About: Purification of infectious bronchitis coronavirus by Sephacryl S-1000 gel chromatography

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Purification of infectious bronchitis coronavirus by Sephacryl S-1000 gel chromatography
Creator	Nagano, H Ohta, S Yagyu, K
Source	Elsevier; Medline; PMC
abstract	Abstract A procedure was developed to purify infectious bronchitis virus (IBV) by gel chromatography (GC) with a Sephacryl S-1000 column. Virus samples concentrated by centrifugation were applied to a Sephacryl S-1000 column and eluted by 0.02 M phosphate buffer (pH 7.2) containing 0.15 M NaCl. Virus particles were recovered mainly in the first peak. Purity of the samples was evaluated by both sodium dodecyl sulfate-polyacrylamide gel electrophoresis and electron microscopy. Using electron microscopy, it was found that there were more spike-rich particles in the virus samples purified by GC than in those purified by sucrose density gradient centrifugation (SDGC). In addition, the hemagglutination unit [log10 (infectivity titer/hemagglutination titer)] of GC-purified virus samples was ∼ 10 times lower than that of SDGC-purified virus samples. These results indicate that Sephacryl S-1000 gel chromatography is useful for purification of IBV.
has issue date	1989-12-31(xsd:dateTime)
bibo:doi	10.1016/0378-1135(89)90023-0
bibo:pmid	2558440
has license	els-covid
sha1sum (hex)	66aced2261cbe68a5e2d55c5229b9b45893ee699
schema:url	https://doi.org/10.1016/0378-1135%2889%2990023-0
resource representing a document's title	Purification of infectious bronchitis coronavirus by Sephacryl S-1000 gel chromatography
has PubMed Central identifier	PMC7117437
has PubMed identifier	2558440
schema:publication	Veterinary Microbiology
resource representing a document's body	covid:66aced2261cbe68a5e2d55c5229b9b45893ee699#body_text
is schema:about of	named entity 'density gradient centrifugation' named entity 'hemagglutination' named entity 'concentrated' named entity 'titer' named entity 'column' named entity 'gel' named entity 'purified' named entity 'Coronavirus' named entity 'PURIFIED' named entity 'SDGC' named entity 'RESULTS' named entity 'USING' named entity 'NAC1' named entity 'VET' named entity 'PURITY' named entity 'PARTICLES' named entity 'VIRUS PARTICLES' named entity 'SAMPLES' named entity 'CORONAVIRUS' named entity 'VIRUS' named entity 'ADDITION' named entity 'TITER' named entity 'SODIUM DODECYL SULFATE-POLYACRYLAMIDE GEL ELECTROPHORESIS' named entity '1000' named entity 'USEFUL' named entity 'RECOVERED' named entity 'CONCENTRATED' covid:arg/66aced2261cbe68a5e2d55c5229b9b45893ee699 named entity 'CONTAINING' named entity '0.02' named entity 'EVALUATED' named entity 'SPIKE' named entity 'RICH' named entity 'THESE' named entity 'PEAK' named entity 'TIMES' named entity 'UNIT' named entity 'PROCEDURE' named entity 'GEL CHROMATOGRAPHY' named entity 'INFECTIOUS BRONCHITIS VIRUS' named entity '1000' named entity 'APPLIED' named entity 'HEMAGGLUTINATION' named entity 'GEL CHROMATOGRAPHY' named entity 'PURIFICATION' named entity 'H.%' named entity 'CORONAVIRUS' named entity 'SUCROSE DENSITY GRADIENT CENTRIFUGATION' named entity 'INFECTIOUS BRONCHITIS' named entity 'PURIFICATION' named entity '0.15' named entity 'FOUND' named entity 'INFECTIOUS BRONCHITIS' named entity 'CENTRIFUGATION' named entity 'PHOSPHATE BUFFER' named entity '7.2' named entity 'ELECTRON MICROSCOPY' named entity 'COLUMN' named entity 'LOWER' named entity 'sucrose' named entity 'gel' named entity 'purification' named entity 'virus' named entity 'virus' named entity 'virus' named entity 'dodecyl' named entity 'phosphate' named entity 'eluted' named entity 'gel chromatography' named entity 'gel chromatography' named entity 'centrifugation' named entity 'infectious bronchitis virus' named entity 'Virus' named entity 'polyacrylamide gel electrophoresis' named entity 'titer' named entity 'infectious bronchitis' named entity 'IBV' named entity 'evaluated' named entity 'Virus'

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