About: Self-Oligomerization Is Essential for Enhanced Immunological Activities of Soluble Recombinant Calreticulin

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Self-Oligomerization Is Essential for Enhanced Immunological Activities of Soluble Recombinant Calreticulin Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Self-Oligomerization Is Essential for Enhanced Immunological Activities of Soluble Recombinant Calreticulin
Creator	Gao, Xiao-Ming Duo, Cui-Cui Gong, Fang-Yuan Gong, Zheng Guo, Bing-Nan Hong, Chao Huang, Shang-Hui Xiong, Si-Dong Zhang, Li-Juan Zhao, Li-Xiang
Source	PMC
abstract	We have recently reported that calreticulin (CRT), a luminal resident protein, can be found in the sera of patients with rheumatoid arthritis and also that recombinant CRT (rCRT) exhibits extraordinarily strong immunological activities. We herein further demonstrate that rCRT fragments 18–412 (rCRT/18-412), rCRT/39-272, rCRT/120-308 and rCRT/120-250 can self-oligomerize in solution and are 50–100 fold more potent than native CRT (nCRT, isolated from mouse livers) in activating macrophages in vitro. We narrowed down the active site of CRT to residues 150–230, the activity of which also depends on dimerization. By contrast, rCRT/18-197 is almost completely inactive. When rCRT/18-412 is fractionated into oligomers and monomers by gel filtration, the oligomers maintain most of their immunological activities in terms of activating macrophages in vitro and inducing specific antibodies in vivo, while the monomers were much less active by comparison. Additionally, rCRT/18-412 oligomers are much better than monomers in binding to, and uptake by, macrophages. Inhibition of macrophage endocytosis partially blocks the stimulatory effect of rCRT/18-412. We conclude that the immunologically active site of CRT maps between residues 198–230 and that soluble CRT could acquire potent immuno-pathological activities in microenvironments favoring its oligomerization.
has issue date	2013-06-10(xsd:dateTime)
bibo:doi	10.1371/journal.pone.0064951
bibo:pmid	23762269
has license	cc-by
sha1sum (hex)	955cb107f58aff48a58de2473c2fe04e42bfa1e3
schema:url	https://doi.org/10.1371/journal.pone.0064951
resource representing a document's title	Self-Oligomerization Is Essential for Enhanced Immunological Activities of Soluble Recombinant Calreticulin
has PubMed Central identifier	PMC3677884
has PubMed identifier	23762269
schema:publication	PLoS One
resource representing a document's body	covid:955cb107f58aff48a58de2473c2fe04e42bfa1e3#body_text
is schema:about of	named entity 'BASIC RESEARCH' named entity 'macrophages' named entity 'activities' named entity 'potent' named entity 'depends' named entity 'sera' named entity 'terms' named entity 'oligomers' named entity 'livers' named entity 'immunologically' named entity 'Jiangsu Province' named entity 'Foundation' named entity 'SCIENCE' named entity 'EFFECT' named entity 'GEL FILTRATION' named entity 'MUCH BETTER' named entity 'CRT' named entity 'ACTIVE' named entity 'CONTRAST' named entity 'THEIR' named entity 'LUMINAL' named entity 'ACTIVITIES' named entity 'SOLUTION' named entity 'COMPLETELY' named entity '150' named entity 'IMMUNOLOGICAL' named entity 'ACTIVATING' named entity 'RHEUMATOID ARTHRITIS' named entity 'MAINTAIN' named entity 'COMPARISON' named entity 'INACTIVE' named entity '412' named entity 'MUCH' named entity 'PATIENTS' named entity 'BINDING' named entity 'INHIBITION' named entity 'MACROPHAGES' named entity 'MONOMERS' named entity '100' named entity '250' named entity 'SOLUBLE' named entity 'IN VIVO' named entity 'IN VITRO' named entity 'PROTEIN' named entity 'ANTIBODIES' named entity 'FOUNDATION' named entity 'NATIONAL' named entity '308' named entity 'HAVE' named entity 'ACQUIRE' named entity 'TERMS' named entity 'BLOCKS' named entity 'ACTIVE SITE' named entity 'MOST OF' named entity 'RECENTLY' named entity 'FOLD' named entity 'STRONG' named entity 'FAVORING' named entity 'SITE OF' named entity 'HIGHER EDUCATION' named entity 'JIANGSU PROVINCE' named entity 'ACADEMIC' named entity 'NATURE' named entity 'ITS' named entity 'KEY' named entity 'PROGRAMS' named entity 'PRIORITY' named entity 'SERA' named entity 'SPECIFIC' named entity 'CALRETICULIN' named entity 'OLIGOMERIZATION' named entity 'EXHIBITS'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software