About: Ultrastructural characterization of membranous torovirus replication factories

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Ultrastructural characterization of membranous torovirus replication factories
Creator	Rodríguez, Dolores Rejas, María
source	Medline; PMC
abstract	Plus‐stranded RNA viruses replicate in the cytosol of infected cells, in membrane‐bound replication complexes containing the replicase proteins, the viral RNA and host proteins. The formation of the replication and transcription complexes (RTCs) through the rearrangement of cellular membranes is currently being actively studied for viruses belonging to different viral families. In this work, we identified double‐membrane vesicles (DMVs) in the cytoplasm of cells infected with the equine torovirus Berne virus (BEV), the prototype member of the Torovirus genus (Coronaviridae family, Nidovirales order). Using confocal microscopy and transmission electron microscopy, we observed a close relationship between the RTCs and the DMVs of BEV. The examination of BEV‐infected cells revealed that the replicase proteins colocalize with each other and with newly synthesized RNA and are associated to the membrane rearrangement induced by BEV. However, the double‐stranded RNA, an intermediate of viral replication, is exclusively limited to the interior of DMVs. Our results with BEV resemble those obtained with other related viruses in the Nidovirales order, thus providing new evidence to support the idea that nidoviruses share a common replicative structure based on the DMV arranged clusters.
has issue date	2016-07-05(xsd:dateTime)
bibo:doi	10.1111/cmi.12620
bibo:pmid	27218226
has license	no-cc
sha1sum (hex)	6ce4b3008e4c7a34aa6529bd31c01b41fdeb5f71
schema:url	https://doi.org/10.1111/cmi.12620
resource representing a document's title	Ultrastructural characterization of membranous torovirus replication factories
has PubMed Central identifier	PMC7162420
has PubMed identifier	27218226
schema:publication	Cell Microbiol
resource representing a document's body	covid:6ce4b3008e4c7a34aa6529bd31c01b41fdeb5f71#body_text
is schema:about of	named entity 'prototype' named entity 'replication' named entity 'studied' named entity 'replication' named entity 'CLUSTERS' named entity 'VESICLES' named entity 'MEMBRANES' named entity 'Our' named entity 'cytoplasm' named entity 'replicate' named entity 'formation' named entity 'Nidovirales' named entity 'viruses' named entity 'transmission electron microscopy' named entity 'transcription' named entity 'Berne' named entity 'DMVs' named entity 'DMV' named entity 'equine' named entity 'DMVs' named entity 'ERGIC' named entity 'antibodies' named entity 'transcription' named entity 'organelle' named entity 'viral RNA' named entity 'ORFs' named entity 'DMV' named entity 'messenger RNAs' named entity 'nitrocellulose' named entity 'IBV' named entity 'ERGIC' named entity 'colocalization' named entity 'infection' named entity 'Electron tomography' named entity 'cysteine' named entity 'antibodies' named entity 'DMVs' named entity 'protein' named entity 'viral RNA' named entity 'organelle' named entity 'RdRp' named entity 'glyceraldehyde 3-phosphate dehydrogenase' named entity 'EAV' named entity 'RNA' named entity 'BrU' named entity 'genus' named entity 'virus' named entity 'antibodies' named entity 'EAV' named entity 'OS-9' named entity 'helicase' named entity 'colocalization' named entity 'TEM' named entity 'transmission electron microscopy' named entity 'S1C' named entity 'dsRNA' named entity 'plasmids' named entity 'dsRNA' named entity 'Endoplasmic reticulum' named entity 'protein' named entity 'confocal microscopy' named entity 'DMV' named entity 'epoxy resin' named entity 'EAV' named entity 'TEM' named entity 'DMVs' named entity 'antibodies' named entity 'glycine' named entity 'p53' named entity 'viral RNA' named entity 'Utrecht' named entity 'ribosomal frameshifting' named entity 'FCS' named entity 'DMVs' named entity 'viruses' named entity 'structural proteins' named entity 'rod-shaped' named entity 'cryosectioning' named entity 'nsp' named entity 'dsRNA'

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