About: LUCS: a high-resolution nucleic acid sequencing tool for accurate long-read analysis of individual DNA molecules

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About: LUCS: a high-resolution nucleic acid sequencing tool for accurate long-read analysis of individual DNA molecules Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	LUCS: a high-resolution nucleic acid sequencing tool for accurate long-read analysis of individual DNA molecules
Creator	Annis, Sofia Fleischmann, Zoë Franco, Melissa Khrapko, Konstantin Logan, Robert Mullin-Bernstein, Zachary Saürich, Josefin Tilly, Jonathan Woods, Dori
Source	Medline; PMC
abstract	Nucleic acid sequence analyses are fundamental to all aspects of biological research, spanning aging, mitochondrial DNA (mtDNA) and cancer, as well as microbial and viral evolution. Over the past several years, significant improvements in DNA sequencing, including consensus sequence analysis, have proven invaluable for high-throughput studies. However, all current DNA sequencing platforms have limited utility for studies of complex mixtures or of individual long molecules, the latter of which is crucial to understanding evolution and consequences of single nucleotide variants and their combinations. Here we report a new technology termed LUCS (Long-molecule UMI-driven Consensus Sequencing), in which reads from third-generation sequencing are aggregated by unique molecular identifiers (UMIs) specific for each individual DNA molecule. This enables in-silico reconstruction of highly accurate consensus reads of each DNA molecule independent of other molecules in the sample. Additionally, use of two UMIs enables detection of artificial recombinants (chimeras). As proof of concept, we show that application of LUCS to assessment of mitochondrial genomes in complex mixtures from single cells was associated with an error rate of 1X10(-4) errors/nucleotide. Thus, LUCS represents a major step forward in DNA sequencing that offers high-throughput capacity and high-accuracy reads in studies of long DNA templates and nucleotide variants in heterogenous samples.
has issue date	2020-04-28(xsd:dateTime)
bibo:doi	10.18632/aging.103171
bibo:pmid	32345770
has license	cc-by
sha1sum (hex)	a3e287fee5b0f32d1c3587dfcd1a752a6c09bd2d
schema:url	https://doi.org/10.18632/aging.103171
resource representing a document's title	LUCS: a high-resolution nucleic acid sequencing tool for accurate long-read analysis of individual DNA molecules
has PubMed Central identifier	PMC7202536
has PubMed identifier	32345770
schema:publication	Aging (Albany NY)
resource representing a document's body	covid:a3e287fee5b0f32d1c3587dfcd1a752a6c09bd2d#body_text
is schema:about of	named entity 'step' named entity 'reconstruction' named entity 'mitochondrial genomes' named entity 'evolution' named entity 'MOLECULE' named entity 'NUCLEIC ACID SEQUENCE' named entity 'DETECTION' named entity 'A MAJOR' named entity 'PROOF OF CONCEPT' covid:arg/a3e287fee5b0f32d1c3587dfcd1a752a6c09bd2d named entity 'DNA sequencing' named entity 'studies' named entity 'DNA' named entity 'termed' named entity 'platforms' named entity 'proven' named entity 'DNA' named entity 'insilico' named entity 'DNA sequencing' named entity 'molecule' named entity 'DNA molecules' named entity 'Student Exchange Program' named entity 'Sequencing' named entity '2, 3' named entity 'polymorphic sites' named entity 'living organisms' named entity 'genetic variants' named entity 'DNA amplification' named entity 'entire genomes' named entity 'guanines' named entity 'mutation rate' named entity 'UMIs' named entity 'primers' named entity 'UMIs' named entity 'molecule' named entity 'medaka' named entity 'molecule' named entity 'DNA genome' named entity 'mitochondrial genome' named entity '95%' named entity 'sequencing technologies' named entity 'UMIs' named entity 'single-molecule' named entity 'polymorphic variants' named entity 'single-molecule sequencing' named entity 'nucleotides' named entity 'DNA fragments' named entity 'heteroplasmic' named entity 'fluorescent probes' named entity 'methylated' named entity 'transversions' named entity 'UMIs' named entity 'mtDNA' named entity 'Sanger' named entity 'cloning' named entity 'centroid' named entity 'single nucleotide variants' named entity 'PCR amplification' named entity 'PCR' named entity 'fast5' named entity 'amino acid' named entity 'diagnostic procedures' named entity 'mtDNA' named entity 'PacBio SMRT' named entity 'Polg' named entity 'evolution' named entity 'polymorphic' named entity 'PCR' named entity 'non-living' named entity 'ultrapure water' named entity 'PacBio' named entity 'SNVs' named entity 'primers'

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