About: Severe Acute Respiratory Syndrome Coronavirus Nonstructural Proteins 3, 4, and 6 Induce Double-Membrane Vesicles

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Severe Acute Respiratory Syndrome Coronavirus Nonstructural Proteins 3, 4, and 6 Induce Double-Membrane Vesicles
Creator	Neuman, Benjamin Akhlaghpour, Marzieh Angelini, Megan Buchmeier, Michael
Source	Medline; PMC
abstract	Coronaviruses (CoV), like other positive-stranded RNA viruses, redirect and rearrange host cell membranes for use as part of the viral genome replication and transcription machinery. Specifically, coronaviruses induce the formation of double-membrane vesicles in infected cells. Although these double-membrane vesicles have been well characterized, the mechanism behind their formation remains unclear, including which viral proteins are responsible. Here, we use transfection of plasmid constructs encoding full-length versions of the three transmembrane-containing nonstructural proteins (nsps) of the severe acute respiratory syndrome (SARS) coronavirus to examine the ability of each to induce double-membrane vesicles in tissue culture. nsp3 has membrane disordering and proliferation ability, both in its full-length form and in a C-terminal-truncated form. nsp3 and nsp4 working together have the ability to pair membranes. nsp6 has membrane proliferation ability as well, inducing perinuclear vesicles localized around the microtubule organizing center. Together, nsp3, nsp4, and nsp6 have the ability to induce double-membrane vesicles that are similar to those observed in SARS coronavirus-infected cells. This activity appears to require the full-length form of nsp3 for action, as double-membrane vesicles were not seen in cells coexpressing the C-terminal truncation nsp3 with nsp4 and nsp6.
has issue date	2013-08-13(xsd:dateTime)
bibo:doi	10.1128/mbio.00524-13
bibo:pmid	23943763
has license	cc-by-nc-sa
sha1sum (hex)	8682e080613d625a9f6955e5eb0fe80bbbaefda9
schema:url	https://doi.org/10.1128/mbio.00524-13
resource representing a document's title	Severe Acute Respiratory Syndrome Coronavirus Nonstructural Proteins 3, 4, and 6 Induce Double-Membrane Vesicles
has PubMed Central identifier	PMC3747587
has PubMed identifier	23943763
schema:publication	mBio
resource representing a document's body	covid:8682e080613d625a9f6955e5eb0fe80bbbaefda9#body_text
is schema:about of	named entity 'PLASMID' named entity 'VIRAL PROTEINS' named entity 'UNCLEAR' named entity 'VIRAL GENOME REPLICATION ' named entity 'PROLIFERATION' named entity 'PAIR' named entity 'ENCODING' named entity 'ABILITY' named entity 'TRUNCATION' named entity 'INDUCE' named entity 'ACTIVITY' named entity 'TRANSMEMBRANE' named entity 'NSP4' named entity 'LIKE' named entity 'DOUBLE' named entity 'HAVE' named entity 'TRANSCRIPTION' named entity 'CELL MEMBRANES' named entity 'coronaviruses' named entity 'cells' named entity 'infected' named entity 'responsible' named entity 'Proteins' named entity 'Severe Acute Respiratory Syndrome Coronavirus' named entity 'HOST CELL' named entity 'MACHINERY' named entity 'CORONAVIRUSES' named entity 'VESICLES' named entity 'INDUCE' named entity 'DOUBLE' named entity 'NONSTRUCTURAL PROTEINS' named entity 'MEMBRANE' named entity 'INFECTED' named entity 'LOCALIZED' named entity 'TO EXAMINE' named entity 'PERINUCLEAR' named entity 'CELLS' named entity 'PART OF' named entity 'NSP3' named entity 'VERSIONS' named entity 'SEVERE ACUTE RESPIRATORY SYNDROME CORONAVIRUS' named entity 'NOT SEEN' named entity 'INCLUDING' named entity 'MEMBRANE' named entity 'CONTAINING' named entity 'CORONAVIRUS' named entity 'MICROTUBULE ORGANIZING CENTER' named entity 'TRUNCATED' named entity 'HERE' named entity 'FORMATION' named entity 'SIMILAR' named entity 'TRANSFECTION' named entity 'VESICLES' named entity 'OBSERVED' named entity 'SEVERE ACUTE RESPIRATORY SYNDROME' named entity 'POSITIVE' named entity 'INDUCING' named entity 'THESE' named entity 'RNA VIRUSES' named entity 'THEIR' named entity 'TISSUE CULTURE' named entity 'MECHANISM' named entity 'FORM' named entity 'WORKING' named entity 'NONSTRUCTURAL PROTEINS' named entity 'RESPONSIBLE' named entity 'CHARACTERIZED' named entity 'ITS' named entity 'SARS CORONAVIRUS' named entity 'C-TERMINAL' named entity 'USE' named entity 'MEMBRANES' named entity 'LENGTH' named entity 'ACTION' named entity 'OF EACH' covid:arg/8682e080613d625a9f6955e5eb0fe80bbbaefda9 named entity 'perinuclear' named entity 'cells'

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