About: Membrane Active Peptides Remove Surface Adsorbed Protein Corona From Extracellular Vesicles of Red Blood Cells

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Membrane Active Peptides Remove Surface Adsorbed Protein Corona From Extracellular Vesicles of Red Blood Cells Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Membrane Active Peptides Remove Surface Adsorbed Protein Corona From Extracellular Vesicles of Red Blood Cells
Creator	Singh, Priyanka Beke-Somfai, Tamás Bulyáki, Éva Bősze, Szilvia Juhász, Tünde Kardos, József Kitka, Diána Mihály, Judith Ricci, Maria Varga, Zoltán Zsila, Ferenc Cs, Imola Ic, Szigyártó Kardos, Bulyáki Separovic, Frances Somfai, Beke Szigyártó,
Source	PMC
abstract	Besides the outstanding potential in biomedical applications, extracellular vesicles (EVs) are also promising candidates to expand our knowledge on interactions between vesicular surface proteins and small-molecules which exert biomembrane-related functions. Here we provide mechanistic details on interactions between membrane active peptides with antimicrobial effect (MAPs) and red blood cell derived EVs (REVs) and we demonstrate that they have the capacity to remove members of the protein corona from REVs even at lower than 5 μM concentrations. In case of REVs, the Soret-band arising from the membrane associated hemoglobins allowed to follow the detachment process by flow-Linear Dichroism (flow-LD). Further on, the significant change on the vesicle surfaces was confirmed by transmission electron microscopy (TEM). Since membrane active peptides, such as melittin have the affinity to disrupt vesicles, a combination of techniques, fluorescent antibody labeling, microfluidic resistive pulse sensing, and flow-LD were employed to distinguish between membrane destruction and surface protein detachment. The removal of protein corona members is a newly identified role for the investigated peptides, which indicates complexity of their in vivo function, but may also be exploited in synthetic and natural nanoparticle engineering. Furthermore, results also promote that EVs can be used as improved model systems for biophysical studies providing insight to areas with so far limited knowledge.
has issue date	2020-08-11(xsd:dateTime)
bibo:doi	10.3389/fchem.2020.00703
has license	cc-by
sha1sum (hex)	7ddc716f5a9095b68ee5e1e7383f57c5474bcfad
schema:url	https://doi.org/10.3389/fchem.2020.00703
resource representing a document's title	Membrane Active Peptides Remove Surface Adsorbed Protein Corona From Extracellular Vesicles of Red Blood Cells
has PubMed Central identifier	PMC7432246
schema:publication	Front Chem
resource representing a document's body	covid:7ddc716f5a9095b68ee5e1e7383f57c5474bcfad#body_text
is schema:about of	named entity 'hemoglobins' named entity 'improved' named entity 'Red' named entity 'REMOVAL' named entity 'STUDIES' named entity 'SENSING' named entity 'INTERACTIONS' named entity 'MEMBERS' named entity 'FOLLOW' named entity 'IDENTIFIED' named entity 'VESICLES' named entity 'MOLECULES' named entity 'COMPLEXITY' named entity 'INSIGHT' named entity 'COMBINATION' named entity 'BIOPHYSICAL' named entity 'LOWER' named entity 'BAND' named entity 'SURFACES' named entity 'Since' named entity 'interactions' named entity 'red blood cell' named entity 'corona' named entity 'EVs' named entity 'studies' named entity 'employed' named entity 'combination' named entity 'protein' named entity 'synthetic' named entity 'mechanistic' named entity 'vesicle' named entity 'resistive pulse sensing' named entity 'biomembrane' named entity 'antimicrobial' named entity 'red blood cell' named entity 'protein' named entity 'Adsorbed' named entity 'MRPS' named entity 'Absorbance' named entity 'adsorbed' named entity 'human blood' named entity 'structural properties' named entity 'peptides' named entity 'TEM' named entity 'size-exclusion chromatography' named entity 'SEC' named entity 'melittin' named entity 'differential centrifugation' named entity 'antibody' named entity 'pyrene' named entity 'phycoerythrin' named entity 'vesicle' named entity 'spectrometer' named entity 'melittin' named entity 'cecropin' named entity 'chromatogram' named entity 'melittin' named entity 'centrifuge' named entity 'β-sheet' named entity 'peptides' named entity 'melittin' named entity 'peptide' named entity 'Flu' named entity 'membrane models' named entity 'glycophorin' named entity 'α-helical' named entity 'SEC'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software