About: Enhancement of wildlife disease surveillance using multiplex quantitative PCR: development of qPCR assays for major pathogens in UK squirrel populations

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Enhancement of wildlife disease surveillance using multiplex quantitative PCR: development of qPCR assays for major pathogens in UK squirrel populations Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Enhancement of wildlife disease surveillance using multiplex quantitative PCR: development of qPCR assays for major pathogens in UK squirrel populations
Creator	Chantrey, Julian Jones, David Begon, Michael Dale, Timothy Everest, David Pounder, Kieran Watts, Phillip
source	Medline; PMC
abstract	Rapid development in polymerase chain reaction (PCR) technology has revolutionised the speed and accuracy of many diagnostic assays. However, comparatively few wildlife epidemiological studies use quantitative PCR (qPCR) for pathogen detection, even fewer employ an internal control, to ensure confidence in negative results, and PCR’s ability to multiplex and therefore detect several targets in a single reaction is underutilised. Here, we describe the development of two multiplex qPCR assays for the red and grey squirrel that detect the pathogens squirrelpox virus (SQPV) and adenovirus in squirrels (SADV), both of which cause mortality in the red squirrel. Both assays use a section of the squirrel phosphoglycerate kinase gene as an endogenous internal control that identifies and compensates for both, inadequate sampling or PCR inhibition. Tests on infected squirrel tissue demonstrate that simple swab samples (particularly from distal antebrachial skin) are sufficient to detect and identify the relative quantity of SQPV DNA in both squirrel species, while rectal swabs and blood cell pellets can be used to reliably indicate SADV infection. These assays are sensitive and specific with an endogenous internal control providing confidence in negative results and allowing comparison across laboratories. Using such assays should prove advantageous in wildlife studies with limited resources while allowing the maximum data yield. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s10344-016-1031-z) contains supplementary material, which is available to authorized users.
has issue date	2016-07-28(xsd:dateTime)
bibo:doi	10.1007/s10344-016-1031-z
bibo:pmid	32214943
has license	cc-by
sha1sum (hex)	66db6c73e03fd309112101100b449e0464d81aa0
schema:url	https://doi.org/10.1007/s10344-016-1031-z
resource representing a document's title	Enhancement of wildlife disease surveillance using multiplex quantitative PCR: development of qPCR assays for major pathogens in UK squirrel populations
has PubMed Central identifier	PMC7088385
has PubMed identifier	32214943
schema:publication	Eur
resource representing a document's body	covid:66db6c73e03fd309112101100b449e0464d81aa0#body_text
is schema:about of	named entity 'MULTIPLEX' named entity 'detect' named entity 'qPCR' named entity 'red squirrel' named entity 'relative' named entity 'quantitative PCR' named entity 'POPULATIONS' named entity 'DEVELOPMENT' named entity 'USING' named entity 'STUDIES' named entity 'SECTION' named entity 'ENDOGENOUS' named entity 'DNA' named entity 'CONTROL' named entity 'SWAB' named entity 'NEGATIVE RESULTS' named entity 'PHOSPHOGLYCERATE KINASE' named entity 'USE' named entity 'CAUSE' named entity 'ENSURE' named entity 'USED' named entity 'CONFIDENCE' named entity 'MORTALITY' named entity 'TISSUE' named entity 'QPCR ASSAYS' named entity 'DATA' named entity 'EPIDEMIOLOGICAL STUDIES' named entity 'WILDLIFE' named entity 'EMPLOY' named entity 'SWABS' named entity 'RECTAL' named entity 'ADENOVIRUS' named entity 'RED SQUIRREL' named entity 'ACROSS' named entity 'COMPARISON' named entity 'SPECIFIC' named entity 'LIMITED' named entity 'SUFFICIENT' named entity 'PATHOGEN DETECTION' named entity 'INFECTED' named entity 'POLYMERASE CHAIN REACTION' named entity 'SAMPLES' named entity 'TECHNOLOGY' named entity 'PELLETS' named entity 'RED' named entity 'PCR' named entity 'TO DETECT' named entity 'PATHOGENS' named entity 'DISTAL' named entity 'KINASE GENE' named entity 'YIELD' named entity 'DETECT' named entity 'DISEASE SURVEILLANCE' named entity 'SAMPLING' named entity 'BLOOD CELL' named entity 'ALLOWING' named entity 'MULTIPLEX' named entity 'SIMPLE' named entity 'PATHOGENS' named entity 'USING' named entity 'QUANTITATIVE PCR' named entity 'MAJOR' named entity 'SQUIRREL' named entity 'ENHANCEMENT' named entity 'REACTION' named entity 'LABORATORIES' named entity 'SINGLE' named entity 'INFECTION' named entity 'HERE' named entity 'SKIN' named entity 'RESOURCES' named entity 'SQUIRRELPOX VIRUS' named entity 'PROVIDING' named entity 'ACCURACY' named entity 'INADEQUATE '

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2025 OpenLink Software