About: Increased talin–vinculin spatial proximities in livers in response to spotted fever group rickettsial and Ebola virus infections

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Increased talin–vinculin spatial proximities in livers in response to spotted fever group rickettsial and Ebola virus infections
Creator	Chang, Qing Ksiazek, Thomas Drelich, Aleksandra Judy, Barbara Li, • Rossi, Shannan Yang, • Liu, Yakun Saito, Tais Shelite, Thomas Su, Zhengchen Tang, Shao-Jun Xiao, Jie Zhang, Ben Zheng, Junying Zhou, Yixuan Bei, • Bhupendra Kaphalia, • Gong, • Maki, •
source	Medline; PMC
abstract	Talin and vinculin, both actin-cytoskeleton-related proteins, have been documented to participate in establishing bacterial infections, respectively, as the adapter protein to mediate cytoskeleton-driven dynamics of the plasma membrane. However, little is known regarding the potential role of the talin–vinculin complex during spotted fever group rickettsial and Ebola virus infections, two dreadful infectious diseases in humans. Many functional properties of proteins are determined by their participation in protein–protein complexes, in a temporal and/or spatial manner. To resolve the limitation of application in using mouse primary antibodies on archival, multiple formalin-fixed mouse tissue samples, which were collected from experiments requiring high biocontainment, we developed a practical strategic proximity ligation assay (PLA) capable of employing one primary antibody raised in mouse to probe talin–vinculin spatial proximal complex in mouse tissue. We observed an increase of talin–vinculin spatial proximities in the livers of spotted fever Rickettsia australis or Ebola virus-infected mice when compared with mock mice. Furthermore, using EPAC1-knockout mice, we found that deletion of EPAC1 could suppress the formation of spatial proximal complex of talin–vinculin in rickettsial infections. In addition, we observed increased colocalization between spatial proximity of talin–vinculin and filamentous actin-specific phalloidin staining in single survival mouse from an ordinarily lethal dose of rickettsial or Ebola virus infection. These findings may help to delineate a fresh insight into the mechanisms underlying liver specific pathogenesis during infection with spotted fever rickettsia or Ebola virus in the mouse model.
has issue date	2020-04-01(xsd:dateTime)
bibo:doi	10.1038/s41374-020-0420-9
bibo:pmid	32238906
has license	no-cc
sha1sum (hex)	5ffde86345953f0db21fa8aa504287783e020634
schema:url	https://doi.org/10.1038/s41374-020-0420-9
resource representing a document's title	Increased talin–vinculin spatial proximities in livers in response to spotted fever group rickettsial and Ebola virus infections
has PubMed Central identifier	PMC7111589
has PubMed identifier	32238906
schema:publication	Lab Invest
resource representing a document's body	covid:5ffde86345953f0db21fa8aa504287783e020634#body_text
is schema:about of	named entity 'Ebola' named entity 'infection' named entity 'Many' named entity 'EPAC1' named entity 'mouse' named entity 'collected' named entity 'specific' named entity 'group' named entity 'mouse' named entity 'TISSUE' named entity 'PROXIMAL' named entity 'UNDERLYING' named entity 'STAINING' named entity 'TALIN' named entity 'FUNCTIONAL' named entity 'MULTIPLE' named entity 'PROBE' named entity 'PROTEINS' named entity 'SPATIAL' named entity 'THEIR' named entity 'FINDINGS' named entity 'EBOLA VIRUS' named entity 'PROTEIN ' named entity 'spotted fever' named entity 'mediate' named entity 'resolve' named entity 'complex' named entity 'lethal dose' named entity 'manner' named entity 'deletion' named entity 'cytoskeleton' named entity 'vinculin' named entity 'talin' named entity 'cytoskeleton' named entity 'talin' named entity 'primary antibody' named entity 'Rickettsia australis' named entity 'rickettsial infections' named entity 'talin' named entity 'talin' named entity 'rickettsia' named entity 'plasma membrane' named entity 'vinculin' named entity 'talin' named entity 'mice' named entity 'formalin-fixed' named entity 'adapter protein' named entity 'spotted fever' named entity 'spotted fever group' named entity 'rickettsial' named entity 'spatial' named entity 'BSL4' named entity 'murine' named entity 'DAPI' named entity 'EPAC1' named entity 'protein' named entity 'vinculin' named entity 'Vinculin' named entity 'BSL3' named entity 'carcinogenesis' named entity '0.01' named entity 'infectious diseases'

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