About: Selection of Suitable Reference Genes for Normalization of Quantitative Real-Time Polymerase Chain Reaction in Human Cartilage Endplate of the Lumbar Spine

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About: Selection of Suitable Reference Genes for Normalization of Quantitative Real-Time Polymerase Chain Reaction in Human Cartilage Endplate of the Lumbar Spine Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Selection of Suitable Reference Genes for Normalization of Quantitative Real-Time Polymerase Chain Reaction in Human Cartilage Endplate of the Lumbar Spine
Creator	Wang, P Chen, J-Y Xia, J-F Zhang, Z-J
Source	PMC
abstract	The quantitative real-time polymerase chain reaction (qRT-PCR) is one of the most widely used methods to study gene expression profiles, and it requires appropriate normalization for accurate and reliable results. Although several genes are commonly used as reference genes (such as GAPDH, ACTB, and 18S rRNA), they are also regulated and can be expressed at varying levels. In this study, we evaluated twelve well-known reference genes to identify the most suitable housekeeping gene for normalization of qRT-PCR in human lumbar vertebral endplate with Modic changes, by using the geNorm, NormFinder, and BestKeeper algorithms. Our results showed that the rarely-used SDHA was the most stable single reference gene, and a combination of three, SDHA, B2M, and LDHA, was the most suitable gene set for normalization in all samples. In addition, the commonly-used genes, GAPDH, ACTB and 18S rRNA, were all inappropriate as internal standards. The rankings of reference genes for the three types of Modic change differed, although SDHA and RPL13A uniformly ranked in the first and last position, respectively. Further simulated expression analysis validated that the arbitrary use of a reference gene could lead to the misinterpretation of data. Our study confirmed the necessity of exploring the expression stability of potential reference genes in each specific tissue and experimental situation before quantitative evaluation of gene expression by qRT-PCR.
has issue date	2014-02-18(xsd:dateTime)
bibo:doi	10.1371/journal.pone.0088892
bibo:pmid	24558443
has license	cc-by
sha1sum (hex)	6a2386de99a966c8755732f196ba4c2cc0dda2f2
schema:url	https://doi.org/10.1371/journal.pone.0088892
resource representing a document's title	Selection of Suitable Reference Genes for Normalization of Quantitative Real-Time Polymerase Chain Reaction in Human Cartilage Endplate of the Lumbar Spine
has PubMed Central identifier	PMC3928306
has PubMed identifier	24558443
schema:publication	PLoS One
resource representing a document's body	covid:6a2386de99a966c8755732f196ba4c2cc0dda2f2#body_text
is schema:about of	named entity 'LUMBAR SPINE' named entity 'tissue' named entity 'qRT-PCR' named entity 'gene' named entity 'commonly' named entity 'Further' named entity 'types' named entity 'Although' named entity 'GENES' named entity 'RESULTS' named entity 'EXPRESSED' named entity 'COMBINATION' named entity 'HOUSEKEEPING GENE' named entity 'RANKINGS' named entity 'B2M' named entity 'INTERNAL' named entity 'SDHA' named entity 'EXPRESSION ANALYSIS' named entity 'REFERENCE GENE' named entity 'SET' named entity 'ADDITION' named entity 'LEVELS' named entity 'PCR' named entity '18S RRNA' named entity 'RPL13A' named entity 'NECESSITY' named entity 'VARYING' named entity 'POTENTIAL' named entity 'STABILITY' named entity 'SUITABLE' named entity 'USE OF' named entity 'CONFIRMED' named entity 'USING' named entity 'EVALUATED' named entity 'STABLE' named entity 'GENE' named entity 'LEAD' named entity 'LDHA' named entity 'HUMAN' named entity 'ACCURATE' named entity 'SPECIFIC' named entity 'QRT-PCR' named entity 'CHANGE' named entity 'SITUATION' named entity 'ONE OF' named entity 'RARELY' named entity 'GENE EXPRESSION PROFILES' named entity 'STANDARDS' named entity 'QUANTITATIVE EVALUATION' named entity 'INAPPROPRIATE' named entity 'QUANTITATIVE REAL-TIME POLYMERASE CHAIN REACTION' named entity 'APPROPRIATE' named entity 'SUITABLE' named entity 'POSITION' named entity 'ALGORITHMS' named entity 'ARBITRARY' named entity 'REFERENCE' named entity 'QUANTITATIVE REAL-TIME POLYMERASE CHAIN REACTION' named entity 'ENDPLATE' named entity 'GENE EXPRESSION' named entity 'SELECTION' named entity 'NORMALIZATION' named entity 'DATA' named entity 'GENES' named entity 'TO IDENTIFY' named entity 'STUDY' named entity 'TISSUE' named entity 'LUMBAR' named entity 'USED' named entity 'VERTEBRAL ENDPLATE' named entity 'REFERENCE' named entity 'RELIABLE' named entity 'KNOWN' named entity 'GAPDH' named entity 'TYPES' named entity 'HUMAN CARTILAGE' named entity 'VALIDATED' named entity 'EXPERIMENTAL'

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