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About:
Identification of a Novel Linear B-Cell Epitope on the Nucleocapsid Protein of Porcine Deltacoronavirus
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research paper
schema:ScholarlyArticle
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Academic Article
research paper
schema:ScholarlyArticle
isDefinedBy
Covid-on-the-Web dataset
has title
Identification of a Novel Linear B-Cell Epitope on the Nucleocapsid Protein of Porcine Deltacoronavirus
Creator
Huang, Xiaobo
Chen, Rui
Cao, Sanjie
Fu, Jiayu
Hu, Jingfei
Ma, Xiaoping
Qu, Huan
Wen, Xintian
Wen, Yiping
Wu, Rui
Zhao, Qin
Zhao, Yujia
Source
Medline; PMC
abstract
Porcine deltacoronavirus (PDCoV), first identified in 2012, is a swine enteropathogen now found in many countries. The nucleocapsid (N) protein, a core component of PDCoV, is essential for virus replication and is a significant candidate in the development of diagnostics for PDCoV. In this study, monoclonal antibodies (mAbs) were generated and tested for reactivity with three truncations of the full protein (N1, N2, N3) that contained partial overlaps; of the five monoclonals chosen tested, each reacted with only the N3 truncation. The antibody designated 4E88 had highest binding affinity with the N protein and was chosen for in-depth examination. The 4E88 epitope was located to amino acids 308-AKPKQQKKPKK-318 by testing the 4E88 monoclonal for reactivity with a series of N3 truncations, then the minimal epitope, 309-KPKQQKKPK-317 (designated EP-4E88), was pinpointed by testing the 4E88 monoclonal for reactivity with a series of synthetic peptides of this region. Homology analysis showed that the EP-4E88 sequence is highly conserved among PDCoV strains, and also shares high similarity with sparrow coronavirus (HKU17), Asian leopard cat coronavirus (ALCCoV), quail coronavirus (UAE-HKU30), and sparrow deltacoronavirus (SpDCoV). Of note, the PDCoV EP-4E88 sequence shared very low similarity (<22.2%) with other porcine coronaviruses (PEDV, TGEV, PRCV, SADS-CoV, PHEV), demonstrating that it is an epitope that can be used for distinguishing PDCoV and other porcine coronavirus. 3D structural analysis revealed that amino acids of EP-4E88 were in close proximity and may be exposed on the surface of the N protein.
has issue date
2020-01-19
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xsd:dateTime
)
bibo:doi
10.3390/ijms21020648
bibo:pmid
31963776
has license
cc-by
sha1sum (hex)
67f9fc7574738473d4155929c1637f1e7c36afb2
schema:url
https://doi.org/10.3390/ijms21020648
resource representing a document's title
Identification of a Novel Linear B-Cell Epitope on the Nucleocapsid Protein of Porcine Deltacoronavirus
has PubMed Central identifier
PMC7013544
has PubMed identifier
31963776
schema:publication
Int J Mol Sci
resource representing a document's body
covid:67f9fc7574738473d4155929c1637f1e7c36afb2#body_text
is
schema:about
of
named entity 'Porcine'
named entity 'protein'
named entity 'shared'
named entity 'reacted'
named entity 'highly'
named entity 'B-Cell'
covid:arg/67f9fc7574738473d4155929c1637f1e7c36afb2
named entity 'protein'
named entity 'swine'
named entity 'similarity'
named entity 'located'
named entity 'Asian leopard cat'
named entity 'structural analysis'
named entity 'series'
named entity 'similarity'
named entity 'contained'
named entity 'peptides'
named entity 'development'
named entity 'virus'
named entity 'coronavirus'
named entity 'truncation'
named entity 'Porcine'
named entity 'coronaviruses'
named entity 'Porcine'
named entity 'epitope'
named entity 'nucleocapsid'
named entity 'PHEV'
named entity 'synthetic peptides'
named entity 'structural analysis'
named entity 'Deltacoronavirus'
named entity 'denatured'
named entity 'PEDV'
named entity 'porcine'
named entity 'immunogen'
named entity 'Hybridomas'
named entity 'ascites'
named entity 'mAb'
named entity 'antibody'
named entity 'sequence identity'
named entity 'PBS'
named entity 'Canada'
named entity 'hybridomas'
named entity 'epitope'
named entity 'bar graph'
named entity 'Bio-Rad'
named entity 'mAb'
named entity 'TGEV'
named entity 'PEDV'
named entity 'recombinant'
named entity 'protein'
named entity 'primary antibody'
named entity 'protein'
named entity 'transmissible gastroenteritis virus'
named entity '1:500'
named entity 'Wuhan'
named entity 'one-way ANOVA'
named entity 'epitope'
named entity 'alphacoronavirus'
named entity 'denatured'
named entity 'H.Q.'
named entity 'coronaviruses'
named entity 'epitope'
named entity 'Beijing'
named entity 'denatured'
named entity 'PEDV'
named entity 'genome'
named entity 'TGEV'
named entity 'coronavirus'
named entity 'porcine'
named entity 'Hybridomas'
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