About: A simplified procedure for the subtractive cDNA cloning of photoassimilate-responding genes: isolation of cDNAs encoding a new class of pathogenesis-related proteins

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About: A simplified procedure for the subtractive cDNA cloning of photoassimilate-responding genes: isolation of cDNAs encoding a new class of pathogenesis-related proteins Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	A simplified procedure for the subtractive cDNA cloning of photoassimilate-responding genes: isolation of cDNAs encoding a new class of pathogenesis-related proteins
Creator	Badur, Ralf Herbers, Karin Mrnke, Gudrun Sonnewald, Uwe
Source	PMC
abstract	Transgenic tobacco plants (ppa-1) constitutively expressing Escherichia coli pyrophosphatase behind the 35S CaMV promoter accumulate high levels of soluble sugars in their leaves [27]. These plants were considered a tool to study adaptation of leaves to photoassimilate accumulation at the molecular level. By differential hybridization of a subtractive library enriched for transcripts present in the transgenic plants 12 different cDNAs were isolated. By sequence analysis four cDNAs could be identified as 1-aminocyclopropane-1-carboxylate-oxidase and as three different pathogenesis-related proteins (PR-1b, PR-Q and SAR 8.2). Two cDNAs were homologous to a calmodulin-like protein from Arabidopsis and a human ribosomal protein L19 while six cDNA clones remained unknown. One of these clones (termed PAR-1 for photoassimilate-responsive) displayed features similar to pathogenesis-related proteins: Hybridizing transcripts, 1.2 and 1.0 kb in length, were strongly inducible by salicylate and accumulated in tobacco plants after infection with potato virus Y (PVY) both in infected and uninfected systemic leaves. PAR-1 transcripts also accumulated in wildtype leaves upon floating on glucose and sucrose whereas sorbitol and polyethylene glycol had no effect. Rescreening of the ppa-1 cDNA library with the PAR-1 cDNA as probe resulted in 25 hybridizing cDNAs which by homology were found to fall into three classes (PAR-1a, b, c). The cDNAs coding for PAR-1a and b were 90.6% homologous on the DNA level while both were less related to the PAR-1c cDNA (70.5% and 75.2% homologous, respectively). One open reading frame was identified in all three PAR-1 cDNA classes. Translation would result in proteins with a theoretical molecular mass of about 20 kDa. The N-terminal amino acid sequences resemble a signal peptide which would direct the proteins to the secretory pathway. Using selective 3′ hybridization probes of the three PAR-1 cDNAs it was possible to discriminate the different transcripts. Both PAR-1a and PAR-1c mRNAs are induced in plants treated with PVY.
has issue date	1995-01-01(xsd:dateTime)
bibo:doi	10.1007/bf00014975
bibo:pmid	8555446
has license	no-cc
sha1sum (hex)	89f4bda6c479ca9b60a25181359cfac1f44fef47
schema:url	https://doi.org/10.1007/bf00014975
resource representing a document's title	A simplified procedure for the subtractive cDNA cloning of photoassimilate-responding genes: isolation of cDNAs encoding a new class of pathogenesis-related proteins
has PubMed Central identifier	PMC7088993
has PubMed identifier	8555446
schema:publication	Plant Mol Biol
resource representing a document's body	covid:89f4bda6c479ca9b60a25181359cfac1f44fef47#body_text
is schema:about of	named entity 'proteins' named entity 'transcripts' named entity 'ribosomal protein' named entity 'One' named entity 'identified' named entity 'PAR' named entity 'sorbitol' named entity 'plants' named entity 'pathogenesis-related' named entity 'potato virus Y' named entity 'identified' named entity 'proteins' named entity 'UNKNOWN' named entity 'SALICYLATE' named entity 'TREATED WITH' named entity 'TRANSGENIC TOBACCO' named entity 'DIFFERENTIAL HYBRIDIZATION' named entity 'CLONES' named entity 'SIMILAR' named entity 'STUDY' named entity 'INFECTED' named entity 'SIGNAL PEPTIDE' named entity 'molecular mass' named entity 'termed' named entity 'result' named entity 'proteins' named entity 'cDNAs' named entity 'leaves' named entity 'probe' named entity 'molecular' named entity 'plants' named entity 'sequences' named entity 'adaptation' named entity 'homologous' named entity 'length' named entity 'PAR' named entity 'cDNA clones' named entity 'amino acid' named entity 'Translation' named entity 'cDNAs' named entity 'sequence analysis' named entity 'CaMV' named entity 'simplified' named entity '1-aminocyclopropane-1-carboxylate' named entity 'Hybridizing' named entity 'signal peptide' named entity 'PAR-1' named entity 'Escherichia coli' named entity 'hybridizing' named entity 'PAR-1' named entity 'Arabidopsis' named entity 'virus' named entity 'N-terminal amino acid' named entity '8.2' named entity 'wildtype' named entity 'glucose' named entity 'photoassimilate' named entity 'cDNAs' named entity 'differential hybridization' named entity 'cDNAs' named entity 'transfected' named entity 'osmotic' named entity 'ribosomal protein' named entity 'cDNAs' named entity 'biochemical' named entity 'plant cells' named entity 'homologous' named entity 'recombinant' named entity 'PAR-1' named entity 'mRNA' named entity 'uronic acids' named entity 'osmotic stress' named entity 'sugar' named entity 'plant material' named entity 'pathogens' named entity 'untranslated regions' named entity 'Stratagene' named entity 'cDNA'

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