About: Long-Term Culture of Distal Airway Epithelial Cells Allows Differentiation Towards Alveolar Epithelial Cells Suited for Influenza Virus Studies

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About: Long-Term Culture of Distal Airway Epithelial Cells Allows Differentiation Towards Alveolar Epithelial Cells Suited for Influenza Virus Studies Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Long-Term Culture of Distal Airway Epithelial Cells Allows Differentiation Towards Alveolar Epithelial Cells Suited for Influenza Virus Studies
Creator	Hocke, Andreas Karlas, Alexander Meyer, Thomas Berger, Hilmar Imai, Seiichiro Imai-Matsushima, Aki Martin-Sancho, Laura Mollenkopf, Hans-Joachim Zoranovic, Tamara
topic	covid:95932152552726907534123bae9e9c61eec15d02#this
source	PMC
abstract	As the target organ for numerous pathogens, the lung epithelium exerts critical functions in health and disease. However, research in this area has been hampered by the quiescence of the alveolar epithelium under standard culture conditions. Here, we used human distal airway epithelial cells (DAECs) to generate alveolar epithelial cells. Long-term, robust growth of human DAECs was achieved using co-culture with feeder cells and supplementation with epidermal growth factor (EGF), Rho-associated protein kinase inhibitor Y27632, and the Notch pathway inhibitor dibenzazepine (DBZ). Removal of feeders and priming with DBZ and a cocktail of lung maturation factors prevented the spontaneous differentiation into airway club cells and instead induced differentiation to alveolar epithelial cells. We successfully transferred this approach to chicken distal airway cells, thus generating a zoonotic infection model that enables studies on influenza A virus replication. These cells are also amenable for gene knockdown using RNAi technology, indicating the suitability of the model for mechanistic studies into lung function and disease.
has issue date	2018-06-22(xsd:dateTime)
bibo:doi	10.1016/j.ebiom.2018.05.032
bibo:pmid	29937069
has license	cc-by-nc-nd
sha1sum (hex)	95932152552726907534123bae9e9c61eec15d02
schema:url	https://doi.org/10.1016/j.ebiom.2018.05.032
resource representing a document's title	Long-Term Culture of Distal Airway Epithelial Cells Allows Differentiation Towards Alveolar Epithelial Cells Suited for Influenza Virus Studies
has PubMed Central identifier	PMC6085545
has PubMed identifier	29937069
schema:publication	EBioMedicine
resource representing a document's body	covid:95932152552726907534123bae9e9c61eec15d02#body_text
is http://vocab.deri.ie/void#inDataset of	proxy:http/ns.inria.fr/covid19/95932152552726907534123bae9e9c61eec15d02
is schema:about of	named entity 'DIFFERENTIATION' named entity 'ALVEOLAR EPITHELIAL CELLS' named entity 'DBZ' named entity 'FUNCTIONS' named entity 'PATHWAY INHIBITOR' named entity 'CRITICAL' named entity 'USED' named entity 'VIRUS REPLICATION' named entity 'HEALTH AND DISEASE' named entity 'REMOVAL' named entity 'INDICATING' named entity 'DISEASE' named entity 'SPONTANEOUS' named entity 'LUNG FUNCTION' named entity 'GENERATE' named entity 'STUDIES' named entity 'RNAI' named entity 'CLUB' named entity 'HUMAN' named entity 'AIRWAY' named entity 'LONG-TERM' named entity 'GENE KNOCKDOWN' named entity 'growth factor' named entity 'mechanistic' named entity 'license' named entity 'STUDIES' named entity 'LONG-TERM CULTURE' named entity 'ALVEOLAR EPITHELIAL CELLS' named entity 'THESE' named entity 'CO-CULTURE' named entity 'INFLUENZA A VIRUS' named entity 'EXERTS' named entity 'ZOONOTIC INFECTION' named entity 'LICENSE' named entity 'LUNG EPITHELIUM' named entity 'FEEDER CELLS' named entity 'EPIDERMAL GROWTH FACTOR' named entity 'CELLS' named entity 'ENABLES' named entity 'CONDITIONS' named entity 'ALLOWS' named entity 'EPITHELIAL CELLS' named entity 'ORG' named entity 'DISTAL' named entity 'NC-' named entity 'NOTCH' named entity 'MATURATION' named entity 'MODEL' named entity 'SUPPLEMENTATION' named entity 'NUMEROUS' named entity 'TARGET ORGAN' named entity 'LUNG' named entity 'DIBENZAZEPINE' named entity 'GENERATING' named entity 'COCKTAIL' named entity 'RHO-ASSOCIATED PROTEIN KINASE INHIBITOR' named entity 'STANDARD' named entity 'TECHNOLOGY' named entity 'PRIMING' named entity 'GROWTH' named entity 'HERE' named entity 'PATHOGENS' named entity 'QUIESCENCE' named entity 'EPITHELIAL CELLS' named entity 'MECHANISTIC STUDIES' named entity 'AIRWAY' named entity 'INFLUENZA VIRUS' named entity 'DISTAL' named entity 'AMENABLE' named entity 'DIFFERENTIATION' named entity 'ROBUST'

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