About: Comparison of SARS‐CoV‐2 detection from nasopharyngeal swab samples by the Roche cobas 6800 SARS‐CoV‐2 test and a laboratory‐developed real‐time RT‐PCR test

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About: Comparison of SARS‐CoV‐2 detection from nasopharyngeal swab samples by the Roche cobas 6800 SARS‐CoV‐2 test and a laboratory‐developed real‐time RT‐PCR test Goto Sponge NotDistinct Permalink

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Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Comparison of SARS‐CoV‐2 detection from nasopharyngeal swab samples by the Roche cobas 6800 SARS‐CoV‐2 test and a laboratory‐developed real‐time RT‐PCR test
Creator	Paniz-Mondolfi, Alberto Nowak, Michael Alicia, \| Carlos Cordon-Cardo, \| Chiu, Numthip Correspondence, Melissa Flores, Vanessa Gitman, Gitman, Melissa Hernandez, Matthew Houldsworth, Jane Nnaemeka Ibeh, \| Pujadas, Elisabet Shiffrin, Biana Sidorenko, Tatyana Sordillo, Emilia Waluszko, Aneta
source	Medline; PMC
abstract	The urgent need to implement and rapidly expand testing for severe acute respiratory syndrome coronavirus‐2 (SARS‐CoV‐2) infection has led to the development of multiple assays. How these tests perform relative to one another is poorly understood. We evaluated the concordance between the Roche Diagnostics cobas 6800 SARS‐CoV‐2 test and a laboratory‐developed test (LDT) real‐time reverse transcription‐polymerase chain reaction based on a modified Centers for Disease Control and Prevention protocol, for the detection of SARS‐CoV‐2 in samples submitted to the Clinical Laboratories of the Mount Sinai Health System. A total of 1006 nasopharyngeal swabs in universal transport medium from persons under investigation were tested for SARS‐CoV‐2 as part of routine clinical care using the cobas SARS‐CoV‐2 test with subsequent evaluation by the LDT. Cycle threshold values were analyzed and interpreted as either positive (“detected” or “presumptive positive”), negative (not detected), inconclusive, or invalid. Statistical analysis was performed using GraphPad Prism 8. The cobas SARS‐CoV‐2 test reported 706 positive and 300 negative results. The LDT reported 640 positive, 323 negative, 34 inconclusive, and 9 invalid results. When excluding inconclusive and invalid results, the overall percent agreement between the two platforms was 95.8%. Cohen's κ coefficient was 0.904 (95% confidence interval, 0.875‐0.933), suggesting almost perfect agreement between both platforms. An overall discordance rate of 4.2% between the two systems may reflect differences in primer sequences, assay limit of detection, or other factors, highlighting the importance of comparing the performance of different testing platforms.
has issue date	2020-05-22(xsd:dateTime)
bibo:doi	10.1002/jmv.25988
bibo:pmid	32383179
has license	no-cc
sha1sum (hex)	6fe5e58d8165785269a6bd6b82698c800d1ad972
schema:url	https://doi.org/10.1002/jmv.25988
resource representing a document's title	Comparison of SARS‐CoV‐2 detection from nasopharyngeal swab samples by the Roche cobas 6800 SARS‐CoV‐2 test and a laboratory‐developed real‐time RT‐PCR test
has PubMed Central identifier	PMC7267546
has PubMed identifier	32383179
schema:publication	J Med Virol
resource representing a document's body	covid:6fe5e58d8165785269a6bd6b82698c800d1ad972#body_text
is schema:about of	named entity 'total' named entity 'testing' named entity 'relative' named entity 'test' named entity 'test' named entity 'platforms' named entity 'SARS-CoV-2' named entity 'subsequent' named entity 'transport medium' named entity 'test' named entity 'TESTING' named entity 'ASSAY' named entity 'DETECTION' named entity 'COEFFICIENT' named entity 'POORLY' named entity 'RNA EXTRACTION' named entity 'CENTERS FOR DISEASE CONTROL AND PREVENTION' named entity 'MULTIPLE' named entity 'INVALID' named entity 'SEVERE ACUTE RESPIRATORY SYNDROME CORONAVIRUS' named entity 'TEST' named entity 'multiple' named entity 'rapidly' named entity 'swabs' named entity 'expand' named entity 'LDT' named entity 'comparing' named entity 'SARS-CoV-2' named entity 'Laboratories' named entity 'positive' named entity 'concordance' named entity 'SARS-CoV-2' named entity 'severe acute respiratory syndrome coronavirus' named entity 'Statistical analysis' named entity 'reverse transcription-polymerase chain reaction' named entity 'clinical care' named entity 'Clinical Laboratories' named entity 'primer' named entity 'SARS-CoV-2' named entity 'SARS-CoV-2' named entity 'cobas' named entity 'Roche' named entity 'nasopharyngeal swab' named entity 'real-time RT-PCR' named entity 'gene' named entity 'internal control' named entity '95% CI' named entity 'SARS-CoV-2' named entity 'SARS-CoV-2' named entity 'ORF1' named entity 'Cohen's κ' named entity 'SARS-CoV-2' named entity 'SARS-CoV-2' named entity 'Cohen's κ' named entity 'LOD' named entity 'La Jolla' named entity 'virus' named entity 'virus' named entity 'RNA genome' named entity 'gold standard' named entity 'SARS-CoV-2' named entity 'GraphPad Prism' named entity 'Sarbecovirus' named entity 'SARS-CoV-2' named entity 'diagnostic tests'

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