About: The intraspleen huPBL NOD/SCID model to study the human HIV-specific antibody response selected in the course of natural infection

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About: The intraspleen huPBL NOD/SCID model to study the human HIV-specific antibody response selected in the course of natural infection Goto Sponge NotDistinct Permalink

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	The intraspleen huPBL NOD/SCID model to study the human HIV-specific antibody response selected in the course of natural infection
Creator	Beirnaert, Els Donners, Helen Fransen, Katrien Heyndrickx, Leo Leroux-Roels, Geert Steyaert, Sophia Vanham, Guido Vanlandschoot, Peter Verhoye, Lieven
Source	Elsevier; Medline; PMC
abstract	Abstract The intrasplenic injection of human peripheral blood mononuclear cells (PBMCs) into severely immune deficient NOD/SCID mice, causes a massive and transient dominant expansion of human B cells in the spleen. This permits the easy isolation of human monoclonal antibodies specific for different antigens by a Kohler and Milstein-based method. Here we studied the human HIV-specific antibody response in the circulation of mice after intrasplenic transfer of PBMC from untreated HIV-infected patients with detectable to high viral load as well as from HAART-treated and from untreated patients, who kept an undetectable viral load (the latter referred to as “natural suppressors”). Excellent B cell expansion was obtained for all PBMC. High level replication of virus was observed after transfer of PBMC of untreated viremic patients only. A strong and multispecific HIV-specific antibody response was observed after transfer of PBMC of untreated viremic patients and natural suppressors. In contrast, only a weak and pauci-specific antibody response was detected in mice reconstituted with PBMC from successfully treated patients. Based on these observations we conclude that the use of the intraspleen mouse model confirmed a) the presence of HIV-specific circulating memory B cells in untreated patients and natural suppressors; b) the nearly complete absence of circulating memory B cells in patients receiving highly active antiretroviral therapy. Using the intraspleen model we generated large numbers of immortalized B cells and isolated two anti-p24 human monoclonal antibodies. We further conclude that the intraspleen huPBL NOD/SCID model is a small animal model useful for the analysis of the antibody response against HIV found in patients.
has issue date	2007-03-30(xsd:dateTime)
bibo:doi	10.1016/j.jim.2006.12.003
bibo:pmid	17258763
has license	els-covid
sha1sum (hex)	b49725e4ca8f930a39cec5c3088976f1a132edfa
schema:url	https://doi.org/10.1016/j.jim.2006.12.003
resource representing a document's title	The intraspleen huPBL NOD/SCID model to study the human HIV-specific antibody response selected in the course of natural infection
has PubMed Central identifier	PMC7125539
has PubMed identifier	17258763
schema:publication	Journal of Immunological Methods
resource representing a document's body	covid:b49725e4ca8f930a39cec5c3088976f1a132edfa#body_text
is schema:about of	named entity 'patients' named entity 'generated' named entity 'B cells' named entity 'B cell' named entity 'complete' named entity 'REFERRED TO' named entity 'NEARLY' named entity 'OBSERVATIONS' named entity 'EXPANSION' named entity 'MOUSE MODEL' named entity 'SPECIFIC' named entity 'KOHLER' named entity 'IMMORTALIZED' named entity 'CIRCULATING' named entity 'MASSIVE' named entity 'OBTAINED' named entity 'REPLICATION' named entity 'METHOD' named entity 'CIRCULATION' named entity 'EXCELLENT' named entity 'FOUND' named entity 'PATIENTS' named entity 'MICE' named entity 'USEFUL' named entity 'ANTIBODY RESPONSE' named entity 'HIGH' named entity 'IMMUNE' named entity 'VIRUS' named entity 'SEVERELY' named entity 'RECONSTITUTED' named entity 'SPECIFIC ANTIBODY' named entity 'CONTRAST' named entity 'MODEL' named entity 'PERMITS' named entity 'CONCLUDE' named entity 'LARGE' named entity 'P24' named entity 'GENERATED' named entity 'NOD' named entity 'MODEL' named entity 'B CELL' named entity 'SMALL ANIMAL' named entity 'PRESENCE OF' named entity 'INJECTION' named entity 'WEAK' named entity 'HIGHLY ACTIVE ANTIRETROVIRAL THERAPY' named entity 'HAART' named entity 'USING' named entity 'NUMBERS' named entity 'NOD' named entity 'ANIMAL MODEL' named entity 'USE OF' named entity 'DETECTED' named entity 'IS A' named entity 'ANTIBODIES SPECIFIC' named entity 'ISOLATION' named entity 'TRANSIENT' named entity 'RECEIVING' named entity 'DETECTABLE' named entity 'ANALYSIS' named entity 'STUDY' named entity 'HUMAN' named entity 'BASED' named entity 'TREATED' named entity 'THESE' named entity 'ISOLATED' named entity 'NATURAL INFECTION' named entity 'COURSE' named entity 'ANTIBODY RESPONSE' named entity 'HIV' named entity 'SPECIFIC ANTIBODY' named entity 'VIRAL LOAD' named entity 'OBSERVED'

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