About: Prospective comparison of R-mix™ shell vial system with direct antigen tests and conventional cell culture for respiratory virus detection

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Prospective comparison of R-mix™ shell vial system with direct antigen tests and conventional cell culture for respiratory virus detection Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Prospective comparison of R-mix™ shell vial system with direct antigen tests and conventional cell culture for respiratory virus detection
Creator	Ismail, Nahed Smith, Michael Bufton, Kimberly Lasala, P
Source	Elsevier; Medline; PMC
abstract	BACKGROUND AND OBJECTIVES: Conventional cell culture (CC) has limited clinical utility as a result of the extended incubation period often required for virus isolation. Alternative methodologies have been introduced in an effort to improve turnaround times. One such system, the R-mix™ shell vial is discussed herein. The study objectives were: (a) to establish R-mix™ testing parameters as compared to direct antigen testing (DAT) and CC, and (b) to assess technical aspects and cost of R-mix™ in a high volume clinical virology laboratory. STUDY DESIGN: A prospective analysis of respiratory samples submitted to the clinical virology laboratory between November 2004 and April 2005 was performed. All specimens were inoculated onto R-mix™ shell vials (SV) and CC tubes; and a subset also underwent DAT for influenza A and B and/or RSV. A retrospective estimated cost analysis was made. RESULTS: A total of 563 samples were included in the study, which collectively revealed a total of 207 viruses. Sensitivity of R-mix™ for seven major respiratory viruses ranged from 45% to 83% compared to CC and DAT, while mean time to detection (TTD) varied from 1.1 to 1.4 days. In addition to these viruses, 23 picornaviruses, 11 CMV isolates and 5 HSV isolates were detected by CC alone. CONCLUSIONS: The R-mix™ system has similar sensitivity as CC for the detection of parainfluenza 1–3 and influenza A/B while dramatically reducing the TTD. Furthermore, it is significantly more sensitive and produces more timely results for RSV than CC; yet, neither method offers a diagnostic benefit over rapid DAT for RSV detection. The sensitivity of R-mix™ for adenovirus appears to be significantly lower than that of CC. Lastly, methodologies other than R-mix™ must remain in place under circumstances where identification of other potential viral respiratory pathogens, including herpesviruses and picornaviruses, is desired.
has issue date	2007-01-16(xsd:dateTime)
bibo:doi	10.1016/j.jcv.2006.12.015
bibo:pmid	17229589
has license	no-cc
sha1sum (hex)	81448d8f78a47afb41bd9c29a74b73ec8b6dfde9
schema:url	https://doi.org/10.1016/j.jcv.2006.12.015
resource representing a document's title	Prospective comparison of R-mix™ shell vial system with direct antigen tests and conventional cell culture for respiratory virus detection
has PubMed Central identifier	PMC7108409
has PubMed identifier	17229589
schema:publication	J Clin Virol
resource representing a document's body	covid:81448d8f78a47afb41bd9c29a74b73ec8b6dfde9#body_text
is schema:about of	named entity 'DIRECT' named entity 'study' named entity 'picornaviruses' named entity 'revealed' named entity 'vials' named entity 'ranged' named entity 'cell culture' named entity 'TTD' named entity 'direct' named entity 'tubes' named entity 'result' named entity 'retrospective' named entity 'remain' named entity 'vial' named entity 'tests' named entity 'COMPARISON' named entity 'CONVENTIONAL' named entity 'CELL CULTURE' named entity 'VIAL' named entity 'RETROSPECTIVE' named entity 'METHODOLOGIES' named entity 'TECHNICAL' named entity 'CONCLUSIONS' named entity 'APRIL' named entity 'TTD' named entity 'STUDY DESIGN' named entity 'RESPIRATORY PATHOGENS' named entity 'TOTAL' named entity 'EXTENDED' named entity 'PERFORMED' named entity 'ANALYSIS' named entity 'HSV' named entity 'AS A RESULT OF' named entity 'CONVENTIONAL' named entity 'ALTERNATIVE' named entity 'DETECTED' named entity 'SENSITIVITY' named entity 'IDENTIFICATION' named entity 'HIGH VOLUME' named entity 'COMPARED' named entity 'VIRUS ISOLATION' named entity 'WHERE' named entity 'CLINICAL VIROLOGY' named entity 'ADENOVIRUS' named entity 'PLACE' named entity 'VIRUS DETECTION' named entity 'SYSTEM' covid:arg/81448d8f78a47afb41bd9c29a74b73ec8b6dfde9 named entity 'REDUCING' named entity 'HAVE' named entity 'TUBES' named entity 'CMV' named entity 'RESPIRATORY VIRUSES' named entity 'MIX' named entity 'SENSITIVE' named entity 'REVEALED' named entity 'OBJECTIVES' named entity 'MIX' named entity 'PROSPECTIVE' named entity 'SHELL' named entity 'RESPIRATORY VIRUS' named entity 'ANTIGEN TESTS' named entity 'DAT' named entity 'RSV' named entity 'INTRODUCED' named entity 'THESE' named entity 'ADDITION' named entity 'DIAGNOSTIC' named entity '1.1' named entity '1.4' named entity 'PROSPECTIVE' named entity 'HERPESVIRUSES' named entity 'METHOD' named entity 'LOWER' named entity 'NEITHER' named entity 'RESULTS' named entity 'PICORNAVIRUSES' named entity 'SIMILAR'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software