About: MicroRNA transcriptome analysis of porcine vital organ responses to immunosuppressive porcine cytomegalovirus infection

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About: MicroRNA transcriptome analysis of porcine vital organ responses to immunosuppressive porcine cytomegalovirus infection Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	MicroRNA transcriptome analysis of porcine vital organ responses to immunosuppressive porcine cytomegalovirus infection
Creator	Liao, Shan Liu, Xiao Wei, Haoche Xu, Zhiwen Ye, Jianheng Zhu, Ling
Source	Medline; PMC
abstract	BACKGROUND: Porcine cytomegalovirus (PCMV) is an immunosuppressive virus that mainly inhibits T-lymphocyte and macrophage immune functions; it has significantly damaged the farming industry. Although recent studies have shown that miRNAs play important roles in immune responses, the regulatory mechanisms of miRNAs during immunosuppressive virus infection remain unclear. METHODS: In this study, porcine small-RNA transcriptomes of PCMV-infected and uninfected vital organs were first characterised by high-throughput sequencing. miRDeep2 software was used to predict novel pig-encoded miRNAs. To verify the accuracy of the high-throughput sequencing results, stem-loop qRT-PCR was performed on 12 significantly DE miRNAs. The physical and functional interactions between the immune-related target genes of the DE miRNAs in PCMV-infected organs were analysed using the STRING database. RESULTS: In total, 306 annotated and 295 novel miRNAs were identified from PCMV-infected and uninfected porcine organs, respectively, through alignment with known Sus scrofa pre-miRNAs. Overall, 92, 107, 95, 77 and 111 miRNAs were significantly differentially expressed in lung, liver, spleen, kidney and thymus after PCMV infection, respectively. According to Gene Ontology enrichment analysis, target genes of the differentially expressed miRNAs associated with immune system processes, regulation of biological processes and metabolic processes were enriched in every sample. Integrated expression analysis of the differentially expressed miRNAs and their target mRNAs in PCMV-infected thymus showed that the significant differential expression of specific miRNAs under the pressure of PCMV infection in central immune organs interfered with the expression of genes involved in important immune-related signalling pathways, thus promoting the viral infection. CONCLUSIONS: This is the first comprehensive analysis of the responses of host small-RNA transcriptomes to PCMV infection in vital porcine organs. It provides new insights into the regulatory mechanisms of miRNAs during infection by immunosuppressive viruses. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12985-018-0922-x) contains supplementary material, which is available to authorized users.
has issue date	2018-01-18(xsd:dateTime)
bibo:doi	10.1186/s12985-018-0922-x
bibo:pmid	29347945
has license	cc-by
sha1sum (hex)	6cd8cfb4dd10db30a8bbd630da2e8c15ee4e4956
schema:url	https://doi.org/10.1186/s12985-018-0922-x
resource representing a document's title	MicroRNA transcriptome analysis of porcine vital organ responses to immunosuppressive porcine cytomegalovirus infection
has PubMed Central identifier	PMC5774105
has PubMed identifier	29347945
schema:publication	Virol J
resource representing a document's body	covid:6cd8cfb4dd10db30a8bbd630da2e8c15ee4e4956#body_text
is schema:about of	named entity 'VITAL' named entity 'industry' named entity 'REGULATORY' named entity 'BACKGROUND' named entity 'MECHANISMS' named entity 'ROLES' named entity 'VIRUS' named entity 'IMMUNE' named entity 'IMMUNOSUPPRESSIVE' named entity 'MACROPHAGE' named entity 'TRANSCRIPTOME ANALYSIS' named entity 'UNCLEAR' named entity 'IMPORTANT' named entity 'T-LYMPHOCYTE' named entity 'PLAY' named entity 'FARMING' named entity 'CYTOMEGALOVIRUS INFECTION' named entity 'IMMUNOSUPPRESSIVE' named entity 'PORCINE CYTOMEGALOVIRUS' named entity 'RESPONSES' named entity 'RECENT' named entity 'VIRUS INFECTION' named entity 'IMMUNE RESPONSES' named entity 'ORGAN' named entity 'INDUSTRY' named entity 'PORCINE CYTOMEGALOVIRUS' named entity 'PORCINE' named entity 'MICRORNA' named entity 'MIRNAS' named entity 'HAVE' named entity 'FUNCTIONS' named entity 'STUDIES' named entity 'DAMAGED' covid:arg/6cd8cfb4dd10db30a8bbd630da2e8c15ee4e4956 named entity 'infection' named entity 'significant impact' named entity 'miRNAs' named entity 'p-value' named entity 'differentially expressed' named entity 'T-lymphocyte' named entity 'remain' named entity 'macrophage' named entity 'miRNAs' named entity 'immune functions' named entity 'virus' named entity 'miRNAs' named entity 'transcriptome analysis' named entity 'cytomegalovirus infection' named entity 'porcine' named entity 'macrophage' named entity 'signalling pathways' named entity 'coinfection' named entity 'cytokine' named entity 'pig' named entity 'piglet' named entity 'stem-loop' named entity 'Ensembl' named entity 'high-throughput sequencing' named entity 'sRNA' named entity 'thymus' named entity 'miR-19b' named entity 'ncRNA' named entity 'liver' named entity 'tumor necrosis factor' named entity 'downregulated' named entity 'CD4' named entity 'miRNA' named entity 'miR' named entity 'miRBase' named entity 'miRNA' named entity 'expression profiles' named entity 'viruses' named entity 'differentially expressed' named entity 'lung' named entity 'signalling pathways' named entity 'viral infection'

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