About: Evaluation of mouse enteroids as a model for Lawsonia intracellularis infection

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About: Evaluation of mouse enteroids as a model for Lawsonia intracellularis infection Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Evaluation of mouse enteroids as a model for Lawsonia intracellularis infection
Creator	Gebhart, Connie Resende, Talita Vannucci, Fabio Guo, Yue Saqui-Salces, Milena Lekha Medida, Ramya
Source	Medline; PMC
abstract	Lawsonia intracellularis, an obligate intracellular bacterium, is an important enteric pathogen in pig herds and horse farms worldwide. The hallmark feature of L. intracellularis infection is the proliferation of epithelial cells in intestinal crypts. A major limitation to the study of L. intracellularis infection is the lack of an in vitro model that reproduces the changes observed in proliferative enteropathy. Here we investigated the suitability of mouse enteroids as a model to study L. intracellularis infection. Mouse enteroids were microinjected with L. intracellularis, filter-sterilized L. intracellularis culture supernatant, or sterile cell culture media (DMEM). L. intracellularis antigen was detected in mouse enteroids by immunohistochemistry and was located mostly in the basal region of the epithelium. There was no differential growth of enteroids among treatment groups, and cellular proliferation was not increased in L. intracellularis-infected enteroids in relation to non-infected enteroids based on immunofluorescence staining. L. intracellularis infection did not induce changes in gene expression of Ki-67 (proliferation marker), Sox9 (marker for transit amplifying cells) and Muc2 (marker for goblet cells). These results indicate that although L. intracellularis antigen is detectable in mouse enteroids, indicating susceptibility to infection, mouse enteroids fail to replicate the cellular proliferation and gene expression changes observed in proliferative enteropathy. Nevertheless, we have successfully demonstrated that mouse enteroids can be used to model days-long intracellular pathogen infection, serving as potential models for the study of other pathogens of interest in veterinary medicine.
has issue date	2019-07-19(xsd:dateTime)
bibo:doi	10.1186/s13567-019-0672-9
bibo:pmid	31324204
has license	cc-by
sha1sum (hex)	ae165f6805cbb0b5c688d4d5e5a89b6b7c16fae0
schema:url	https://doi.org/10.1186/s13567-019-0672-9
resource representing a document's title	Evaluation of mouse enteroids as a model for Lawsonia intracellularis infection
has PubMed Central identifier	PMC6642515
has PubMed identifier	31324204
schema:publication	Vet Res
resource representing a document's body	covid:ae165f6805cbb0b5c688d4d5e5a89b6b7c16fae0#body_text
is schema:about of	named entity 'veterinary medicine' named entity 'worldwide' named entity 'limitation' named entity 'study' named entity 'MODEL' named entity 'MOUSE' named entity 'STERILIZED' named entity 'BASED' named entity 'CELLULAR PROLIFERATION' named entity 'MODELS' named entity 'OBLIGATE' named entity 'PATHOGEN' named entity 'RESULTS' named entity 'LACK' named entity 'INTESTINAL CRYPTS' named entity 'DIFFERENTIAL GROWTH' named entity 'MARKER' named entity 'SERVING' named entity 'MODEL' named entity 'CULTURE SUPERNATANT' named entity 'TRANSIT' named entity 'EPITHELIUM' named entity 'THESE' named entity 'MOUSE' named entity 'PATHOGENS' named entity 'IMMUNOFLUORESCENCE STAINING' named entity 'NON-INFECTED' named entity 'POTENTIAL' named entity 'BASAL REGION' named entity 'SOX9' named entity 'ANTIGEN' named entity 'PROLIFERATIVE' named entity 'ENTEROPATHY' named entity 'CELLS' named entity 'EPITHELIAL CELLS' named entity 'LAWSONIA INTRACELLULARIS' named entity 'A MAJOR' named entity 'VETERINARY MEDICINE' named entity 'RELATION' named entity 'PROLIFERATION' named entity 'HORSE' named entity 'LIMITATION' named entity 'REPLICATE' named entity 'IMMUNOHISTOCHEMISTRY' named entity 'FARMS' named entity 'INFECTION' named entity 'DETECTABLE' named entity 'GENE EXPRESSION' named entity 'EVALUATION' named entity 'GOBLET CELLS' named entity 'IN VITRO MODEL' named entity 'LAWSONIA INTRACELLULARIS' named entity 'INTRACELLULAR' named entity 'IMPORTANT' named entity 'DETECTED' named entity 'MUC2' named entity 'CHANGES' named entity 'STUDY' named entity 'LONG' named entity 'INFECTION' named entity 'HERE' named entity 'SUSCEPTIBILITY TO INFECTION' named entity 'INDUCE' named entity 'PIG' named entity 'INVESTIGATED' named entity 'KI-67' named entity 'DID' named entity 'BACTERIUM' named entity 'STERILE' named entity 'INDICATING' named entity 'INFECTED' named entity 'FAIL' named entity 'GROUPS' named entity 'FEATURE' named entity 'OF INTEREST' named entity 'WORLDWIDE'

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