About: The SARS Coronavirus E Protein Interacts with PALS1 and Alters Tight Junction Formation and Epithelial Morphogenesis

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	The SARS Coronavirus E Protein Interacts with PALS1 and Alters Tight Junction Formation and Epithelial Morphogenesis
Creator	Bruzzone, Roberto Nal, Béatrice Chan, Wing-Lim Liu, Chia-Jen Margolis, Benjamin Siu, Yu-Lam Teoh, Kim-Tat Peiris, J. Schlüter, Marc Nusrat, Asma
Source	Medline; PMC
abstract	Intercellular tight junctions define epithelial apicobasal polarity and form a physical fence which protects underlying tissues from pathogen invasions. PALS1, a tight junction-associated protein, is a member of the CRUMBS3-PALS1-PATJ polarity complex, which is crucial for the establishment and maintenance of epithelial polarity in mammals. Here we report that the carboxy-terminal domain of the SARS-CoV E small envelope protein (E) binds to human PALS1. Using coimmunoprecipitation and pull-down assays, we show that E interacts with PALS1 in mammalian cells and further demonstrate that the last four carboxy-terminal amino acids of E form a novel PDZ-binding motif that binds to PALS1 PDZ domain. PALS1 redistributes to the ERGIC/Golgi region, where E accumulates, in SARS-CoV–infected Vero E6 cells. Ectopic expression of E in MDCKII epithelial cells significantly alters cyst morphogenesis and, furthermore, delays formation of tight junctions, affects polarity, and modifies the subcellular distribution of PALS1, in a PDZ-binding motif-dependent manner. We speculate that hijacking of PALS1 by SARS-CoV E plays a determinant role in the disruption of the lung epithelium in SARS patients.
has issue date	2010-11-15(xsd:dateTime)
bibo:doi	10.1091/mbc.e10-04-0338
bibo:pmid	20861307
has license	cc-by-nc-sa
schema:url	https://doi.org/10.1091/mbc.e10-04-0338
resource representing a document's title	The SARS Coronavirus E Protein Interacts with PALS1 and Alters Tight Junction Formation and Epithelial Morphogenesis
has PubMed Central identifier	PMC2982091
has PubMed identifier	20861307
schema:publication	Mol Biol Cell
resource representing a document's body	covid:PMC2982091#body_text
is schema:about of	named entity 'Junction' named entity 'Tight Junction' named entity 'Epithelial' named entity 'GST' named entity 'protein' named entity 'Millipore' named entity 'morphogenesis' named entity 'peptides' named entity 'E-cadherin' named entity 'anti-Flag' named entity 'lumen' named entity 'West Grove' named entity 'primary antibodies' named entity 'EGFP' named entity 'antibodies' named entity 'phenotypes' named entity 'Gibbstown' named entity 'PDZ domain' named entity 'infection' named entity 'PBS' named entity 'Billerica' named entity 'nonspecific binding' named entity 'lung' named entity 'cell lysates' named entity 'epithelial cells' named entity 'Carlsbad, CA' named entity 'amino acids' named entity 'PDZ' named entity 'Invitrogen' named entity 'protein' named entity 'plasmid vectors' named entity 'infection' named entity 'peroxidase' named entity 'S.A.' named entity 'GST' named entity 'interact' named entity 'amino acids' named entity 'Golgi' named entity 'Tris-HCl' named entity 'systemic circulation' named entity 'peptide' named entity 'monoclonal antibodies' named entity 'cDNA' named entity 'cell-cell contacts' named entity 'fusion proteins' named entity 'Covance' named entity 'virulence factor' named entity 'carboxy-terminal tail' named entity 'retroviral vector' named entity 'CRB' named entity 'Golgi' named entity 'goat' named entity 'gag-pol' named entity 'clones' named entity 'protein' named entity 'perinuclear' named entity 'cell lysis' named entity 'Vero E6' named entity 'Alexa Fluor' named entity 'pneumocytes' named entity 'low calcium' named entity 'gene' named entity 'intracellular trafficking' named entity 'clones' named entity 'CRB1' named entity 'Figure 8' named entity 'extrapulmonary' named entity 'SARS-CoV' named entity 'University of Hong Kong' named entity 'cytosolic' named entity 'GST' named entity 'PDZ' named entity 'cyst'

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