About: A surface plasmon resonance-based assay for small molecule inhibitors of human cyclophilin A

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About: A surface plasmon resonance-based assay for small molecule inhibitors of human cyclophilin A Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	A surface plasmon resonance-based assay for small molecule inhibitors of human cyclophilin A
Creator	Dunsmore, Colin Malone, Kirk Patterson, Alan Turner, Nicholas Walkinshaw, Malcolm Wear, Martin
Source	Elsevier; Medline; PMC
abstract	Abstract A simple protocol for generating a highly stable and active surface plasmon resonance (SPR) sensor surface of recombinant human hexahistidine cyclophilin A (His-CypA) is described. The sensor surface was sensitive and stable enough to allow, for the first time, the screening and ranking of several novel small-molecule (M r ∼250–500Da) ligands in a competition binding assay with cyclosporin A (CsA). It also allowed us to accurately determine the kinetic rate constants for the interaction between His-CypA and CsA. His-CypA was first captured on a Ni2+–nitrilotriacetic acid (NTA) sensor chip and was then briefly covalently stabilized, coupling via primary amines. The significant baseline drift observed due to dissociation of weakly bound His-CypA from the Ni2+–NTA moiety was eliminated, resulting in a surface that was stable for at least 36h. In addition, immobilized protein activity levels were high, typically between 85 and 95%, assayed by the interaction between His-CypA and CsA. The mean equilibrium dissociation constant for CsA (K dCsA) binding to the immobilized His-CypA was 23±6nM, with on and off rates of 0.53±0.1μM−1 s−1 and 1.2±0.1 (×10−2) s−1, respectively. These values agree well with the values for the corresponding binding constants determined from steady-state and kinetic fluorescence titrations in solution.
has issue date	2005-10-15(xsd:dateTime)
bibo:doi	10.1016/j.ab.2005.06.037
bibo:pmid	16102717
has license	els-covid
sha1sum (hex)	5daa5ef3f12ae1df45f5c1d91f2d98465a30a72b
schema:url	https://doi.org/10.1016/j.ab.2005.06.037
resource representing a document's title	A surface plasmon resonance-based assay for small molecule inhibitors of human cyclophilin A
has PubMed Central identifier	PMC7094681
has PubMed identifier	16102717
schema:publication	Analytical Biochemistry
resource representing a document's body	covid:5daa5ef3f12ae1df45f5c1d91f2d98465a30a72b#body_text
is schema:about of	named entity 'CYCLOPHILIN A' named entity 'BASED' named entity 'HUMAN' named entity '0.1' named entity 'SENSOR' named entity 'BSA' named entity 'RANKING' named entity 'VALUES' named entity 'ACTIVITY LEVELS' named entity 'SIMPLE' named entity 'SOLUTION' named entity 'DISSOCIATION CONSTANT' named entity 'RATE' named entity 'CYPA' named entity 'DCSA' named entity 'SURFACE' named entity '1.2' named entity 'BINDING ASSAY' named entity 'SULFOXIDE' named entity 'WAS SENSITIVE' named entity 'DISSOCIATION' named entity 'NITRILOTRIACETIC ACID' named entity 'HUMAN' named entity 'STABLE' named entity 'DESCRIBED' named entity 'ACTIVE' named entity '500' named entity 'BOVINE SERUM ALBUMIN' named entity 'SURFACE PLASMON RESONANCE' named entity 'TITRATIONS' named entity 'HIGH' named entity 'RESULTING IN' named entity 'SURFACE PLASMON RESONANCE' named entity 'WWW' named entity 'SCREENING' named entity 'SENSOR CHIP' named entity 'EQUILIBRIUM' named entity 'FLUORESCENCE' named entity 'ELIMINATED' named entity 'MOLECULE' named entity 'ITC' named entity 'DRIFT' named entity 'CYCLOPHILIN A' named entity 'STABILIZED' named entity 'AGREE' named entity '214' named entity 'SMALL' named entity 'CYCLOSPORIN A' named entity 'ALLOW' named entity 'THESE' named entity 'NTA' named entity 'OBSERVED' named entity 'DETERMINE' named entity 'DUE TO' named entity 'SMALL MOLECULE' named entity 'INHIBITORS' named entity 'MOIETY' named entity 'COMPETITION BINDING' named entity 'IMMOBILIZED PROTEIN' named entity 'HIGHLY' named entity 'PROTEIN ACTIVITY' named entity 'BOUND' named entity 'ASSAY' named entity 'LIGANDS' named entity 'GENERATING' named entity 'HEXAHISTIDINE' named entity 'ISOTHERMAL TITRATION CALORIMETRY' named entity 'NOVEL' named entity 'BASELINE' named entity 'KINETIC' named entity 'HIS-' named entity 'ANALYTICAL BIOCHEMISTRY' named entity 'COM' named entity '226' named entity 'PROTOCOL' named entity 'COUPLING'

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