About: Characterization of amino acid substitutions in feline coronavirus 3C-like protease from a cat with feline infectious peritonitis treated with a protease inhibitor

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About: Characterization of amino acid substitutions in feline coronavirus 3C-like protease from a cat with feline infectious peritonitis treated with a protease inhibitor Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Characterization of amino acid substitutions in feline coronavirus 3C-like protease from a cat with feline infectious peritonitis treated with a protease inhibitor
Creator	Liu, Hongwei Chang, Kyeong-Ok Kim, Yunjeong Groutas, William Pedersen, Niels Rathnayake, Athri Perera, Krishani
Source	Elsevier; Medline; PMC
abstract	Feline infectious peritonitis (FIP) is a highly fatal disease caused by a virulent feline coronavirus in domestic and wild cats. We have previously reported the synthesis of potent coronavirus 3C-like protease (3CLpro) inhibitors and the efficacy of a protease inhibitor, GC376, in client-owned cats with FIP. In this study, we studied the effect of the amino acid changes in 3CLpro of feline coronavirus from a feline patient who received antiviral treatment for prolonged duration. We generated recombinant 3CLpro containing the identified amino acid changes (N25S, A252S or K260 N) and determined their susceptibility to protease inhibitors in the fluorescence resonance energy transfer assay. The assay showed that N25S in 3CLpro confers a small change (up to 1.68-fold increase in the 50% inhibitory concentration) in susceptibility to GC376, but other amino acid changes do not affect susceptibility. Modelling of 3CLpro carrying the amino acid changes was conducted to probe the structural basis for these findings. The results of this study may explain the observed absence of clinical resistance to the long-term antiviral treatment in the patients.
has issue date	2019-08-23(xsd:dateTime)
bibo:doi	10.1016/j.vetmic.2019.108398
bibo:pmid	31585653
has license	no-cc
sha1sum (hex)	8a88b69a1c54ef2947a0b0c678ca033f0f057462
schema:url	https://doi.org/10.1016/j.vetmic.2019.108398
resource representing a document's title	Characterization of amino acid substitutions in feline coronavirus 3C-like protease from a cat with feline infectious peritonitis treated with a protease inhibitor
has PubMed Central identifier	PMC6779346
has PubMed identifier	31585653
schema:publication	Vet Microbiol
resource representing a document's body	covid:8a88b69a1c54ef2947a0b0c678ca033f0f057462#body_text
is schema:about of	named entity 'resistance' named entity 'feline' named entity 'domestic' named entity 'carrying' named entity 'FELINE INFECTIOUS PERITONITIS' named entity 'FIP' named entity 'CORONAVIRUS' named entity 'EFFECT' named entity 'PROTEASE INHIBITORS' covid:arg/8a88b69a1c54ef2947a0b0c678ca033f0f057462 named entity 'WILD' named entity 'IS A' named entity '1.68' named entity 'ASSAY' named entity 'FATAL DISEASE' named entity 'HAVE' named entity 'RESULTS' named entity 'PROTEASE' named entity 'DO NOT' named entity 'RECOMBINANT' named entity 'RECEIVED' named entity 'CONTAINING' named entity 'AMINO ACID' named entity 'CHARACTERIZATION' named entity 'FELINE CORONAVIRUS' named entity 'AMINO ACID SUBSTITUTIONS' named entity 'FELINE CORONAVIRUS' named entity 'OBSERVED' named entity 'ANTIVIRAL TREATMENT' named entity 'FELINE INFECTIOUS PERITONITIS' named entity 'CAT' named entity 'VIRULENT' named entity 'PATIENTS' named entity 'PROTEASE INHIBITOR' named entity 'LONG-TERM' named entity 'AFFECT' named entity 'CARRYING' named entity 'BUT' named entity 'INHIBITORY' named entity 'FOLD INCREASE' named entity 'CLINICAL RESISTANCE' named entity 'PATIENT' named entity 'THEIR' named entity 'SUSCEPTIBILITY' named entity 'LIKE' named entity 'PROLONGED' named entity 'FINDINGS' named entity 'CATS' named entity 'INHIBITORS' named entity 'THESE' named entity 'GENERATED' named entity 'PROBE' named entity 'CHANGES' named entity 'STRUCTURAL' named entity 'EFFICACY' named entity 'REPORTED' named entity 'ABSENCE OF' named entity 'FLUORESCENCE RESONANCE ENERGY TRANSFER ASSAY' named entity 'SMALL' named entity 'HIGHLY' named entity 'CONCENTRATION' named entity 'RESISTANCE TO' named entity 'DOMESTIC' named entity 'BASIS' named entity 'MODELLING' named entity '50%' named entity 'PREVIOUSLY' named entity 'FELINE' named entity 'DETERMINED' named entity 'PROTEASE INHIBITOR' named entity 'PROTEASE' named entity 'IDENTIFIED' named entity 'STUDY' named entity 'SYNTHESIS' named entity 'TREATED WITH'

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