About: A fast and simple one-step duplex PCR assay for canine distemper virus (CDV) and canine coronavirus (CCoV) detection

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About: A fast and simple one-step duplex PCR assay for canine distemper virus (CDV) and canine coronavirus (CCoV) detection Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	A fast and simple one-step duplex PCR assay for canine distemper virus (CDV) and canine coronavirus (CCoV) detection
Creator	Wang, Jing Cui, Shangjin Li, Jinxiang Liang, Lin Luo, Yakun
source	Medline; PMC
abstract	The one-step polymerase chain reaction (one-step PCR) detection assay is an innovative PCR detection method, eliminating nucleic acid extraction steps, in which samples can be directly added to PCR reagents for testing. For simultaneous detection of CDV and CCoV, a sensitive and specific one-step duplex PCR (one-step dPCR) assay was developed with two pairs of primers that were designed based on H and M gene sequences of CDV and CCoV, respectively. The one-step dPCR with optimized detection conditions has high specificity and sensitivity; independent sequencing assays further verified these results.
has issue date	2018-08-23(xsd:dateTime)
bibo:doi	10.1007/s00705-018-3982-8
bibo:pmid	30141131
has license	no-cc
sha1sum (hex)	918ee8954f1c27dfdc36320da11a57b9e53a4322
schema:url	https://doi.org/10.1007/s00705-018-3982-8
resource representing a document's title	A fast and simple one-step duplex PCR assay for canine distemper virus (CDV) and canine coronavirus (CCoV) detection
has PubMed Central identifier	PMC7087121
has PubMed identifier	30141131
schema:publication	Arch Virol
resource representing a document's body	covid:918ee8954f1c27dfdc36320da11a57b9e53a4322#body_text
is schema:about of	named entity 'reagents' named entity 'simultaneous' named entity 'one-step' named entity 'CANINE CORONAVIRUS' named entity 'DETECTION' named entity 'SAMPLES' named entity 'ASSAY' named entity 'DETECTION' named entity 'M GENE' named entity 'VERIFIED' named entity 'PAIRS' named entity 'SENSITIVE' named entity 'SEQUENCING ASSAYS' named entity 'PRIMERS' named entity 'NUCLEIC ACID EXTRACTION' named entity 'SIMULTANEOUS' named entity 'CONDITIONS' named entity 'THESE' named entity 'CANINE DISTEMPER VIRUS' named entity 'FAST' named entity 'PCR ASSAY' named entity 'STEP' named entity 'SIMPLE' named entity 'HIGH' named entity 'CDV' named entity 'POLYMERASE CHAIN REACTION' named entity 'TESTING' named entity 'SPECIFIC' named entity 'DIRECTLY' named entity 'STEPS' named entity 'RESULTS' named entity 'INDEPENDENT' named entity 'ADDED' named entity 'SEQUENCES' named entity 'BASED' named entity 'REAGENTS' named entity 'DPCR' named entity 'SPECIFICITY' named entity 'STEP' named entity 'DETECTION METHOD' named entity 'PCR' named entity 'SENSITIVITY' covid:arg/918ee8954f1c27dfdc36320da11a57b9e53a4322 named entity 'specificity' named entity 'detection' named entity 'verified' named entity 'sequences' named entity 'sequencing' named entity 'optimized' named entity 'fast' named entity 'CDV' named entity 'gene sequences' named entity 'assay' named entity 'sensitive and specific' named entity 'primers' named entity 'nucleic acid' named entity 'canine distemper virus' named entity 'assay' named entity 'cDNA' named entity 'conserved regions' named entity 'coinfection' named entity 'CDV' named entity 'primers' named entity 'Shanghai' named entity 'primers' named entity 'early diagnosis' named entity 'China' named entity 'CDV' named entity 'virus' named entity 'recombinant' named entity 'serial dilution' named entity 'gene' named entity 'nonstructural proteins' named entity 'RNA' named entity 'DNA sequencing' named entity 'protein' named entity 'PCR'

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