About: Robotic High-Throughput Biomanufacturing and Functional Differentiation of Human Pluripotent Stem Cells

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Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Robotic High-Throughput Biomanufacturing and Functional Differentiation of Human Pluripotent Stem Cells
Creator	Simeonov, Anton Barnaeva, Elena Bonney, Charles Braisted, John Chu, Pei-Hsuan Dorjsuren, Dorjbal Gedik, Yeliz Iannotti, Michael Jovanovic, Vukasin Mallanna, Sunil Malley, Claire Michael, Sam Ormanoglu, Pinar Singeç, Ilyas Slamecka, Jaroslav Tristan, Carlos Voss, Ty
source	BioRxiv; Medline
abstract	Efficient translation of human induced pluripotent stem cells (hiPSCs) depends on implementing scalable cell manufacturing strategies that ensure optimal self-renewal and functional differentiation. Currently, manual culture of hiPSCs is highly variable and labor-intensive posing significant challenges for high-throughput applications. Here, we established a robotic platform and automated all essential steps of hiPSC culture and differentiation under chemically defined conditions. This streamlined approach allowed rapid and standardized manufacturing of billions of hiPSCs that can be produced in parallel from up to 90 different patient-and disease-specific cell lines. Moreover, we established automated multi-lineage differentiation to generate primary embryonic germ layers and more mature phenotypes such as neurons, cardiomyocytes, and hepatocytes. To validate our approach, we carefully compared robotic and manual cell culture and performed molecular and functional cell characterizations (e.g. bulk culture and single-cell transcriptomics, mass cytometry, metabolism, electrophysiology, Zika virus experiments) in order to benchmark industrial-scale cell culture operations towards building an integrated platform for efficient cell manufacturing for disease modeling, drug screening, and cell therapy. Combining stem cell-based models and non-stop robotic cell culture may become a powerful strategy to increase scientific rigor and productivity, which are particularly important during public health emergencies (e.g. opioid crisis, COVID-19 pandemic).
has issue date	2020-08-03(xsd:dateTime)
bibo:doi	10.1101/2020.08.03.235242
bibo:pmid	32793899
has license	biorxiv
sha1sum (hex)	8542f922ca81d9c09e6b970e232d22835ff0d718
schema:url	https://doi.org/10.1101/2020.08.03.235242
resource representing a document's title	Robotic High-Throughput Biomanufacturing and Functional Differentiation of Human Pluripotent Stem Cells
has PubMed identifier	32793899
schema:publication	bioRxiv
resource representing a document's body	covid:8542f922ca81d9c09e6b970e232d22835ff0d718#body_text
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