About: High-resolution melting curve analysis: a novel method for identification of Mycoplasma species isolated from clinical cases of bovine and porcine respiratory disease

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About: High-resolution melting curve analysis: a novel method for identification of Mycoplasma species isolated from clinical cases of bovine and porcine respiratory disease Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	High-resolution melting curve analysis: a novel method for identification of Mycoplasma species isolated from clinical cases of bovine and porcine respiratory disease
Creator	Amanollahi, Reza Azari, Ania Farhid Hemmatzadeh, & Jozani, Razi Trott, Darren
source	PMC
abstract	Mycoplasma species cause wide ranges of infectious diseases in human and animals. The aim of the present study was to evaluate a real-time polymerase chain reaction (RT-PCR) followed by a high-resolution melting curve assay (HRM) for rapid differentiation of Mycoplasma species isolated from clinical cases of bovine and porcine respiratory disease. Lung samples from suspected cases to respiratory infections from cows and pigs were cultured on specific media, and the extracted DNA were tested by conventional polymerase chain reaction (PCR) assays for Mycoplasma. A set of universal primers specific for the 16S ribosomal RNA gene was designed and used for RT-PCR and HRM. The HRM analysis was able to differentiate between five different species of Mycoplasmas, namely, M. hyopneumoniae, M. bovis, M. hyorhinis, M. hyosynoviae and other uncultured Mycoplasma. All results were confirmed based on 16S rRNA gene sequencing. This rapid and reliable assay was as a simple alternative to PCR and sequencing, differentiating bovine and porcine mycoplasmas in species level.
has issue date	2019-11-01(xsd:dateTime)
bibo:doi	10.1007/s11250-019-02098-4
bibo:pmid	31673887
has license	no-cc
sha1sum (hex)	b3aec4b8c959ea67bd83332fca85ab68b1a3b945
schema:url	https://doi.org/10.1007/s11250-019-02098-4
resource representing a document's title	High-resolution melting curve analysis: a novel method for identification of Mycoplasma species isolated from clinical cases of bovine and porcine respiratory disease
has PubMed Central identifier	PMC7222993
has PubMed identifier	31673887
schema:publication	Trop Anim Health Prod
resource representing a document's body	covid:b3aec4b8c959ea67bd83332fca85ab68b1a3b945#body_text
is schema:about of	named entity 'HRM' named entity 'Mycoplasma' named entity 'suspected' named entity 'cultured' named entity 'CONVENTIONAL' named entity 'chain reaction' named entity 'rapid' named entity 'The' named entity 'HRM' named entity 'porcine' named entity 'M. bovis' named entity 'respiratory disease' named entity 'Mycoplasma' named entity 'assay' named entity 'cows' named entity 'Mycoplasma' named entity 'bovine' named entity 'porcine' named entity 'RT-PCR' named entity 'Mycoplasma' named entity 'RT-PCR' named entity 'M. hyorhinis' named entity 'bovine' named entity 'M. hyopneumoniae' named entity 'assay' named entity 'Mycoplasma' named entity 'melting curve analysis' named entity 'rapid' named entity '37°C' named entity 'spp' named entity 'respiratory disease' named entity 'DNA extraction' named entity 'bovine' named entity 'milliliter' named entity '16S ribosomal RNA' named entity 'veterinary sciences' named entity 'M. hyopneumoniae' named entity 'Qiagen' named entity 'denaturation' named entity 'Mycoplasma' named entity 'RTPCR' named entity 'Mycoplasma' named entity 'dairy industry' named entity 'Thermal Cycler' named entity 'gene' named entity 'porcine' named entity '37°C' named entity 'IFAT' named entity 'electrophoresis' named entity 'Lung' named entity 'M. hyorhinis' named entity 'Mycoplasma' named entity 'aseptically' named entity 'M. bovis' named entity 'speciation' named entity 'bovine' named entity 'porcine' named entity 'DNA extraction' named entity 'bovine' named entity 'agarose gel' named entity 'primer' named entity 'Germany' named entity 'PCR amplification' named entity 'positive and negative controls' named entity 'staining' named entity 'bovine milk' named entity 'PCR' named entity 'pvpA' named entity 'nmol' named entity 'Sanger' named entity 'serological tests' named entity '1:2' named entity 'bovine' named entity 'Mycoplasma' named entity 'PCR' named entity 'electrophoresis' named entity 'agar plate'

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