About: Enterokinase Enhances Influenza A Virus Infection by Activating Trypsinogen in Human Cell Lines

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About: Enterokinase Enhances Influenza A Virus Infection by Activating Trypsinogen in Human Cell Lines Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Enterokinase Enhances Influenza A Virus Infection by Activating Trypsinogen in Human Cell Lines
Creator	Nishimura, Hidekazu Yamaya, Mutsuo Hayashi, Hideki Kubo, Yoshinao Matsuyama, Toshifumi Izumida, Mai Kido, Hiroshi Sato, Ko Campos, Samuel Kuriakose, Teneema Nakayama, Kou Takahashi, Etsuhisa
source	PMC
abstract	Cleavage and activation of hemagglutinin (HA) by trypsin-like proteases in influenza A virus (IAV) are essential prerequisites for its successful infection and spread. In host cells, some transmembrane serine proteases such as TMPRSS2, TMPRSS4 and HAT, along with plasmin in the bloodstream, have been reported to cleave the HA precursor (HA(0)) molecule into its active forms, HA(1) and HA(2). Some trypsinogens can also enhance IAV proliferation in some cell types (e.g., rat cardiomyoblasts). However, the precise activation mechanism for this process is unclear, because the expression level of the physiological activator of the trypsinogens, the TMPRSS15 enterokinase, is expected to be very low in such cells, with the exception of duodenal cells. Here, we show that at least two variant enterokinases are expressed in various human cell lines, including A549 lung-derived cells. The exogenous expression of these enterokinases was able to enhance the proliferation of IAV in 293T human kidney cells, but the proliferation was reduced by knocking down the endogenous enterokinase in A549 cells. The enterokinase was able to enhance HA processing in the cells, which activated trypsinogen in vitro and in the IAV-infected cells also. Therefore, we conclude that enterokinase plays a role in IAV infection and proliferation by activating trypsinogen to process viral HA in human cell lines.
has issue date	2018-03-23(xsd:dateTime)
bibo:doi	10.3389/fcimb.2018.00091
bibo:pmid	29629340
has license	cc-by
sha1sum (hex)	7edf3278c1d0e8577856f24bb6c3eac949201e60
schema:url	https://doi.org/10.3389/fcimb.2018.00091
resource representing a document's title	Enterokinase Enhances Influenza A Virus Infection by Activating Trypsinogen in Human Cell Lines
has PubMed Central identifier	PMC5876233
has PubMed identifier	29629340
schema:publication	Front Cell Infect Microbiol
resource representing a document's body	covid:7edf3278c1d0e8577856f24bb6c3eac949201e60#body_text
is schema:about of	named entity 'expression' named entity 'activated' named entity 'expressed' named entity 'IAV' named entity 'enterokinase' named entity 'Cell' named entity 'Lines' named entity 'ACTIVATION MECHANISM' named entity 'EXPRESSION' named entity 'RAT' named entity 'TMPRSS15' named entity 'INFLUENZA A VIRUS' named entity 'CONCLUDE' named entity '293T' named entity 'HAT' named entity 'CELLS' named entity 'ROLE' named entity 'PLASMIN' named entity 'FORMS' named entity 'ESSENTIAL' named entity 'proliferation' named entity 'forms' named entity 'IAV' named entity 'cleave' named entity 'enhance' named entity 'successful' named entity 'bloodstream' named entity 'proteases' named entity 'serine' named entity 'activation' named entity 'process' named entity 'endogenous' named entity 'IAV' named entity 'IAV' named entity 'physiological' named entity 'infection' named entity 'IAV' named entity 'A549' named entity '293T' named entity 'transmembrane' named entity 'molecule' named entity 'influenza A virus' named entity 'proteases' named entity 'IAV' named entity 'duodenal' named entity 'Influenza A Virus' named entity 'Infection' named entity 'Human Cell Lines' named entity 'hemagglutinin' named entity 'Trypsinogen' named entity 'infection' named entity 'Enterokinase' named entity 'IAV' named entity 'human cell lines' named entity 'trypsinogen' named entity 'bloodstream' named entity 'exogenous' named entity 'TMPRSS4' named entity 'lines' named entity 'cells' named entity 'expression' named entity 'serine proteases' named entity 'enterokinase' named entity 'TMPRSS2' named entity 'KIDNEY' named entity 'IN VITRO' named entity 'A549' named entity 'kidney cells' named entity 'cell types' named entity 'TMPRSS2'

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