About: Development and application of a novel ELISA for detecting antibodies against group I fowl adenoviruses

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Development and application of a novel ELISA for detecting antibodies against group I fowl adenoviruses
Creator	Zhang, Yanping Li, Kai Wang, Jing Gao, Li Cui, Hongyu Wang, Yongqiang Gao, Yulong Liu, Changjun Qi, Xiaole Pan, Qing Wang, &
Source	PMC
abstract	Since 2015, outbreaks of hepatitis-hydropericardium syndrome (HPS) caused by a novel genotype of fowl adenovirus 4 (FAdV-4) infection have created serious economic losses in China. Given that other serotypes of hypervirulent FAdVs have also been reported in poultry around the world, a common ELISA for all serotypes within the group I fowl adenoviruses (FAdV-I) is urgently needed, especially for clinical epidemic serotypes. In this study, we used high purity and concentration virions of FAdV-4 and developed a common ELISA for detecting antibodies against 12 FAdV-I serotypes. The developed ELISA was able to distinguish between antibodies against FAdV-I, FAdV-III, and other heterologous viruses without any cross-reaction. Furthermore, the ELISA showed higher sensitivity than the FAdV-1-based ELISA to the novel FAdV-4 found in China. Moreover, since there are no commercial vaccines against FAdVs in China, the ELISA was applied to detect sera samples from specific pathogen-free chickens inoculated with inactivated FAdV-1, FAdV-4, and FAdV-8a. The assay showed high sensitivities for all three detected serotypes within FAdV-I. In conclusion, a novel, common ELISA for FAdV-I was developed in this study and could be a powerful tool for seroepidemiological investigations and FAdVs vaccine development.
has issue date	2019-12-14(xsd:dateTime)
bibo:doi	10.1007/s00253-019-10208-3
bibo:pmid	31836910
has license	no-cc
sha1sum (hex)	9519460b4cbca41d693eb60137dac3a6e825fc61
schema:url	https://doi.org/10.1007/s00253-019-10208-3
resource representing a document's title	Development and application of a novel ELISA for detecting antibodies against group I fowl adenoviruses
has PubMed Central identifier	PMC7223807
has PubMed identifier	31836910
schema:publication	Appl Microbiol Biotechnol
resource representing a document's body	covid:9519460b4cbca41d693eb60137dac3a6e825fc61#body_text
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