About: Nanofabrication of a gold fiducial array on specimen support for electron tomography

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Nanofabrication of a gold fiducial array on specimen support for electron tomography
Creator	Koster, Abraham Hagen, Cornelis Koning, Roman Kutchoukov, Vladimir
source	Elsevier; Medline; PMC
abstract	Abstract Here we describe the production, using lithography and micro-engineering technologies, of patterned arrays of nanofabricated gold dots on a thin Si3N4 electron transparent layer, supported by silicon. We illustrate that the support with a patterned structure of nanosized gold can be exploited for (cryo) electron tomography application as a specimen support with predefined alignment markers. This nanogold patterned support has several advantages. The Si3N4 window provides a 50nm thin, strong and flat support with a ∼0.7mm2 large electron-beam transparent window. The nanogold pattern has a user-defined size and density, is highly regular and stable. This facilitates accurate tracking during tilt series acquisition, provides sufficient contrast for accurate alignment during the image reconstruction step and avoids an uneven lateral distribution and movement of individual fiducials. We showed that the support is suitable for electron tomography on plastic sections.
has issue date	2013-12-31(xsd:dateTime)
bibo:doi	10.1016/j.ultramic.2013.06.015
bibo:pmid	23954496
has license	els-covid
sha1sum (hex)	b4382bfb15581e6c2313b6b69d25cfe87f307d0f
schema:url	https://doi.org/10.1016/j.ultramic.2013.06.015
resource representing a document's title	Nanofabrication of a gold fiducial array on specimen support for electron tomography
has PubMed Central identifier	PMC7111319
has PubMed identifier	23954496
schema:publication	Ultramicroscopy
resource representing a document's body	covid:b4382bfb15581e6c2313b6b69d25cfe87f307d0f#body_text
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