About: Comparative studies of the capsid precursor polypeptide P1 and the capsid protein VP1 cDNA vectors for DNA vaccination against foot‐and‐mouth disease virus

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About: Comparative studies of the capsid precursor polypeptide P1 and the capsid protein VP1 cDNA vectors for DNA vaccination against foot‐and‐mouth disease virus Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Comparative studies of the capsid precursor polypeptide P1 and the capsid protein VP1 cDNA vectors for DNA vaccination against foot‐and‐mouth disease virus
Creator	Yang, Ning-Sun Jong, Ming-Hwa Cheng, R Chan, Ming-Tsair Kim, Suk-Am Kuo, Tsun-Yung Lai, Shiow-Suey Liang, Shu-Mei Lin, Ku-Feng Wang, Chien-Yu Wang, Jeng-Hwan Yang, Yu-Ling
Source	Medline; PMC
abstract	BACKGROUND: Foot‐and‐mouth disease virus (FMDV) causes a severe livestock disease, and the virus is an interesting target for virology and vaccine studies. MATERIALS AND METHODS: Here we evaluated comparatively three different viral antigen‐encoding DNA sequences, delivered via two physical means (i.e., gene gun delivery into skin and electroporation delivery into muscle), for naked DNA‐mediated vaccination in a mouse system. RESULTS: Both methods gave similar results, demonstrating commonality of the observed DNA vaccine effects. Immunization with a cDNA vector expressing the major viral antigen (VP1) alone routinely failed to induce the production of anti‐VP1 or neutralizing antibodies in test mice. As a second approach, the plasmid L‐VP1 that produces a transgenic membrane‐anchored VP1 protein elicited a strong antibody response, but all test mice failed in the FMDV challenge experiment. In contrast, for mice immunized with the viral capsid precursor protein (P1) cDNA expression vector, both neutralizing antibodies and 80–100% protection in test mice were detected. CONCLUSIONS: This strategy of using the whole capsid precursor protein P1 cDNA for vaccination, intentionally without the use of virus‐specific protease or other encoding genes for safety reasons, may thus be employed as a relevant experimental system for induction or upgrading of effective neutralizing antibody response, and as a convenient surrogate test system for DNA vaccination studies of FMDV and presumably other viral diseases. Copyright © 2005 John Wiley & Sons, Ltd.
has issue date	2005-02-03(xsd:dateTime)
bibo:doi	10.1002/jgm.723
bibo:pmid	15693054
has license	no-cc
sha1sum (hex)	ad499c94d157b5cd65d65547361ef905fa8d7977
schema:url	https://doi.org/10.1002/jgm.723
resource representing a document's title	Comparative studies of the capsid precursor polypeptide P1 and the capsid protein VP1 cDNA vectors for DNA vaccination against foot‐and‐mouth disease virus
has PubMed Central identifier	PMC7166641
has PubMed identifier	15693054
schema:publication	J Gene Med
resource representing a document's body	covid:ad499c94d157b5cd65d65547361ef905fa8d7977#body_text
is schema:about of	named entity 'CAUSES' named entity 'livestock disease' named entity 'virus' named entity 'capsid protein VP1' named entity 'DNA vaccination' named entity 'cDNA' named entity 'gene gun' named entity 'protein folding' named entity 'gene' named entity 'blood samples' named entity 'helium' named entity 'culture medium' named entity 'transfection' named entity 'host cell' named entity 'CMV' named entity 'virus' named entity 'VP-4' named entity 'serotype' named entity 'horse serum' named entity 'immunization' named entity 'vaccine' named entity 'optical density' named entity 'VP3' named entity 'PBS' named entity 'pseudorabies virus' named entity 'virus particle' named entity 'FMDV' named entity 'Th1' named entity 'gene' named entity 'mice' named entity 'tibialis anterior' named entity 'mice' named entity 'protein' named entity 'virus' named entity 'muscle' named entity 'transgene' named entity 'polyprotein' named entity 'antibodies' named entity 'DNA vaccine' named entity 'protein' named entity 'BALB/c' named entity 'PBS' named entity 'SARS' named entity 'cloning' named entity 'transgenic' named entity 'neutralizing antibodies' named entity 'gene gun' named entity 'T-cell' named entity 'protein' named entity 'FMDV' named entity 'virus' named entity 'VP1' named entity 'incubation' named entity 'transgenic' named entity 'fetal calf serum' named entity 'CMV promoter' named entity 'VP1' named entity 'fluorescein isothiocyanate' named entity 'BALB/c' named entity 'protein expression' named entity 'transgenic' named entity 'virus' named entity 'FMDV' named entity 'FMDV' named entity 'immune response' named entity 'transgenic' named entity 'FMD' named entity 'muscle tissues' named entity 'recombinant protein' named entity 'mice'

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