About: High-sensitivity COVID-19 group testing by digital PCR

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: High-sensitivity COVID-19 group testing by digital PCR Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	High-sensitivity COVID-19 group testing by digital PCR
Creator	Visseaux, Benoit André, Barbara Dangla, Rémi Gossner, Olivier Mallory, Allison Martin, Alexandra Storto, Alexandre
Source	ArXiv
abstract	Background: Worldwide demand for SARS-CoV-2 RT-PCR testing is increasing as more countries are impacted by COVID-19 and as testing remains central to contain the spread of the disease, both in countries where the disease is emerging and in countries that are past the first wave but exposed to re-emergence. Group testing has been proposed as a solution to expand testing capabilities but sensitivity concerns have limited its impact on the management of the pandemic. Digital PCR (RT-dPCR) has been shown to be more sensitive than RT-PCR and could help in this context. Methods: We implemented RT-dPCR based COVID-19 group testing on commercially available system and assay (Naica System from Stilla Technologies) and investigated the sensitivity of the method in real life conditions of a university hospital in Paris, France, in May 2020. We tested the protocol in a direct comparison with reference RT-PCR testing on 448 samples split into groups of 3 sizes for RT-dPCR analysis: 56 groups of 8 samples, 28 groups of 16 samples and 14 groups of 32 samples. Results: Individual RT-PCR testing identified 25 positive samples. Using groups of 8, testing by RT-dPCR identified 23 groups as positive, corresponding to 26 true positive samples including 2 samples not initially detected by individual RT-PCR but confirmed positive by further RT-PCR and RT-dPCR investigation. For groups of 16, 15 groups tested positive, corresponding to 25 true positive samples identified. 100% concordance is found for groups of 32 but with limited data points.
has issue date	2020-06-03(xsd:dateTime)
has license	arxiv
sha1sum (hex)	90ea0d87e6c90695278dd75b2fcd78dda9eabf28
resource representing a document's title	High-sensitivity COVID-19 group testing by digital PCR
resource representing a document's body	covid:90ea0d87e6c90695278dd75b2fcd78dda9eabf28#body_text
is schema:about of	named entity 'testing' named entity 'Technologies' named entity 'groups' named entity 'May' named entity 'Group testing' named entity 'Methods' named entity 'testing' named entity 'impacted' named entity 'samples' named entity 'group testing' named entity 'ASSAY' named entity 'CONDITIONS' named entity 'WHERE' named entity 'UNIVERSITY HOSPITAL' named entity 'INCREASING' named entity 'COVID-19' named entity 'IMPACTED' named entity 'SARS-COV-2' named entity 'PROTOCOL' named entity 'BACKGROUND' named entity 'COUNTRIES' named entity 'CONTEXT' covid:arg/90ea0d87e6c90695278dd75b2fcd78dda9eabf28 named entity 'management' named entity 'sensitivity' named entity 'France' named entity 'Digital PCR' named entity 'COVID' named entity 'group testing' named entity 'Group testing' named entity 'disease' named entity 'Group ID' named entity 'PCR' named entity 'viruses' named entity 'France' named entity 'SARS-CoV-2' named entity 'Cobas' named entity 'RT-PCR' named entity 'SARS-CoV2' named entity 'RT-PCR' named entity 'titration' named entity 'group testing' named entity 'Switzerland' named entity 'true positive' named entity 'RT-PCR' named entity 'titration' named entity 'group testing' named entity 'P16' named entity 'transport medium' named entity '10.5' named entity 'RT-PCR' named entity 'SARS-CoV-2' named entity 'Naica' named entity 'Paris' named entity 'PCR' named entity 'SARS-CoV-2' named entity 'RT-PCR' named entity 'RT-PCR' named entity 'RT-PCR' named entity 'PCR' named entity 'RT-PCR' named entity 'Nebraska' named entity 'Altona' named entity 'Digital PCR' named entity 'RT-PCR' named entity 'group testing' named entity 'ORF' named entity 'RT-PCR' named entity 'gold standard' named entity 'confidence level' named entity 'PCR' named entity 'PCR' named entity 'RT-PCR' named entity 'SARS-CoV-2' named entity 'RT-PCR' named entity 'reagent' named entity 'SARS-CoV-2' named entity 'RT-PCR' named entity 'digital PCR' named entity 'Naica' named entity 'digital PCR'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software