About: An integrated microfluidic system for rapid diagnosis of dengue virus infection

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: An integrated microfluidic system for rapid diagnosis of dengue virus infection Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	An integrated microfluidic system for rapid diagnosis of dengue virus infection
Creator	Lee, Gwo-Bin Lei, Huan-Yao Lien, Kang-Yi Lee, Yu-Fang
Source	Elsevier; Medline; PMC
abstract	This study reports an integrated microfluidic system which utilizes virus-bound magnetic bead complexes for rapid serological analysis of antibodies associated with an infection by the dengue virus. This new microfluidic system integrates one-way micropumps, a four-membrane-type micromixer, two-way micropumps and an on-chip microcoil array in order to simultaneously perform the rapid detection of immunoglobulin G (IgG) and immunoglobulin M (IgM). An IgM/IgG titer in serum is used to confirm the presence of dengue virus infection. By utilizing microfluidic technologies and virus-bound magnetic beads, IgG and IgM in the serum samples are captured. This is followed by purification and isolation of these beads utilizing a magnetic field generated from the on-chip array of microcoils. Any interfering substances in the biological fluids are washed away automatically by the flow generated by the integrated pneumatic pumps. The fluorescence-labelled secondary antibodies are bound to the surface of the IgG/IgM complex attached onto the magnetic beads. Finally, the entire magnetic complex sandwich is transported automatically into a sample detection chamber. The optical signals are then measured and analyzed by a real-time optical detection module. The entire process is performed automatically on a single chip within 30 min, which is only 1/8th of the time required for a traditional method. More importantly, the detection limit has been improved to 21 pg, which is about 38 times better when compared to traditional methods. This integrated system may provide a powerful platform for the rapid diagnosis of dengue virus infection and other types of infectious diseases.
has issue date	2009-12-15(xsd:dateTime)
bibo:doi	10.1016/j.bios.2009.08.020
bibo:pmid	19744849
has license	no-cc
schema:url	https://doi.org/10.1016/j.bios.2009.08.020
resource representing a document's title	An integrated microfluidic system for rapid diagnosis of dengue virus infection
has PubMed Central identifier	PMC7125828
has PubMed identifier	19744849
schema:publication	Biosens Bioelectron
resource representing a document's body	covid:PMC7125828#body_text
is schema:about of	named entity 'integrated' named entity 'rapid' named entity 'virus' named entity 'dengue fever' named entity 'magnetic bead-based' named entity 'pathogens' named entity 'immunoassays' named entity 'Auroux' named entity 'compressed air' named entity 'public health' named entity 'IgM' named entity 'antibodies and antigens' named entity 'micromixer' named entity 'fluorescent intensity' named entity 'dengue virus' named entity 'immunoassay' named entity 'IgM' named entity 'detection limit' named entity 'IgG' named entity 'PDMS' named entity 'immunoassay' named entity 'microfluidic' named entity 'IgG' named entity 'PDMS' named entity 'IgG' named entity 'microfluidic' named entity 'IgG' named entity 'microfluidic' named entity 'Germany' named entity 'antibodies' named entity 'FITC' named entity 'dengue virus' named entity 'polymer' named entity 'viral particles' named entity 'fluorescent intensity' named entity 'EV71' named entity 'IgM' named entity 'epitopes' named entity 'direct current' named entity 'charge-coupled device' named entity 'serum' named entity 'polyclonal' named entity 'Hapugoda' named entity 'immunoassay' named entity 'PMT' named entity 'antibody-conjugated' named entity 'magnetic bead-based' named entity 'IgM' named entity 'National Cheng Kung University' named entity 'antibodies' named entity 'Mikrotron' named entity 'antibody-conjugated' named entity 'IgG' named entity 'dengue' named entity 'IgG' named entity 'micromixer' named entity 'phosphate' named entity 'immunoglobulin' named entity 'Taiwan' named entity 'dengue' named entity 'FITC' named entity 'antibody' named entity 'IgM' named entity 'virus' named entity 'IgG' named entity 'immunoassay' named entity 'IgG' named entity 'compressed air' named entity 'micromixer' named entity 'Dow Corning' named entity 'Taipei' named entity 'antigen' named entity 'virus' named entity '4.02' named entity 'dengue' named entity 'serum samples' named entity 'photo-multiplier tube' named entity 'IgG' named entity 'IgG'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software