About: Antibody epitope repertoire analysis enables rapid antigen discovery and multiplex serology

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Antibody epitope repertoire analysis enables rapid antigen discovery and multiplex serology Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Antibody epitope repertoire analysis enables rapid antigen discovery and multiplex serology
Creator	Daugherty, Patrick Gable, Cameron Handali, Sukwan Johnston, Timothy Kamath, Kathy Mcauliffe, Isabel Pantazes, Robert Reifert, Jack Rivera, Hilda
Source	PMC
abstract	The detection of pathogen-specific antibodies remains a cornerstone of clinical diagnostics. Yet, many test exhibit undesirable performance or are completely lacking. Given this, we developed serum epitope repertoire analysis (SERA), a method to rapidly discover conserved, pathogen-specific antigens and their epitopes, and applied it to develop an assay for Chagas disease caused by the protozoan parasite Trypanosoma cruzi. Antibody binding peptide motifs were identified from 28 Chagas repertoires using a bacterial display random 12-mer peptide library and next-generation sequencing (NGS). Thirty-three motifs were selected and mapped to candidate Chagas antigens. In a blinded validation set (n = 72), 30/30 Chagas were positive, 30/30 non-Chagas were negative, and 1/12 Leishmania sp. was positive. After unblinding, a Leishmania cross-reactive epitope was identified and removed from the panel. The Chagas assay exhibited 100% sensitivity (30/30) and specificity (90/90) in a second blinded validation set including individuals with other parasitic infections. Amongst additional epitope repertoires with unknown Chagas serostatus, assay specificity was 99.8% (998/1000). Thus, the Chagas assay achieved a combined sensitivity and specificity equivalent or superior to diagnostic algorithms that rely on three separate tests to achieve high specificity. NGS-based serology via SERA provides an effective approach to discover antigenic epitopes and develop high performance multiplex serological assays.
has issue date	2020-03-24(xsd:dateTime)
bibo:doi	10.1038/s41598-020-62256-9
bibo:pmid	32210339
has license	cc-by
sha1sum (hex)	b673c971353653abcb589c3b57dd0703eb3c9cc9
schema:url	https://doi.org/10.1038/s41598-020-62256-9
resource representing a document's title	Antibody epitope repertoire analysis enables rapid antigen discovery and multiplex serology
has PubMed Central identifier	PMC7093460
has PubMed identifier	32210339
schema:publication	Sci Rep
resource representing a document's body	covid:b673c971353653abcb589c3b57dd0703eb3c9cc9#body_text
is schema:about of	named entity 'DETECTION' named entity 'TRYPANOSOMA CRUZI' named entity 'EPITOPES' named entity 'INCLUDING' named entity 'PEPTIDE LIBRARY' named entity 'THEIR' named entity 'ASSAY' named entity 'NEGATIVE' named entity 'NON-' named entity 'PATHOGEN' named entity 'PERFORMANCE' named entity 'IDENTIFIED' named entity 'LEISHMANIA' named entity 'SECOND' named entity 'CHAGAS' named entity 'MOTIFS' named entity 'CROSS-REACTIVE' covid:arg/b673c971353653abcb589c3b57dd0703eb3c9cc9 named entity 'antigen' named entity 'antigens' named entity 'Antibody' named entity 'immunoprecipitation' named entity 'epitopes' named entity 'motif discovery' named entity 'antibody' named entity 'antigen' named entity 'multiplexed' named entity 'Bolivia' named entity 'serology' named entity 'testing laboratories' named entity 'data sets' named entity 'algorithm' named entity 'v1.1' named entity 'standard deviation' named entity 'v1.0' named entity 'epitopes' named entity 'antigens' named entity 'epitope' named entity 'quality control' named entity 'epitopes' named entity 'pathogen' named entity 'nature.com' named entity 'Epitope' named entity 'phage display library' named entity 'peptides' named entity 'peptide' named entity 'v1.0' named entity 'epitope' named entity 'z-score' named entity 'heart failure' named entity 'z-score' named entity 'Chagas disease' named entity 'motif' named entity 'bacterial culture' named entity 'epitope' named entity 'epitope' named entity 'antibody repertoire' named entity 'CLIA' named entity 'antigens' named entity 'peptide' named entity 'CDC' named entity 'Chagas' named entity 'Chagas' named entity 'epitopes' named entity 'asymptomatic' named entity 'epitopes' named entity 'Sensitivity and specificity' named entity 'NGS' named entity 'substitution matrix' named entity 'antibody' named entity 'peptide library' named entity 'in-frame' named entity 'serology'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software