About: Heterologous viral RNA export elements improve expression of severe acute respiratory syndrome (SARS) coronavirus spike protein and protective efficacy of DNA vaccines against SARS

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About: Heterologous viral RNA export elements improve expression of severe acute respiratory syndrome (SARS) coronavirus spike protein and protective efficacy of DNA vaccines against SARS Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Heterologous viral RNA export elements improve expression of severe acute respiratory syndrome (SARS) coronavirus spike protein and protective efficacy of DNA vaccines against SARS
Creator	Callendret, Benoît Escriou, Nicolas Lorin, Valérie Marianneau, Philippe Betton, Jean-Michel Charneau, Pierre Contamin, Hugues Van Der Werf, Sylvie
source	Elsevier; Medline; PMC
abstract	Abstract The SARS-CoV spike glycoprotein (S) is the main target of the protective immune response in humans and animal models of SARS. Here, we demonstrated that efficient expression of S from the wild-type spike gene in cultured cells required the use of improved plasmid vectors containing donor and acceptor splice sites, as well as heterologous viral RNA export elements, such as the CTE of Mazon-Pfizer monkey virus or the PRE of Woodchuck hepatitis virus (WPRE). The presence of both splice sites and WPRE markedly improved the immunogenicity of S-based DNA vaccines against SARS. Upon immunization of mice with low doses (2 μg) of naked DNA, only intron and WPRE-containing vectors could induce neutralizing anti-S antibodies and provide protection against challenge with SARS-CoV. Our observations are likely to be useful for the construction of plasmid and viral vectors designed for optimal expression of intronless genes derived from cytoplasmic RNA viruses.
has issue date	2007-07-05(xsd:dateTime)
bibo:doi	10.1016/j.virol.2007.01.012
bibo:pmid	17331558
has license	els-covid
sha1sum (hex)	68c3b9a12cb7f00790bdcfe7e32b767f13361f76
schema:url	https://doi.org/10.1016/j.virol.2007.01.012
resource representing a document's title	Heterologous viral RNA export elements improve expression of severe acute respiratory syndrome (SARS) coronavirus spike protein and protective efficacy of DNA vaccines against SARS
has PubMed Central identifier	PMC7103356
has PubMed identifier	17331558
schema:publication	Virology
resource representing a document's body	covid:68c3b9a12cb7f00790bdcfe7e32b767f13361f76#body_text
is schema:about of	named entity 'cytoplasmic' named entity 'DNA vaccines' named entity 'efficacy' named entity 'VIRAL RNA' named entity 'EXPORT' named entity 'SPIKE PROTEIN' named entity 'SARS-COV' named entity 'PROVIDE' named entity 'ANIMAL MODELS ' named entity 'GENES' named entity 'DOSES' named entity 'WOODCHUCK HEPATITIS VIRUS' named entity 'MONKEY' named entity 'PLASMID' named entity 'USEFUL' named entity 'SARS' named entity 'IMMUNOGENICITY' named entity 'MICE' named entity 'DNA VACCINES' named entity 'CONTAINING' named entity 'HUMANS' named entity 'LOW' named entity 'LIKELY' named entity 'ELEMENTS' named entity 'IMPROVED' named entity 'WILD-TYPE' named entity 'IMMUNE RESPONSE' named entity 'CHALLENGE' named entity 'CONSTRUCTION' named entity '28S' named entity 'GENE' named entity 'VIRUS' named entity 'USE OF' named entity 'DONOR' named entity 'EFFICIENT' named entity 'CORONAVIRUS' named entity 'EFFICACY' named entity 'ANTIBODIES' named entity 'VECTORS' named entity 'EXPRESSION' named entity 'CULTURED CELLS' named entity 'EXPORT' named entity 'OBSERVATIONS' named entity 'VIRAL RNA' named entity 'PRE' named entity 'IMMUNIZATION' named entity 'BCG' covid:arg/68c3b9a12cb7f00790bdcfe7e32b767f13361f76 named entity 'MARKEDLY' named entity '282' named entity 'PLASMID VECTORS' named entity 'INTRON' named entity 'NAKED DNA' named entity 'SARS' named entity 'SEVERE ACUTE RESPIRATORY SYNDROME' named entity 'EXPRESSION' named entity 'PROTEIN ' named entity 'DNA VACCINES' named entity 'ELEMENTS' named entity 'CYTOPLASMIC RNA' named entity 'INDUCE' named entity 'OPTIMAL' named entity 'OUR' named entity 'PRESENCE OF' named entity 'VIRAL VECTORS' named entity 'SPLICE SITES' named entity 'TARGET' named entity 'PFIZER' named entity 'DERIVED' named entity 'HERE' named entity 'BASED' named entity 'RNA VIRUSES' named entity 'ACCEPTOR' named entity 'DEMONSTRATED'

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