About: Interactome Profile of the Host Cellular Proteins and the Nonstructural Protein 2 of Porcine Reproductive and Respiratory Syndrome Virus

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About: Interactome Profile of the Host Cellular Proteins and the Nonstructural Protein 2 of Porcine Reproductive and Respiratory Syndrome Virus Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Interactome Profile of the Host Cellular Proteins and the Nonstructural Protein 2 of Porcine Reproductive and Respiratory Syndrome Virus
Creator	Wang, Li Zhou, Lei Li, Yan Zhang, Han Ge, Xinna Guo, Xin Yang, Hanchun Yu, Kangzhen
Source	PMC
abstract	The nonstructural protein 2 (NSP2) is considered to be one of crucial viral proteins in the replication and pathogenesis of porcine reproductive and respiratory syndrome virus (PRRSV). In the present study, the host cellular proteins that interact with the NSP2 of PRRSV were immunoprecipitated with anti-Myc antibody from the MARC-145 cells infected by a recombinant PRRSV with 3xMyc tag insertion in its NSP2-coding region, and then 285 cellular proteins interacting with NSP2 were identified by LC-MS/MS. The Gene Ontology and enriched KEGG Pathway bioinformatics analyses indicated that the identified proteins could be assigned to different subcellular locations and functional classes. Functional analysis of the interactome profile highlighted cellular pathways associated with infectious disease, translation, immune system, nervous system and signal transduction. Two interested cellular proteins–BCL2-associated athanogene 6 (BAG6) and apoptosis-inducing factor 1 (AIF1) which may involve in transporting of NSP2 to Endoplasmic reticulum (ER) or PRRSV-driven apoptosis were validated by Western blot. The interactome data between PRRSV NSP2 and cellular proteins contribute to the understanding of the roles of NSP2 in the replication and pathogenesis of PRRSV, and also provide novel cellular target proteins for elucidating the associated molecular mechanisms of the interaction of host cellular proteins with viral proteins in regulating the viral replication.
has issue date	2014-06-05(xsd:dateTime)
bibo:doi	10.1371/journal.pone.0099176
bibo:pmid	24901321
has license	cc-by
sha1sum (hex)	a4995af42a012dd1b7f8da3936d79d78d0e24405
schema:url	https://doi.org/10.1371/journal.pone.0099176
resource representing a document's title	Interactome Profile of the Host Cellular Proteins and the Nonstructural Protein 2 of Porcine Reproductive and Respiratory Syndrome Virus
has PubMed Central identifier	PMC4047090
has PubMed identifier	24901321
schema:publication	PLoS One
resource representing a document's body	covid:a4995af42a012dd1b7f8da3936d79d78d0e24405#body_text
is schema:about of	named entity 'infectious disease' named entity 'study' named entity 'Functional analysis' named entity 'cellular' named entity 'target' named entity 'host' named entity 'interacting' named entity 'Funds' named entity 'project' covid:arg/a4995af42a012dd1b7f8da3936d79d78d0e24405 named entity 'respiratory' named entity 'proteins' named entity 'apoptosis' named entity 'viral' named entity 'provide' named entity 'interact' named entity 'interactome' named entity 'coding region' named entity 'infected' named entity 'tag' named entity 'assigned' named entity 'viral replication' named entity 'porcine' named entity 'validated' named entity 'interactome' named entity 'pathogenesis' named entity 'porcine reproductive and respiratory syndrome virus' named entity 'NSP2' named entity 'KEGG' named entity 'signal transduction' named entity 'Endoplasmic reticulum' named entity 'infectious disease' named entity 'nonstructural protein' named entity 'RNAs' named entity 'Myc' named entity 'PRRSV' named entity 'virus' named entity 'AIF1' named entity 'monoclonal antibody' named entity 'signaling pathway' named entity 'viral pathogenesis' named entity 'growth kinetics' named entity 'bioinformatics' named entity 'Jiangsu' named entity 'Germany' named entity 'IgG' named entity 'positive sense RNA' named entity 'human embryonic' named entity 'cell growth' named entity 'PRRSV' named entity 'KEGG' named entity 'confocal microscopy' named entity 'sequence analysis' named entity 'AIF1' named entity 'alkylation' named entity 'vector' named entity 'NSP2' named entity 'NSP2' named entity 'NSP2' named entity 'vector' named entity 'PCR' named entity 'KEGG' named entity 'plasmid' named entity 'MOI' named entity 'NSP2' named entity 'immune response' named entity 'MOI' named entity 'RT-PCR' named entity 'NSP2' named entity 'virus' named entity 'cell lysates' named entity 'mitochondrial' named entity 'PRRSV' named entity 'PRRSV'

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