About: Electrospray ionisation-cleavable tandem nucleic acid mass tag–peptide nucleic acid conjugates: synthesis and applications to quantitative genomic analysis using electrospray ionisation-MS/MS

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Electrospray ionisation-cleavable tandem nucleic acid mass tag–peptide nucleic acid conjugates: synthesis and applications to quantitative genomic analysis using electrospray ionisation-MS/MS Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Electrospray ionisation-cleavable tandem nucleic acid mass tag–peptide nucleic acid conjugates: synthesis and applications to quantitative genomic analysis using electrospray ionisation-MS/MS
Creator	Prescott, Mark Brown, Tom Chelebi, Noorhan Smith, John Thompson, Andrew Nter Schmidt, Gü
Source	PMC
abstract	The synthesis and characterization of isotopomer tandem nucleic acid mass tag–peptide nucleic acid (TNT–PNA) conjugates is described along with their use as electrospray ionisation-cleavable (ESI-Cleavable) hybridization probes for the detection and quantification of target DNA sequences by electrospray ionisation tandem mass spectrometry (ESI-MS/MS). ESI-cleavable peptide TNT isotopomers were introduced into PNA oligonucleotide sequences in a total synthesis approach. These conjugates were evaluated as hybridization probes for the detection and quantification of immobilized synthetic target DNAs using ESI-MS/MS. In these experiments, the PNA portion of the conjugate acts as a hybridization probe, whereas the peptide TNT is released in a collision-based process during the ionization of the probe conjugate in the electrospray ion source. The cleaved TNT acts as a uniquely resolvable marker to identify and quantify a unique target DNA sequence. The method should be applicable to a wide variety of assays requiring highly multiplexed, quantitative DNA/RNA analysis, including gene expression monitoring, genetic profiling and the detection of pathogens.
has issue date	2007-01-26(xsd:dateTime)
bibo:doi	10.1093/nar/gkl1123
bibo:pmid	17259215
has license	cc-by-nc
sha1sum (hex)	6ff8826ca04c185f3a88ab4b0d5a912aea4ab1a6
schema:url	https://doi.org/10.1093/nar/gkl1123
resource representing a document's title	Electrospray ionisation-cleavable tandem nucleic acid mass tag–peptide nucleic acid conjugates: synthesis and applications to quantitative genomic analysis using electrospray ionisation-MS/MS
has PubMed Central identifier	PMC1994780
has PubMed identifier	17259215
schema:publication	Nucleic Acids Res
resource representing a document's body	covid:6ff8826ca04c185f3a88ab4b0d5a912aea4ab1a6#body_text
is schema:about of	named entity 'NUCLEIC ACID' named entity 'PNA' named entity 'electrospray' named entity 'detection' named entity 'evaluated' named entity 'acts' named entity 'mass' named entity 'quantitative' named entity 'SYNTHESIS' named entity 'CONJUGATES' named entity 'PEPTIDE NUCLEIC ACID' named entity 'ANALYSIS' named entity 'INTRODUCED INTO' named entity 'SEQUENCES' named entity 'TARGET' named entity 'METHOD' named entity 'CONJUGATES' named entity 'ESI' named entity 'PEPTIDE NUCLEIC ACID' named entity 'VARIETY' named entity 'TANDEM' named entity 'APPLICABLE' named entity 'ELECTROSPRAY IONISATION TANDEM MASS SPECTROMETRY' named entity 'NUCLEIC ACID' named entity 'OLIGONUCLEOTIDE' named entity 'PORTION' named entity 'INCLUDING' named entity 'USING' named entity 'TANDEM' named entity 'GENOMIC' named entity 'APPLICATIONS' named entity 'ELECTROSPRAY IONISATION' named entity 'QUANTITATIVE' named entity 'QUANTIFICATION' named entity 'MASS' named entity 'HIGHLY' named entity 'DNA' named entity 'IONIZATION' named entity 'DNA SEQUENCE' named entity 'ION SOURCE' named entity 'THESE' named entity 'COLLISION' named entity 'DESCRIBED' named entity 'UNIQUE' named entity 'WIDE' named entity 'MASS' named entity 'PROBE' named entity 'MONITORING' named entity 'GENE EXPRESSION' named entity 'DNAS' named entity 'GENETIC' named entity 'EVALUATED' named entity 'ANALYSIS' named entity 'DNA SEQUENCES' named entity 'USING' named entity 'PROCESS' named entity 'SYNTHESIS' named entity 'TO IDENTIFY' named entity 'PROBES' named entity 'DETECTION' named entity 'CHARACTERIZATION' named entity 'ESI-MS' named entity 'MARKER' named entity 'CONJUGATE' named entity 'RELEASED' named entity 'EXPERIMENTS' named entity 'SYNTHETIC' named entity 'THEIR' named entity 'ACTS' named entity 'APPROACH' named entity 'PEPTIDE' named entity 'CLEAVED' named entity 'HYBRIDIZATION' named entity 'TOTAL SYNTHESIS' named entity 'TNT' named entity 'BASED'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software