About: Development and clinical application of a rapid and sensitive loop-mediated isothermalamplification test for SARS-CoV-2 infection

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Development and clinical application of a rapid and sensitive loop-mediated isothermalamplification test for SARS-CoV-2 infection Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Development and clinical application of a rapid and sensitive loop-mediated isothermalamplification test for SARS-CoV-2 infection
Creator	Zhang, Lei Li, Shan Wang, Xiaoming Hou, Tieying Hu, Xuejiao Chen, Jierong Deng, Qianyun Fang, Zhixin Hu, A Li, Haijian Li, Junmin Li, Wenmin Wang, Zixia Yu, Pan Zhang, Xiqin Zhao, Yunhu
source	MedRxiv
abstract	Background The outbreak of SARS-CoV-2 urgently requires sensitive and convenient COVID-19 diagnostics to assure the containment and timely treatment of patients. We aimed to develop and validate a novel reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay to detect SARS-CoV-2 in both qualified laboratories and point-of-care settings. Methods Patients with suspected COVID-19 and close contacts between Jan 26 and April 8, 2020, were recruited from two hospitals. Respiratory samples were collected and tested with LAMP and the results were compared with those obtained by RT-qPCR. The inconsistent samples between these two methods were subjected to next-generation sequencing for confirmation. In addition, we tested the RT-LAMP on an asymptomatic COVID-19 carrier and patients with other respiratory viral infections. Results We finally collected a cohort of 129 cases (329 nasopharyngeal swabs) and the independent cohort of 76 patients (152 nasopharyngeal swabs and sputum samples). RT-LAMP was validated to be accurate (overall sensitivity and specificity: 88.89% and 99.00%; positive and negative predictive values: 94.74% and 97.78%) and diagnostically useful (positive and negative likelihood ratios: 88.89 and 0.11). RT-LAMP showed an increased sensitivity (88.89% vs 81.48%) and high consistency (kappa 0.92) compared with RT-qPCR for SARS-CoV-2 screening while requiring only constant temperature heating and visual inspection. The median time required for RT-LAMP was less than 1 h from sample to result. Further analyses indicated that RT-LAMP was feasible for asymptomatic patients and did not cross-react with other respiratory pathogen infections. Conclusion The RT-LAMP assay offers a rapid, sensitive and straightforward detection for SARS-CoV-2 infection, which could aid the expansion of COVID-19 testing in the public domain and hospitals.
has issue date	2020-05-23(xsd:dateTime)
bibo:doi	10.1101/2020.05.20.20108530
has license	medrxiv
sha1sum (hex)	63a17740a40da7b04903d973531a06f43ae458bb
schema:url	https://doi.org/10.1101/2020.05.20.20108530
resource representing a document's title	Development and clinical application of a rapid and sensitive loop-mediated isothermalamplification test for SARS-CoV-2 infection
resource representing a document's body	covid:63a17740a40da7b04903d973531a06f43ae458bb#body_text
is schema:about of	named entity 'sensitive' named entity 'TO DETECT' named entity 'NOVEL' covid:arg/63a17740a40da7b04903d973531a06f43ae458bb named entity 'timely' named entity 'containment' named entity 'SARS-CoV-2' named entity 'assay' named entity 'treatment of patients' named entity 'loop-mediated isothermal amplification' named entity 'infection' named entity 'preprint' named entity 'RNA' named entity 'SARS-CoV-2' named entity 'viral RNA' named entity 'cross-contamination' named entity 'SARS-CoV-2' named entity 'General Hospital' named entity 'nasopharyngeal swabs' named entity 'next-generation sequencing' named entity 'upper respiratory' named entity 'NGS' named entity 'preprint' named entity 'medRxiv' named entity 'relapse' named entity 'MERS' named entity 'laboratory testing' named entity 'preprint' named entity 'medRxiv' named entity 'PCR' named entity 'SARS-CoV-2' named entity 'Illumina' named entity 'confidence intervals' named entity 'cross-reactivity' named entity 'COVID' named entity 'viral load' named entity 'COVID' named entity 'COVID' named entity 'preprint' named entity 'preprint' named entity 'expectoration' named entity 'medRxiv' named entity 'SARS-CoV-2' named entity 'RNA' named entity 'preprint' named entity 'NGS' named entity 'positive reaction' named entity 'preprint' named entity 'PCR' named entity 'RT-qPCR' named entity 'clean room' named entity 'clinical samples' named entity 'CDC' named entity 'COVID' named entity 'pathogens' named entity 'RNA' named entity 'Shanghai' named entity 'medRxiv' named entity 'virus' named entity 'gene' named entity 'COVID' named entity 'CC-BY-NC-ND 4.0' named entity 'RNA' named entity 'SPAdes' named entity 'reverse transcriptase' named entity 'LOD' named entity 'Guangdong' named entity 'preprint' named entity 'RT-qPCR' named entity 'laboratory tests'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2025 OpenLink Software