About: Stable Internal Reference Genes for Normalizing Real-Time Quantitative PCR in Baphicacanthus cusia under Hormonal Stimuli and UV Irradiation, and in Different Plant Organs

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About: Stable Internal Reference Genes for Normalizing Real-Time Quantitative PCR in Baphicacanthus cusia under Hormonal Stimuli and UV Irradiation, and in Different Plant Organs Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Stable Internal Reference Genes for Normalizing Real-Time Quantitative PCR in Baphicacanthus cusia under Hormonal Stimuli and UV Irradiation, and in Different Plant Organs
Creator	Chen, Junfeng Chen, Yue Diao, Yong Guo, Zhiying Huang, Yuxiang Tan, Hexin Wang, Guoquan Yu, Jian Zhang, Lei Zhang, Qinglei Huang, Y Deal, Roger Reddy, Palakolanu Xiong, Ai-Sheng
Source	Medline; PMC
abstract	Baphicacanthus cusia (Nees) Bremek, the plant source for many kinds of drugs in traditional Chinese medicine, is widely distributed in South China, especially in Fujian. Recent studies about B. cusia mainly focus on its chemical composition and pharmacological effects, but further analysis of the plant's gene functions and expression is required to better understand the synthesis of its effective compounds. Real-time quantitative polymerase chain reaction (RT-qPCR) is a powerful method for gene expression analysis. It is necessary to select a suitable reference gene for expression normalization to ensure the accuracy of RT-qPCR results. Ten candidate reference genes were selected from the transcriptome datasets of B. cusia in this study, and the expression stability was assessed across 60 samples representing different tissues and organs under various conditions, including ultraviolet (UV) irradiation, hormonal stimuli (jasmonic acid methyl ester and abscisic acid), and in different plant organs. By employing different algorithms, such as geNorm, NormFinder, and BestKeeper, which are complementary approaches based on different statistical procedures, 18S rRNA was found to be the most stable gene under UV irradiation and hormonal stimuli, whereas ubiquitin-conjugating enzyme E2 was the best suitable gene for different plant organs. This novel study aimed to screen for suitable reference genes and corresponding primer pairs specifically designed for gene expression studies in B. cusia, in particular for RT-qPCR analyses.
has issue date	2017-05-03(xsd:dateTime)
bibo:doi	10.3389/fpls.2017.00668
bibo:pmid	28515733
has license	cc-by
sha1sum (hex)	7455510a39b00af17f318760b7da8b37cc336476
schema:url	https://doi.org/10.3389/fpls.2017.00668
resource representing a document's title	Stable Internal Reference Genes for Normalizing Real-Time Quantitative PCR in Baphicacanthus cusia under Hormonal Stimuli and UV Irradiation, and in Different Plant Organs
has PubMed Central identifier	PMC5413499
has PubMed identifier	28515733
schema:publication	Front Plant Sci
resource representing a document's body	covid:7455510a39b00af17f318760b7da8b37cc336476#body_text
is schema:about of	named entity 'datasets' named entity 'acid' named entity 'stimuli' named entity 'study' named entity 'method' named entity 'ultraviolet' named entity 'Quantitative PCR' covid:arg/7455510a39b00af17f318760b7da8b37cc336476 named entity 'Fujian' named entity 'kinds' named entity 'reference' named entity 'reference' named entity 'irradiation' named entity 'organs' named entity 'Real-time' named entity 'plant' named entity 'studies' named entity 'expression' named entity 'normalization' named entity 'primer pairs' named entity 'Plant' named entity 'traditional Chinese medicine' named entity 'plant organs' named entity 'gene' named entity 'hormonal' named entity 'abscisic acid' named entity 'Nees' named entity 'RT-qPCR' named entity 'RT-qPCR' named entity '18S rRNA' named entity 'Irradiation' named entity 'Reference Genes' named entity 'Irradiation' named entity 'Quantitative PCR' named entity 'UV-B' named entity 'expression levels' named entity 'cDNA' named entity 'leukemia' named entity 'tobacco' named entity 'hormonal' named entity 'molecular level' named entity 'RNA' named entity 'plant growth' named entity '2.2' named entity 'Melting curve analysis' named entity 'parsley' named entity 'reference genes' named entity 'gene' named entity 'reference genes' named entity 'fluorescence' named entity 'physiological' named entity 'candidate gene' named entity 'vary considerably' named entity 'organism' named entity 'gene expression' named entity 'traditional Chinese medicine' named entity 'gene' named entity 'UV irradiation' named entity 'Frontiers in Plant Science' named entity 'reference genes' named entity 'MeJA' named entity 'Terpenoid' named entity 'housekeeping genes' named entity 'GAPDH' named entity 'housekeeping genes' named entity 'shikimate' named entity 'gene' named entity 'electrophoresis'

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