About: Identification of M.tuberculosis-Specific Th1 Cells Expressing CD69 Generated in vivo in Pleural Fluid Cells from Patients with Tuberculous Pleurisy

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About: Identification of M.tuberculosis-Specific Th1 Cells Expressing CD69 Generated in vivo in Pleural Fluid Cells from Patients with Tuberculous Pleurisy Goto Sponge NotDistinct Permalink

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Identification of M.tuberculosis-Specific Th1 Cells Expressing CD69 Generated in vivo in Pleural Fluid Cells from Patients with Tuberculous Pleurisy
Creator	Wu, Changyou Li, Li Fu, Xiaoying Lao, Suihua Qiao, Dan Zhang, Xianlan
Source	Medline; PMC
abstract	Th1 cell-mediated immune responses at the site of active infection are important to restrict the growth of M.tuberculosis (MTB) and for the spontaneous resolution of patients with tuberculous pleurisy (TBP). In the present study, we found that without any stimulation, CD4(+) T cells in pleural fluid cells (PFCs) from patients with TBP expressed significantly higher levels of CD69 than PBMCs from patients with tuberculosis (TB) or healthy donors. CD4(+)CD69(+) T cells expressed T-bet and IL-12Rβ2. After stimulation with MTB-specific antigens, CD4(+)CD69(+) T cells expressed significantly higher levels of IFN-γ, IL-2 and TNF-α than CD4(+)CD69(−) T cells, demonstrating that CD4(+)CD69(+) T cells were MTB-specific Th1 cells. In addition, CD4(+)CD69(+) T cells were mostly polyfunctional Th1 cells that simultaneously produced IFN-γ, IL-2, TNF-α and displayed an effector or effector memory phenotype (CD45RA(−)CCR7(−)CD62L(−)CD27(−)). Moreover, the percentages of CD4(+)CD69(+) T cells were significantly and positively correlated with polyfunctional T cells. Interestingly, sorted CD4(+)CD69(+) but not CD4(+)CD69(−) fractions by flow cytometry produced IFN-γ, IL-2 and TNF-α that were significantly regulated by CD4(+)CD25(+) Treg cells. Taken together, based on the expression of CD69, we found a direct quantitative and qualitative method to detect and evaluate the in vivo generated MTB-specific polyfunctional CD4(+) T cells in PFCs from patients with TBP. This method can be used for the potential diagnosis and enrichment or isolation of MTB-specific Th1 cells in the investigations.
has issue date	2011-08-22(xsd:dateTime)
bibo:doi	10.1371/journal.pone.0023700
bibo:pmid	21887301
has license	cc-by
sha1sum (hex)	8f26b1ab1d54522ce151927a8cb48cf52058961a
schema:url	https://doi.org/10.1371/journal.pone.0023700
resource representing a document's title	Identification of M.tuberculosis-Specific Th1 Cells Expressing CD69 Generated in vivo in Pleural Fluid Cells from Patients with Tuberculous Pleurisy
has PubMed Central identifier	PMC3161751
has PubMed identifier	21887301
schema:publication	PLoS One
resource representing a document's body	covid:8f26b1ab1d54522ce151927a8cb48cf52058961a#body_text
is schema:about of	named entity 'EVALUATE' named entity 'CD4' named entity 'ISOLATION' named entity 'GROWTH' named entity 'STUDY' named entity 'IFN' named entity 'GENERATED' named entity 'IMPORTANT' named entity 'CD69' named entity 'TH1 CELLS' named entity 'PLEURAL FLUID' named entity 'LAO' named entity 'IDENTIFICATION' named entity 'ACTIVE' named entity 'ADDITION' named entity 'METHOD' named entity 'BASED' named entity 'PBMCS' named entity 'DISPLAYED' named entity 'TAKEN' named entity 'QUALITATIVE' named entity 'CITATION' named entity 'GENERATED' named entity 'IDENTIFICATION' named entity 'SPECIFIC' named entity 'SPONTANEOUS RESOLUTION' named entity 'TREG' named entity 'CD69' named entity 'INVESTIGATIONS' named entity 'SPECIFIC' named entity 'PATIENTS' named entity 'CELLS' named entity 'TUBERCULOSIS' named entity 'IN VIVO' named entity 'DIRECT' named entity '288' named entity 'RESTRICT' named entity 'MEDIATED' named entity 'QUANTITATIVE' named entity 'T CELLS' named entity 'SITE OF' named entity 'ENRICHMENT' named entity 'TUBERCULOUS PLEURISY' named entity 'CD25' named entity 'T-BET' named entity 'BUT' named entity 'MEMORY PHENOTYPE' named entity 'MTB' named entity 'USED' named entity 'TO DETECT' named entity 'IL-12RB2' named entity 'CD27' named entity 'EXPRESSION' named entity 'ZHANG' named entity 'PFCS' named entity 'REGULATED' named entity 'PRESENT' named entity 'PATIENTS' named entity 'TH1 CELL' named entity 'CD62L' named entity 'EXPRESSED' named entity 'SIMULTANEOUSLY' named entity 'SPECIFIC ANTIGENS' named entity 'POSITIVELY CORRELATED' named entity 'TUBERCULOSIS' named entity 'DIAGNOSIS' named entity 'POTENTIAL' named entity 'TH1 CELLS' named entity 'TNF-A' named entity 'CCR7' named entity 'EFFECTOR' named entity 'STIMULATION' named entity 'LEVELS' named entity 'TUBERCULOUS PLEURISY' named entity 'PLEURAL FLUID' named entity 'DONORS'

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