About: Absolute quantitation of infectious salmon anaemia virus using different real-time reverse transcription PCR chemistries

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Absolute quantitation of infectious salmon anaemia virus using different real-time reverse transcription PCR chemistries Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Absolute quantitation of infectious salmon anaemia virus using different real-time reverse transcription PCR chemistries
Creator	Kibenge, Frederick Iwamoto, Tokinori Kibenge, Molly Workenhe, Samuel
source	Elsevier; Medline; PMC
abstract	Routine laboratory diagnosis of infectious salmon anaemia virus (ISAV) infection is primarily by reverse transcription polymerase chain reaction (RT-PCR) because of the high sensitivity and rapid turnaround time of the test. This paper describes methods for highly reproducible absolute viral load measurements using external standard curves generated with either ISAV recombinant plasmid DNA (pDNA) standards or transcribed RNA standards prepared by in vitro transcription with T7 RNA polymerase, and using a two tube real-time or quantitative (q)RT-PCR with SYBR(®) Green I chemistry and a single tube qRT-PCR with TaqMan(®) probe chemistry. When applied to virus samples of known virus titer for the highly pathogenic ISAV strain NBISA01 and the low pathogenic ISAV strain RPC/NB-04-085-1, both methods showed a 100-fold lower detectable titer for RPC/NB-04-085-1 but with a higher number of viral RNA molecules compared to NBISA01. Overall, the SYBR(®) Green I method overestimated copy numbers in samples having equivalent Ct values with the TaqMan(®) probe method. Taken together, the findings suggest that the TaqMan(®) probe method with the in vitro transcribed RNA standard curve is the preferred method for reliable and rapid quantitation of ISAV in samples.
has issue date	2008-09-26(xsd:dateTime)
bibo:doi	10.1016/j.jviromet.2008.08.007
bibo:pmid	18789975
has license	no-cc
sha1sum (hex)	aa8b246a6ace4ef2784775bae44250f4ac54aa0e
schema:url	https://doi.org/10.1016/j.jviromet.2008.08.007
resource representing a document's title	Absolute quantitation of infectious salmon anaemia virus using different real-time reverse transcription PCR chemistries
has PubMed Central identifier	PMC7112891
has PubMed identifier	18789975
schema:publication	J Virol Methods
resource representing a document's body	covid:aa8b246a6ace4ef2784775bae44250f4ac54aa0e#body_text
is schema:about of	named entity 'ABSOLUTE QUANTITATION' named entity 'tube' named entity 'TaqMan' named entity 'detectable' named entity 'Routine' named entity 'compared' named entity 'transcribed' named entity 'strain' named entity 'curves' named entity 'infectious salmon anaemia' named entity 'quantitation' named entity 'KNOWN' named entity 'PROBE' named entity 'CHEMISTRIES' named entity 'USING' named entity 'REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION' named entity 'T7 RNA POLYMERASE' named entity 'TEST' named entity 'COPY' named entity 'INFECTIOUS SALMON ANAEMIA VIRUS' named entity 'PLASMID DNA' named entity 'METHODS' named entity 'FOLD' covid:arg/aa8b246a6ace4ef2784775bae44250f4ac54aa0e named entity 'TUBE' named entity 'QUANTITATIVE' named entity 'RECOMBINANT PLASMID' named entity 'GENERATED' named entity 'FINDINGS' named entity 'STRAIN' named entity 'OVERALL' named entity 'IN VITRO TRANSCRIPTION' named entity 'EXTERNAL' named entity 'PCR' named entity 'SAMPLES' named entity 'RNA' named entity 'INFECTIOUS SALMON ANAEMIA VIRUS' named entity 'REAL-TIME' named entity 'REVERSE TRANSCRIPTION PCR' named entity 'USING' named entity 'DIFFERENT' named entity 'TITER' named entity 'HIGHLY' named entity 'CHEMISTRY' named entity 'QRT-PCR' named entity 'COMPARED' named entity 'PATHOGENIC' named entity 'ROUTINE' named entity 'TIME' named entity 'MEASUREMENTS' named entity 'TAQMAN' named entity 'GREEN ' named entity 'LABORATORY DIAGNOSIS' named entity 'SUGGEST' named entity 'PREPARED' named entity 'RELIABLE' named entity 'VALUES' named entity '100' named entity 'APPLIED' named entity 'TRANSCRIBED' named entity 'EQUIVALENT' named entity 'STANDARDS' named entity 'DETECTABLE' named entity 'LOW' named entity 'MOLECULES' named entity 'VIRUS' named entity 'RAPID' named entity 'IN VITRO' named entity 'VIRAL LOAD' named entity 'PAPER' named entity 'QUANTITATION' named entity 'CURVE' named entity 'STANDARD' named entity 'RPC' named entity 'VIRAL RNA' named entity 'METHOD' named entity 'INFECTION' named entity 'HIGH SENSITIVITY'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2025 OpenLink Software