About: A novel method for the detection of early events in cell-cell fusion of Semliki Forest virus infected cells growing in monolayer cultures

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About: A novel method for the detection of early events in cell-cell fusion of Semliki Forest virus infected cells growing in monolayer cultures Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	A novel method for the detection of early events in cell-cell fusion of Semliki Forest virus infected cells growing in monolayer cultures
Creator	Kempf, C Koblet, H Kohler, U Michel, M
source	PMC
abstract	Semliki Forest virus infected Aedes albopictus cells were used to investigate virus induced cell-cell fusion. It was shown by a novel method that cell-cell fusion was completed within approximately 5 minutes after triggering the fusion event by low pH. This method consists of fixing fusing cells with glutaraldehyde and microinjecting the highly fluroescent and rapidly diffusing dye Lucifer yellow. In contrast, polykaryon formation, the usually used criterion to measure cell-cell fusion occurred only within 30 minutes. Furthermore, it was shown that potassium cyanide, a potent inhibitor of polykaryon formation in the described system, inhibits an early step of membrane-membrane fusion of neighbouring cells.
has issue date	1987-01-01(xsd:dateTime)
bibo:doi	10.1007/bf01310786
bibo:pmid	3606395
has license	no-cc
sha1sum (hex)	b3439e6feaceb3440f387a79e514ae6fa9897f85
schema:url	https://doi.org/10.1007/bf01310786
resource representing a document's title	A novel method for the detection of early events in cell-cell fusion of Semliki Forest virus infected cells growing in monolayer cultures
has PubMed Central identifier	PMC7087209
has PubMed identifier	3606395
schema:publication	Arch Virol
resource representing a document's body	covid:b3439e6feaceb3440f387a79e514ae6fa9897f85#body_text
is schema:about of	named entity 'ARCHIVES' named entity 'fusion' named entity 'triggering' named entity 'growing' named entity 'CELL-CELL FUSION' named entity 'EARLY' named entity 'SEMLIKI FOREST VIRUS' named entity 'TO MEASURE' named entity 'SYSTEM' named entity 'HIGHLY' named entity 'DYE' named entity 'TO INVESTIGATE' named entity '30 MINUTES' named entity 'CONTRAST' named entity 'FUSION' named entity 'USED' named entity 'COMPLETED' named entity 'EARLY' named entity 'FUSING' named entity 'REPORT' named entity 'VIROLOGY' named entity 'CRITERION' named entity 'LOW PH' named entity 'FIXING' named entity 'DETECTION' named entity 'LUCIFER YELLOW' named entity 'INHIBITOR' named entity 'POTASSIUM CYANIDE' named entity 'INFECTED' named entity 'TRIGGERING' named entity 'USUALLY' named entity 'NOVEL' named entity 'AEDES ALBOPICTUS' named entity 'GLUTARALDEHYDE' named entity 'FLUORESCENT' named entity 'DIFFUSING' named entity 'MEMBRANE FUSION' named entity 'CELL-CELL FUSION' named entity '5 MINUTES' named entity 'FORMATION' named entity 'CELLS' named entity 'METHOD' named entity 'EVENTS' named entity 'OCCURRED' named entity 'NOVEL' named entity 'INFECTED' named entity 'MONOLAYER CULTURES' named entity 'SEMLIKI FOREST VIRUS' named entity 'RAPIDLY' named entity 'INDUCED' named entity 'MEMBRANE' named entity 'METHOD' named entity 'VIRUS' named entity 'DESCRIBED' named entity 'EVENT' named entity 'GROWING' named entity 'BRIEF' named entity 'STEP' named entity 'APPROXIMATELY' named entity 'CELLS' named entity 'fusion' named entity 'measure' named entity 'fusing' named entity 'cells' named entity 'Forest' named entity 'fluorescent' named entity 'Aedes albopictus' named entity 'Archives of Virology' named entity 'virus' named entity 'phase contrast microscopy' named entity 'Aedes albopictus' named entity 'potassium cyanide' named entity 'biochemical processes' named entity 'dividing cells' named entity 'PBS' named entity 'PBS' named entity 'potassium cyanide' named entity 'cell fusion'

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