About: Three-Dimensional Architecture and Biogenesis of Membrane Structures Associated with Hepatitis C Virus Replication

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About: Three-Dimensional Architecture and Biogenesis of Membrane Structures Associated with Hepatitis C Virus Replication Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Three-Dimensional Architecture and Biogenesis of Membrane Structures Associated with Hepatitis C Virus Replication
Creator	Bartenschlager, Ralf Lee, Ji-Young Krijnse-Locker, Jacomine Chlanda, Petr Antony, Claude Chiramel, Abhilash Habermann, Anja Haselman, Uta Hoppe, Simone Kallis, Stephanie Merz, Andreas Santarella-Mellwig, Rachel Walther, Paul Romero-Brey, Iné
Source	Medline; PMC
abstract	All positive strand RNA viruses are known to replicate their genomes in close association with intracellular membranes. In case of the hepatitis C virus (HCV), a member of the family Flaviviridae, infected cells contain accumulations of vesicles forming a membranous web (MW) that is thought to be the site of viral RNA replication. However, little is known about the biogenesis and three-dimensional structure of the MW. In this study we used a combination of immunofluorescence- and electron microscopy (EM)-based methods to analyze the membranous structures induced by HCV in infected cells. We found that the MW is derived primarily from the endoplasmic reticulum (ER) and contains markers of rough ER as well as markers of early and late endosomes, COP vesicles, mitochondria and lipid droplets (LDs). The main constituents of the MW are single and double membrane vesicles (DMVs). The latter predominate and the kinetic of their appearance correlates with kinetics of viral RNA replication. DMVs are induced primarily by NS5A whereas NS4B induces single membrane vesicles arguing that MW formation requires the concerted action of several HCV replicase proteins. Three-dimensional reconstructions identify DMVs as protrusions from the ER membrane into the cytosol, frequently connected to the ER membrane via a neck-like structure. In addition, late in infection multi-membrane vesicles become evident, presumably as a result of a stress-induced reaction. Thus, the morphology of the membranous rearrangements induced in HCV-infected cells resemble those of the unrelated picorna-, corona- and arteriviruses, but are clearly distinct from those of the closely related flaviviruses. These results reveal unexpected similarities between HCV and distantly related positive-strand RNA viruses presumably reflecting similarities in cellular pathways exploited by these viruses to establish their membranous replication factories.
has issue date	2012-12-06(xsd:dateTime)
bibo:doi	10.1371/journal.ppat.1003056
bibo:pmid	23236278
has license	cc-by
sha1sum (hex)	8984416a285ced3a8855c5b4a473d02ab50e73d0
schema:url	https://doi.org/10.1371/journal.ppat.1003056
resource representing a document's title	Three-Dimensional Architecture and Biogenesis of Membrane Structures Associated with Hepatitis C Virus Replication
has PubMed Central identifier	PMC3516559
has PubMed identifier	23236278
schema:publication	PLoS Pathog
resource representing a document's body	covid:8984416a285ced3a8855c5b4a473d02ab50e73d0#body_text
is schema:about of	named entity 'vesicles' named entity 'arguing' named entity 'viral' named entity 'early' named entity 'membranous' named entity 'site' named entity 'structure' named entity 'DMVs' named entity 'DMVs' named entity 'Structures' named entity 'Membrane' named entity 'VIRUS REPLICATION' named entity 'THREE-DIMENSIONAL' named entity 'COP' named entity 'DISTANTLY' named entity 'LEE' named entity 'VIRAL RNA' named entity 'REFLECTING' named entity 'STRUCTURES' named entity 'ENDOSOMES' named entity 'REPLICATE' named entity 'EARLY' named entity 'RELATED' named entity 'CONSTITUENTS' named entity 'NECK' named entity 'CORONA' named entity 'SINGLE' named entity 'HCV' named entity 'CONTAINS' named entity 'UNRELATED' named entity 'MEMBRANE STRUCTURES' named entity 'FORMING' named entity 'STRUCTURE' named entity 'CITATION' named entity 'CELLS' named entity 'USED' named entity 'KINETICS' named entity 'STUDY' named entity 'MEMBRANOUS' named entity 'BIOGENESIS' named entity 'DISTINCT FROM' named entity 'INFECTED' named entity 'BUT' named entity 'POSITIVE' named entity 'FOUND' named entity 'THREE-DIMENSIONAL' named entity 'INDUCED REACTION' named entity 'CYTOSOL' named entity 'RESULTS' named entity 'ADDITION' named entity 'ASSOCIATION' named entity 'RNA VIRUSES' named entity 'KINETIC' named entity 'VIRUSES' named entity 'REPLICASE' named entity 'CELLULAR' named entity 'LIPID DROPLETS' named entity 'FACTORIES' named entity 'WEB' named entity 'FORMATION' named entity 'FLAVIVIRUSES' named entity 'REVEAL' named entity 'REARRANGEMENTS' named entity 'ESTABLISH' named entity 'THESE' named entity 'HEPATITIS C VIRUS' named entity 'MEMBRANE STRUCTURES' named entity 'VIRUS REPLICATION'

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