACT' named entity 'CONDITIONS' named entity 'AFTER TREATMENT' named entity 'HYDROGEN PEROXIDE' named entity 'GENE' named entity 'HYDROGEN PEROXIDE' named entity 'FECES' named entity 'ITS'

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