About: Cell‐Based Hepatitis C Virus Infection Fluorescence Resonance Energy Transfer (FRET) Assay for Antiviral Compound Screening

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Cell‐Based Hepatitis C Virus Infection Fluorescence Resonance Energy Transfer (FRET) Assay for Antiviral Compound Screening Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Cell‐Based Hepatitis C Virus Infection Fluorescence Resonance Energy Transfer (FRET) Assay for Antiviral Compound Screening
Creator	Yu, Xuemei Uprichard, Susan
source	Medline; PMC
abstract	Hepatitis C virus (HCV) affects an estimated 3% of the population and is a leading cause of chronic liver disease worldwide. Since HCV therapeutic and preventative options are limited, the development of new HCV antivirals has become a global health care concern. This has spurred the development of cell‐based infectious HCV high‐throughput screening assays to test the ability of compounds to inhibit HCV infection. This unit describes methods that may be used to assess the in vitro efficacy of HCV antivirals using a cell‐based high‐throughput fluorescence resonance energy transfer (FRET) HCV infection screening assay, which allows for the identification of inhibitors that target HCV at any step in the viral life cycle. Basic protocols are provided for compound screening during HCV infection and analysis of compound efficacy using an HCV FRET assay. Support protocols are provided for propagation of infectious HCV and measurement of viral infectivity. Curr. Protoc. Microbiol. 18:17.5.1‐17.5.27. © 2010 by John Wiley & Sons, Inc.
has issue date	2010-08-01(xsd:dateTime)
bibo:doi	10.1002/9780471729259.mc1705s18
bibo:pmid	20812217
has license	no-cc
sha1sum (hex)	b5f04c924b010856ff8d0d058a74fd6b34c89106
schema:url	https://doi.org/10.1002/9780471729259.mc1705s18
resource representing a document's title	Cell‐Based Hepatitis C Virus Infection Fluorescence Resonance Energy Transfer (FRET) Assay for Antiviral Compound Screening
has PubMed Central identifier	PMC2964379
has PubMed identifier	20812217
schema:publication	Curr Protoc Microbiol
resource representing a document's body	covid:b5f04c924b010856ff8d0d058a74fd6b34c89106#body_text
is schema:about of	named entity 'inhibitors' named entity 'protocols' named entity 'care' named entity 'preventative' named entity 'assays' named entity 'MAY BE' named entity 'UNIT' named entity 'COMPOUND' named entity 'ASSAY' named entity 'FRET' named entity 'HEALTH CARE' named entity 'LIMITED' named entity 'ANALYSIS' named entity 'HCV INFECTION' named entity 'USED' named entity 'TO TEST' named entity 'propagation' named entity 'HCV' named entity 'antivirals' named entity 'high-throughput' named entity 'HCV' named entity 'fluorescence' named entity 'HCV' named entity 'resonance' named entity 'Since' named entity 'assay' named entity 'ability' named entity 'step' named entity 'HCV' named entity 'HCV' named entity 'antivirals' named entity 'fluorescence resonance energy transfer' named entity 'HCV' named entity 'infection' named entity 'HCV' named entity 'HCV' named entity 'HCV' named entity 'high-throughput' named entity 'assay' named entity 'HCV' named entity 'Infection' named entity 'Fluorescence Resonance Energy Transfer' named entity 'Hepatitis C Virus' named entity 'pathogen' named entity 'posttranslationally' named entity 'infection' named entity 'clonal' named entity 'RNase inhibitor' named entity 'EDANS' named entity 'reproducibility' named entity 'ribavirin' named entity 'peptide' named entity 'FRET' named entity 'RNA' named entity 'pipettor' named entity 'HCV' named entity 'QXL' named entity 'positive and negative controls' named entity 'spectrophotometry' named entity 'HCV' named entity 'DMSO' named entity 'Spin' named entity 'Hepatitis' named entity '5-FAM' named entity 'DMSO' named entity 'agarose gel electrophoresis' named entity 'titer' named entity 'cell lines' named entity 'Inoculate' named entity 'infection' named entity 'methylcellulose' named entity 'cytopathic effect' named entity 'transfection' named entity 'mycoplasma' named entity 'HTS' named entity 'polyprotein' named entity 'endogenous' named entity 'electroporation' named entity 'IFN' named entity 'tissue culture'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2025 OpenLink Software