About: A Human DPP4-Knockin Mouse’s Susceptibility to Infection by Authentic and Pseudotyped MERS-CoV

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	A Human DPP4-Knockin Mouse’s Susceptibility to Infection by Authentic and Pseudotyped MERS-CoV
Creator	Jiang, Shibo Ying, Tianlei Cao, Yuan Liu, Qiang Huang, Weijin Wang, Youchun Fan, Changfa Li, Qianqian Liu, Susu Wu, Xi Liang, Chunnan Lu, Jianjun Yang, Yanwei
source	Medline; PMC
abstract	Infection by the Middle East respiratory syndrome coronavirus (MERS-CoV) causes respiratory illness and has a high mortality rate (~35%). The requirement for the virus to be manipulated in a biosafety level three (BSL-3) facility has impeded development of urgently-needed antiviral agents. Here, we established anovel mouse model by inserting human dipeptidyl peptidase 4 (hDPP4) into the Rosa26 locus using CRISPR/Cas9, resulting in global expression of the transgene in a genetically stable mouse line. The mice were highly susceptible to infection by MERS-CoV clinical strain hCoV-EMC, which induced severe diffuse pulmonary disease in the animals, and could also be infected by an optimized pseudotyped MERS-CoV. Administration of the neutralizing monoclonal antibodies, H111-1 and m336, as well as a fusion inhibitor peptide, HR2P-M2, protected mice from challenge with authentic and pseudotyped MERS-CoV. These results confirmed that the hDPP4-knockin mouse is a novel model for studies of MERS-CoV pathogenesis and anti-MERS-CoV antiviral agents in BSL-3 and BSL-2facilities, respectively.
has issue date	2018-08-23(xsd:dateTime)
bibo:doi	10.3390/v10090448
bibo:pmid	30142928
has license	cc-by
sha1sum (hex)	874e540a730ee1060365af8d2caa03f537508e33
schema:url	https://doi.org/10.3390/v10090448
resource representing a document's title	A Human DPP4-Knockin Mouse’s Susceptibility to Infection by Authentic and Pseudotyped MERS-CoV
has PubMed Central identifier	PMC6164841
has PubMed identifier	30142928
schema:publication	Viruses
resource representing a document's body	covid:874e540a730ee1060365af8d2caa03f537508e33#body_text
is schema:about of	named entity 'Administration' named entity 'MERS-CoV' covid:arg/874e540a730ee1060365af8d2caa03f537508e33 named entity 'human' named entity 'BSL-3' named entity 'MERS-CoV' named entity 'These' named entity 'Here' named entity 'MERS-CoV' named entity 'mouse' named entity 'Middle East respiratory syndrome' named entity 'respiratory illness' named entity 'The' named entity 'DPP4' named entity 'pathogenesis' named entity 'pseudotyped' named entity 'antiviral agents' named entity 'MERS-CoV' named entity 'MERS-CoV' named entity 'transgene' named entity 'BSL-3' named entity 'CRISPR/Cas9' named entity 'fusion inhibitor' named entity 'dipeptidyl peptidase 4' named entity 'Pseudotyped' named entity 'Infection' named entity 'coronavirus' named entity 'goat' named entity 'zygotes' named entity 'wild-type' named entity 'infection' named entity 'bronchial epithelial cells' named entity 'Biological Pharmacy' named entity 'transfected' named entity 'animal model' named entity 'transfected' named entity 'mouse model' named entity 'necrosis' named entity 'microwave oven' named entity 'Pseudovirus' named entity '1:200' named entity 'genomic DNA' named entity 'infection' named entity 'hyaline' named entity 'MERS-CoV' named entity 'RNA' named entity 'viral titers' named entity 'infection' named entity 'sodium pentobarbital' named entity 'genotyped' named entity 'Invitrogen' named entity 'ganglia' named entity 'backcrossed' named entity 'viral load' named entity 'photon' named entity 'Codon' named entity 'PBS' named entity 'infection' named entity 'pseudovirus' named entity 'pseudotyped' named entity 'high-throughput' named entity 'tissue sections' named entity 'CNS' named entity 'liver' named entity 'primers' named entity 'peptide' named entity 'dose-dependent manner' named entity 'Boston' named entity 'pseudovirus'

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