About: Structural genomics of the SARS coronavirus: cloning, expression, crystallization and preliminary crystallographic study of the Nsp9 protein

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About: Structural genomics of the SARS coronavirus: cloning, expression, crystallization and preliminary crystallographic study of the Nsp9 protein Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Structural genomics of the SARS coronavirus: cloning, expression, crystallization and preliminary crystallographic study of the Nsp9 protein
Creator	Canard, Bruno Cambillau, Christian Egloff, Marie-Pierre Longhi, Sonia Rancurel, Corinne Snijder, Eric Tocque, Fabienne Dobbe, Jessika Campanacci, Vale Durousseau, Ce Ferron, Franc Salomoni, Aurelia
source	Medline; PMC
abstract	The aetiologic agent of the recent epidemics of Severe Acute Respiratory Syndrome (SARS) is a positive‐stranded RNA virus (SARS‐CoV) belonging to the Coronaviridae family and its genome differs substantially from those of other known coronaviruses. SARS‐CoV is transmissible mainly by the respiratory route and to date there is no vaccine and no prophylactic or therapeutic treatments against this agent. A SARS‐CoV whole‐genome approach has been developed aimed at determining the crystal structure of all of its proteins or domains. These studies are expected to greatly facilitate drug design. The genomes of coronaviruses are between 27 and 31.5 kbp in length, the largest of the known RNA viruses, and encode 20–30 mature proteins. The functions of many of these polypeptides, including the Nsp9–Nsp10 replicase‐cleavage products, are still unknown. Here, the cloning, Escherichia coli expression, purification and crystallization of the SARS‐CoV Nsp9 protein, the first SARS‐CoV protein to be crystallized, are reported. Nsp9 crystals diffract to 2.8 Å resolution and belong to space group P6(1/5)22, with unit‐cell parameters a = b = 89.7, c = 136.7 Å. With two molecules in the asymmetric unit, the solvent content is 60% (V (M) = 3.1 Å(3) Da(−1)).
has issue date	2004-06-07(xsd:dateTime)
bibo:doi	10.1107/s0907444903016779
bibo:pmid	12925794
has license	no-cc
sha1sum (hex)	afc29ba38f925a7d3ed22338b5a9b34f4b30a49c
schema:url	https://doi.org/10.1107/s0907444903016779
resource representing a document's title	Structural genomics of the SARS coronavirus: cloning, expression, crystallization and preliminary crystallographic study of the Nsp9 protein
has PubMed Central identifier	PMC7161644
has PubMed identifier	12925794
schema:publication	Acta Crystallogr D Biol Crystallogr
resource representing a document's body	covid:afc29ba38f925a7d3ed22338b5a9b34f4b30a49c#body_text
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