About: Isolation and Characterization of a Novel Gammaherpesvirus from a Microbat Cell Line

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Isolation and Characterization of a Novel Gammaherpesvirus from a Microbat Cell Line
Creator	Puri, Vinita Attie, Oliver Jayaprakash, Anitha Sachidanandam, Ravi Shrivastava, Susmita Tsibane, Tshidi Basler, Christopher Dilley, Kari Geisbert, Thomas Mire, Chad Shabman, Reed Stockwell, Timothy
topic	covid:6b58f76c98afb6435969ef4d9f9a063058265b98#this
Source	Medline; PMC
abstract	While employing deep sequencing and de novo assembly to characterize the mRNA transcript profile of a cell line derived from the microbat Myotis velifer incautus, we serendipitously identified mRNAs encoding proteins with a high level of identity to herpesviruses. A majority were closely related to proteins of equine herpesvirus 2 (EHV-2), a horse gammaherpesvirus. We demonstrated by electron microscopy the presence of herpesvirus-like particles in the microbat cells. Passage of supernatants from microbat cells to Vero cells resulted in syncytium formation, and expression of viral genes and amplification of viral DNA were demonstrated by quantitative PCR. Susceptibility of human cell lines to productive infection was also demonstrated. Next-generation sequencing and de novo assembly of the viral genome from supernatants from Vero cells yielded a single contig of approximately 130 kb with at least 77 open reading frames (ORFs), predicted microRNAs (miRNAs), and a gammaherpesvirus genomic organization. Phylogenic analysis of the envelope glycoprotein (gB) and DNA polymerase (POLD1) revealed similarity to multiple gammaherpesviruses, including those from as-yet-uncultured viruses of the Rhadinovirus genus that were obtained by deep sequencing of bat tissues. Moreover, the assembled genome revealed ORFs that share little or no homology to known ORFs in EHV-2 but are similar to accessory proteins of other gammaherpesviruses. Some also have striking homology to predicted Myotis bat proteins. Cumulatively, this study provides the first isolation and characterization of a replication-competent bat gammaherpesvirus. IMPORTANCE Bats are of significant interest as reservoirs for zoonotic viral pathogens; however, tools to dissect bat-virus interactions are limited in availability. This study serendipitously identified, in an established bat cell line, a fully replication-competent gammaherpesvirus; determined the complete genome sequence of the virus; and generated a viral transcript map. This virus can replicate in select human and nonhuman primate cell lines. However, analyses of viral sequences support a bat origin for this virus; we therefore refer to the virus as bat gammaherpesvirus 8 (BGHV8). The viral genome contains unique open reading frames that likely encode modulators of bat innate and adaptive immune signaling pathways and expresses viral miRNAs. The virus and its gene products should provide a unique tool to dissect both bat and gammaherpesvirus biology.
has issue date	2016-02-17(xsd:dateTime)
bibo:doi	10.1128/msphere.00070-15
bibo:pmid	27303702
has license	cc-by
sha1sum (hex)	6b58f76c98afb6435969ef4d9f9a063058265b98
schema:url	https://doi.org/10.1128/msphere.00070-15
resource representing a document's title	Isolation and Characterization of a Novel Gammaherpesvirus from a Microbat Cell Line
has PubMed Central identifier	PMC4863610
has PubMed identifier	27303702
schema:publication	mSphere
resource representing a document's body	covid:6b58f76c98afb6435969ef4d9f9a063058265b98#body_text
is http://vocab.deri.ie/void#inDataset of	https://covidontheweb.inria.fr:4443/about/id/http/ns.inria.fr/covid19/6b58f76c98afb6435969ef4d9f9a063058265b98
is schema:about of	named entity 'microRNAs' named entity 'formation' named entity 'While' named entity 'mRNAs' named entity 'infection' named entity 'amplification' named entity 'gammaherpesvirus' named entity 'VIRAL DNA' named entity 'MICRORNAS' named entity 'HOMOLOGY' named entity 'IDENTITY' named entity 'ASSEMBLY' named entity 'AMPLIFICATION' named entity 'HERPESVIRUSES' named entity 'DE NOVO' named entity 'ELECTRON MICROSCOPY' named entity 'PROTEINS' named entity 'ENCODING' named entity 'RHADINOVIRUS' named entity 'EXPRESSION' named entity 'EQUINE HERPESVIRUS 2' named entity 'MYOTIS VELIFER' named entity 'ORGANIZATION' named entity 'EHV-2' named entity 'VIRAL GENOME' named entity 'ACCESSORY' named entity 'PARTICLES' named entity 'SIMILARITY' named entity 'LIKE' named entity 'DERIVED' named entity 'TRANSCRIPT' named entity 'ISOLATION' named entity 'MULTIPLE' named entity 'HUMAN CELL LINES' named entity 'CONTIG' named entity 'VERO CELLS' named entity 'CHARACTERIZATION' named entity 'NEW' named entity 'REPORT' named entity 'CELL LINE' named entity 'VIRUSES' named entity 'GAMMAHERPESVIRUS' named entity 'BUT' named entity 'SUSCEPTIBILITY' named entity 'VIRAL GENES' named entity 'BAT' named entity 'HERPESVIRUS' named entity 'GENUS' named entity 'INCLUDING' named entity 'SYNCYTIUM FORMATION' named entity 'HAVE' named entity 'STRIKING' named entity 'CHARACTERIZATION' named entity 'TISSUES' named entity 'GENOMIC' named entity 'ENVELOPE GLYCOPROTEIN' named entity 'EMPLOYING' named entity 'GENOME' named entity 'HORSE' named entity 'PASSAGE' named entity 'PROVIDES' named entity 'REVEALED' named entity 'INFECTION' named entity 'PRODUCTIVE' named entity 'CELLS' named entity 'CELL LINE' named entity 'LITTLE' named entity 'NEXT-GENERATION SEQUENCING'

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