About: Developmental validation of GlobalFiler™ PCR amplification kit: a 6-dye multiplex assay designed for amplification of casework samples

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About: Developmental validation of GlobalFiler™ PCR amplification kit: a 6-dye multiplex assay designed for amplification of casework samples Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Developmental validation of GlobalFiler™ PCR amplification kit: a 6-dye multiplex assay designed for amplification of casework samples
Creator	Wang, Dennis Zhong, Chang Hennessy, Lori Lagacé, Robert Ludeman, Matthew Mulero, Julio Short, Marc
source	PMC
abstract	The GlobalFiler™ PCR Amplification Kit is a single multiplex assay that amplifies a set of 24 markers, which encompass the European Standard Set and CODIS (Combined DNA Index System) recommended composite set of loci. In addition to more loci and a 6-dye chemistry format, the Master Mix has been formulated to allow higher sample loading volume for trace DNA samples. The GlobalFiler™ Kit has been optimized to deliver high performance on casework samples, while also delivering fast thermal cycling, with an amplification time of approximately 80 min. Here, we report the results of the developmental validation study which followed the SWGDAM (Scientific Working Group on DNA Analysis Methods) guidelines and includes data for PCR-based studies, sensitivity, species specificity, stability, precision, reproducibility and repeatability, concordance, stutter, DNA mixtures, and performance on mock casework samples. The results validate the multiplex design as well as demonstrate the kit’s robustness, reliability, and suitability as an assay for human identification with casework DNA samples. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00414-018-1817-5) contains supplementary material, which is available to authorized users.
has issue date	2018-03-09(xsd:dateTime)
bibo:doi	10.1007/s00414-018-1817-5
bibo:pmid	29523969
has license	no-cc
sha1sum (hex)	af48f1601e0095f91e5c12e868f432be32284abd
schema:url	https://doi.org/10.1007/s00414-018-1817-5
resource representing a document's title	Developmental validation of GlobalFiler™ PCR amplification kit: a 6-dye multiplex assay designed for amplification of casework samples
has PubMed Central identifier	PMC6208722
has PubMed identifier	29523969
schema:publication	Int J Legal Med
resource representing a document's body	covid:af48f1601e0095f91e5c12e868f432be32284abd#body_text
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