About: Validation and application of normalization factors for gene expression studies in rubella virus‐infected cell lines with quantitative real‐time PCR

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Validation and application of normalization factors for gene expression studies in rubella virus‐infected cell lines with quantitative real‐time PCR Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Validation and application of normalization factors for gene expression studies in rubella virus‐infected cell lines with quantitative real‐time PCR
Creator	Claus, C Liebert, U Chey, S
Source	Medline; PMC
abstract	Reference genes are generally employed in real‐time quantitative PCR (RT‐qPCR) experiments to normalize variability between different samples. The aim of this study was to identify and validate appropriate reference genes as internal controls for RT‐qPCR experiments in rubella virus (RV)‐infected Vero and MCF‐7 cell lines using SYBR green fluorescence. The software programs geNorm and NormFinder and the ΔΔC(t) calculation were used to determine the expression stability and thus reliability of nine suitable reference genes. HPRT1 and HUEL, and HUEL and TBP were identified to be most suitable for RT‐qPCR analysis of RV‐infected Vero and MCF‐7 cells, respectively. These genes were used as normalizers for transcriptional activity of selected cellular genes. The results confirm previously published microarray and Northern blot data, particularly on the transcriptional activity of the cyclin‐dependent kinase inhibitor p21 and the nuclear body protein SP100. Furthermore, the mRNA level of the mitochondrial protein p32 is increased in RV‐infected cells. The effect on cellular gene transcription by RV‐infection seems to be cell line‐specific, but genes of central importance for viral life cycle appear to be altered to a similar degree. This study does not only provide an accurate and flexible tool for the quantitative analysis of gene expression patterns in RV‐infected cell lines. It also indicates, that the suitability of a reference gene as normalizer of RT‐qPCR data and the host‐cell response to RV‐infection are strictly cell‐line specific. J. Cell. Biochem. 110: 118–128, 2010. © 2010 Wiley‐Liss, Inc.
has issue date	2010-03-09(xsd:dateTime)
bibo:doi	10.1002/jcb.22518
bibo:pmid	20217898
has license	no-cc
sha1sum (hex)	6fe6587d971d5436d450bc209cb13c798a52e2da
schema:url	https://doi.org/10.1002/jcb.22518
resource representing a document's title	Validation and application of normalization factors for gene expression studies in rubella virus‐infected cell lines with quantitative real‐time PCR
has PubMed Central identifier	PMC7166394
has PubMed identifier	20217898
schema:publication	J Cell Biochem
resource representing a document's body	covid:6fe6587d971d5436d450bc209cb13c798a52e2da#body_text
is schema:about of	named entity 'TOOL' named entity 'HPRT1' named entity 'P32' named entity 'CELL LINES' named entity 'INCREASED' named entity 'infection' named entity 'microarray' named entity 'cell lines' named entity 'reference genes' named entity 'Northern blot' named entity 'normalizer' named entity 'RT-qPCR' named entity 'transcriptional activity' named entity 'fluorescence' named entity 'Rubella Virus' named entity 'breast carcinoma' named entity 'cell-type' named entity 'MCF-7' named entity 'GAPDH' named entity 'analysis of variance' named entity 'RNA' named entity 'MCF-7' named entity 'Reference genes' named entity 'Germany' named entity 'RT-qPCR' named entity 'infection' named entity 'mRNA expression' named entity 'MCF-7' named entity 'FCS' named entity 'protein' named entity 'cell cultures' named entity 'mRNA' named entity 'protein' named entity 'reference genes' named entity 'MCF-7' named entity 'sense and antisense' named entity 'mitochondrial' named entity 'Vero cells' named entity 'MCF-7' named entity 'SP100' named entity 'cell lines' named entity 'gene' named entity 'RT-qPCR' named entity 'gene' named entity 'standard deviation' named entity 'electrophoresis' named entity 'plasmid DNA' named entity 'reference genes' named entity 'subclinical' named entity 'infection' named entity 'Microsoft excel' named entity 'MCF-7' named entity 'A260' named entity 'MCF-7' named entity 'RT-qPCR' named entity 'Eagle's minimum essential medium' named entity 'variable expression' named entity 'mRNA' named entity 'MCF-7 cells' named entity 'infection' named entity 'p53' named entity 'gene expression' named entity 'cell line' named entity 'gene' named entity 'MCF-7' named entity 'endogenous' named entity 'RT-qPCR' named entity 'cellular pathways' named entity 'boxplot' named entity 'primer' named entity 'gene' named entity 'SLC25A4' named entity 'protein' named entity 'reference genes' named entity 'gene expression' named entity 'boxplot' named entity 'sodium pyruvate' named entity 'gene' named entity 'copy number'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software