About: Ultrasensitive label-free electrochemiluminescence immunosensor based on N-(4-aminobutyl)-N-ethylisoluminol-functionalized graphene composite

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About: Ultrasensitive label-free electrochemiluminescence immunosensor based on N-(4-aminobutyl)-N-ethylisoluminol-functionalized graphene composite Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Ultrasensitive label-free electrochemiluminescence immunosensor based on N-(4-aminobutyl)-N-ethylisoluminol-functionalized graphene composite
Creator	Shen, Wen Cui, Hua Shu, Jiangnan
Source	Medline; PMC
abstract	The electrochemiluminescence (ECL) behavior of N-(4-aminobutyl)-N-ethylisoluminol (ABEI)-functionalized graphene composite (ABEI-GC) modified on an indium tin oxide (ITO) electrode was studied. ABEI-GC exhibited excellent ECL activity. On this basis, a label-free ECL immunosensor was developed for the sensitive detection of human immunoglobulin G (hIgG) by using ABEI-GC as the ECL nano-interface via a layer-by-layer assembly technique. ABEI-GC was first assembled onto an ITO electrode. Positively charged chitosan was then electrostatically adsorbed to the modified electrode. Finally, negatively charged antibody-coated gold nanoparticles were attached to the surface to form the ECL immunosensor. In the presence of hIgG, hIgG was captured by its antibody. In addition, an ECL signal was detected in the presence of H(2)O(2) when a double potential was applied. The ECL immunosensor for the determination of hIgG showed a linear range of 1.0×10(−13)–1.0×10(−8) g/mL with a detection limit of 5.0×10(−14) g/mL. This immunosensor has high sensitivity, wide linearity and good reproducibility. The superior sensitivity of the proposed ECL immunoassay mainly derives from the incorporation of ABEI-GC, which not only improves the ECL intensity, response speed, and stability, but also provides a large specific surface for high levels of protein loading. This work reveals that ABEI-GC is good nano-interface for the construction of ECL biosensors. Our strategy is promising for protein detection and may open up a new avenue for ultrasensitive label-free immunoassays.
has issue date	2015-01-22(xsd:dateTime)
bibo:doi	10.1007/s11426-015-5320-2
bibo:pmid	32215002
has license	no-cc
sha1sum (hex)	6f396dca9b8d5d8e40af2f4aab4c91584ecd2776
schema:url	https://doi.org/10.1007/s11426-015-5320-2
resource representing a document's title	Ultrasensitive label-free electrochemiluminescence immunosensor based on N-(4-aminobutyl)-N-ethylisoluminol-functionalized graphene composite
has PubMed Central identifier	PMC7088698
has PubMed identifier	32215002
schema:publication	Sci China Chem
resource representing a document's body	covid:6f396dca9b8d5d8e40af2f4aab4c91584ecd2776#body_text
is schema:about of	named entity 'ECL' named entity 'presence' named entity 'ECL' named entity 'ECL' named entity 'biosensors' named entity 'immunoglobulin G' named entity 'label-free' named entity 'graphene' named entity 'APPLIED' named entity 'RESPONSE' named entity 'H 2' named entity 'DETECTED' named entity 'ACTIVITY' named entity 'USING' named entity 'STABILITY' named entity 'AVENUE' named entity 'EXCELLENT' named entity 'OUR' named entity 'LEVELS OF PROTEIN' named entity 'LAYER-BY-LAYER ASSEMBLY' named entity 'IMPROVES' named entity 'INTENSITY' named entity 'STRATEGY' named entity 'PRESENCE OF' named entity 'BEHAVIOR' named entity 'PROPOSED' named entity 'POSITIVELY CHARGED' named entity 'WORK' named entity 'SPECIFIC' named entity 'LABEL-FREE' named entity 'CHITOSAN' named entity 'COMPOSITE' named entity 'NEW' named entity 'AD13' named entity 'MODIFIED' named entity 'BASIS' named entity 'ADDITION' named entity 'PROVIDES' named entity 'LINEAR RANGE' named entity 'ELECTROCHEMILUMINESCENCE' named entity 'CHINA' named entity 'CHEMISTRY' named entity 'GRAPHENE' named entity 'SCIENCE' named entity 'OPEN' named entity 'GOLD' named entity 'ITO' named entity 'AD8' named entity 'DETECTION' named entity 'REPRODUCIBILITY' named entity 'BASED' named entity 'SENSITIVE' named entity 'BUT' named entity 'TECHNIQUE' named entity 'HIGH' named entity 'AD14' named entity 'ATTACHED TO' named entity 'NEGATIVELY CHARGED' named entity 'SPEED' named entity 'HIGH SENSITIVITY' named entity 'LARGE' named entity 'NANOPARTICLES' named entity 'IMMUNOASSAY' named entity 'HIGG' named entity 'GOOD' named entity 'DETECTION LIMIT' named entity 'ITS' named entity 'LABEL-FREE' named entity 'BIOSENSORS' named entity 'INDIUM TIN OXIDE' named entity 'IMMUNOASSAYS' named entity '80%' named entity 'COATED' named entity 'DETERMINATION' named entity 'SUPERIOR' named entity 'PROTEIN ' named entity 'PROTEIN DETECTION' named entity '2 O' named entity 'CONSTRUCTION'

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